Collaboration with HainanU_China
Click the link below to learn more about
HainanU_China.
In the middle of the project, we encountered HainanU_China. Based on the common ground of utilizing
CRISPR system, we further communicated in terms of human practices, wiki building, and artwork
layout,
etc. We continued to improve our respective projects during these exchanges, and reached good
partnership. It is definitely a wonderful and happy experience with HainanU_China.
Figure 1. The logos of HainanU_China and UESTC_BioTech.
Midterm of our project: meeting HainanU_China
During an online meeting, we learned that HainanU_China plans to use the CRISPR-13 system to construct
of
the nucleic acid detection platform, which is very significant in the global pandemic context and
relevant
to the technology used in our project. Based on
the aspect of CRISPR technology, we reached a
preliminary
intention to deepen our communication.
Figure 2. The first meeting online to do the respective introduction of our projects.
Early August: Preparation for CRISPR meetup
We learned that many Chinese iGEM teams use the CRISPR system. In order to benefit wider community
as
well
as discussing CRISPR related technology more comprehensively , we invited CPU_China, SCU-China, BIT
DUT_China, HiZJU-China, NWU-CHINA-A, SCAU_China, and SJTU-software and other teams to share their
projects. Besides, We also invited three speMid-termsts to give comment on our presentation and
discussion. They are Yang Jianzhe who acted as iGEM Ambassador in 2021, Liu Jingrong who has been
tutoring
more than 20 iGEM teams and ShiSonglin who is a member of Biodiversity Leadership Program and familiar
with CRISPR system. Apart from inviting teams, we often met online to settle down details of
meetup.
To be
specific, we distributed tasks consisting of making invitation letters, designing application form,
writing hosts word, noting down minutes and so on.
Figure 3. Online meeting for preparing our meetup.
August 14th: CRISPR meetup in progress
After doing full preparation beforehand, we hosted this meetup together on August 14. With 8 other
iGEM
teams and three special guests, we did extensive discussions on experiment, modeling, wiki, and human
practice respectively and sought advice and solutions for one another, during which process we built
closer friendships. These questions and answers, as well as the title and abstract of each team, were
finally compiled into a brochure for future reviewing and experience accumulation of iGEM competition.
The
official account of CCiC community reposted our meetup summary to propagandize and recognize our
efforts.
Figure 4. CRISPR conference brochure and screenshots of the meeting.
Later stage of the project: giving advice and continuous improvement
In the later stage of the project, we both encountered a lot of difficulties in terms of the
presentation
of the project to the public,the construction of the wiki, the layout of the artwork and the execution
of
Human Practice.The relevant persons in charge on both sides shared ideas and solutions, so as to
achieve
better results on respective projects. For instance, when HainanU_China got stuck in writing iGEM
brochure
for experience sharing, we offered them precious sample from our former iGEMer and joined them in the
compilation of
brochure for
sharing iGEM related experience.
Figure 5. Cooperation in making the hand book.
Collaboration with Sorbonne_U_Paris
Figure 6. The adjusted team logo to match the color tone of Sorbonne_U_Paris.
During the early process of extensive communication, we are lucky enough to find our
intimate
partner
Sorbonne_U_Paris.
First meeting: reaching consensus on establishing partnership.
We surprisingly found that we use the same chassis organism--Chlamydomonas reinhardtii. After meeting
for
the first time on Zoom to introduce our projects respectively, we learn about the project of each
other
and reached a consensus on establishing an in-depth partnership. With regards to the project of
Sorbonne_U_Paris, their project this year is to use Chlamydomonas reinhardtii as a food source for
treating iron-deficiency anemia.
Figure 7. The first meeting to introduce our projects respectively.
Midterm of our project: Sorbonne_U_Paris tutored us to design a killswitch
Through extensive discussion on CCiC, our team decided to design a killswitch to enhance our safety
level
by designing a killswitch. But we lack experience in this aspect. After hearing our dilemma,
Sorbonne_U_Paris warm-heartedly tutored the whole design of the switch.
Figure 8. Previous files of killswitch designing provided by Sorbonne_U_Paris.
Midterm of their project: We provided crucial genes for them.
While they were doing their project, they found it tricky to remove chlorophyll to make their algae
tastier, under which circumstance we suggested the knockout of the PSY1 gene which is closely related
to
chlorophyll synthesis and provided them with its references, specific information, constructed plasmid
and
designed locus of sgRNA.
Figure 9. The various data of PSY1 gene we provided for Sorbonne_U_Paris.
In the beginning of September: modeling construction help
In the relative later period of our project, Sorbonne_U_Paris dedicated itself to constructing
modelling
for simulating the growth curve of C.reinhartii. And they needed data of the growth curve of
C.reinhartii
to complete their calibration. After learning that, we provided them with it to enrich their data for
modeling construction.
Figure 10. Providing growth curve for Sorbonne_U_Paris.
In the late September, GMOs video making
To further deepen our collaboration and communication, we collectively did a research on the
GMOs(Genetically Modified Organisms) regulations in respective country, and finally summarized the
essence
we learned in the form of an video. It is of vital importance because we can make our potential users
of
GMOs project learn about the policy context in different countries, which contributes to further
education
and communication in distinctive regions in the realm of GMOs.
