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Traditionally, biofuels are made from oil extracted from plants represented by beans, corn, and oilseed rapes, which would take up farmland to grow therefore conflicting with human's basic needs for food .
Another highly commercialized way of producing biofuels is to recycle animal oil, which is cheap but rather than pure .

Recent years have witnessed the advancement of another source for biofuel production, namely Microbes . Microbes represented by microalgae, yeast and mold are excellent resources for producing lipid .
Using microbes to produce biofuels can demonstrate significant advantages, namely high productivity, little room requirement, and no natural environment limitation.