Parts
To aid future iGEM teams we have listed genes, plasmids and constructs used in our project as BioBricks parts. In all, we have contributed 16 parts - of which 12 are basic and 4 are composite parts. We believe that the parts submitted by our team will help young researchers and professionals in designing experiments.
Basic Parts
The parts here can be classified as follows:
Coding type
a) Part no. BBa_K4382000 and BBa_K4382001 are cellulase genes.
b) Part no. BBa_K4382002 is a ligninase gene.
c) Part no BBa_K4382006 to BBa_K4382008 are pectinase genes.
d) Part no BBa_K4382003 to BBa_K4382005 and BBa_K4382010 are xylanase enzymes.
RBS type
Part no BBa_K4382009 is a Bacillus subtilis Ribosome binding protein
Plasmid type
- Part no BBa_K4382015 is p Partial Sequence of pCri-18a plasmid with additional SalI site
Composite parts
The composite parts consist of the basic parts assemble together based on their functionality. There are four composite parts - xylanase, cellulase, ligninase and pectinase; which are numbered from BBa_K4382011 to BBa_K4382014.
Soil Health Handbook
There are several communities who have not got exposure to the grass root level agricultural problems. As, we belong to an agricultural country we decided these agricultural problems should be addressed at each and every level. Moreover, the highschool iGEM teams can also infer to this handbook as this takes the agricultural issue from a basic level to the higher. Our handbook is mainly focused on soil health. That will consider the deteriorating soil health due to stubble burning and antibiotic addition through manure application.
Our Handbook puts light on:
- Importance of farmers
- How population growth is one of the main cause of increasing demand of food.
- For higher and frequent production of crops how farmers overuse the available chemical products in the market, which eventually lead to the soil health degradation.
- The past data of degrading soil health consequences due to stubble burning and antibiotic overuse.
- Hence, Our solution.
This handbook is designed, as to make people linked with agricultural practices like farmers, industries aware of these issues. The crisp content of the handbook would also appeal to school students to instill their interest in agriculture.
Bacillus subtilis handbook
Our team has made a handbook on handling of Bacillus subtilis strain in laboratory. We have comprehensively provided the list of all protocols for culturing and transformation of Bacillus subtilis strain which we used in our experiments. These protocols helped up obtain transformed colonies very efficiently and in lesser time, so we believe this handbook will be very useful for anyone working with Bacillus subtilis.