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Overview


Non-achievement can be realized without collaboration and help from each other. This year, our team is made up of 4 PIs, 1 instructor, 2 student leaders, 13 student members, and 3 advisors, each of whom has distinguished contributions to our project. Meanwhile, we received help from fellow researchers, fellow iGEM teams, business companies, government bureaus and departments, etc. We couldn't have done so much without close cooperation and generous assistance from everyone mentioned above.

PIs


Our PIs gave instructions and directed the design and construction of our project.
Zhang Yong
Professor Zhang is the primary PI of our team. Dedicated to research around plant genome engineering for decades, he provided us with information and thoughts about how to design the project, when to do what, and whom to look for help. He is such an important character in the whole process.
Tang Lixia
Professor Tang gave us suggestions around metabolism, enzyme research and modelling. She often offered us guidance on how to analyze massive metabolic pathways and how to decide which gene to manipulate. Also, she led us through wiki modifications and improvements.
Zheng Xuelian
Professor Zheng offered great assistance when it comes to the culture of chassis organism and experiment technique. She always made sure we are on the right path of experiments and provided advice on wiki and presentations. She also helped us a lot in human practices such as card copyright application and etc.
Feng Juan
Professor Feng taught us how to use many laboratory equipments and offered much assistance on chemical analysis methods for example how to get brighter fluorescent images and how to analyze lipid contents. She also made sure of members' mental health and provided constant support.

Instructor


Quan Ling
Quan helped the team leaders to coordinate the whole team and secured financial support for the project. She also provided tons of assistance in human practices including designing the process, introducing us to government departments, building connections with science propagation platforms and manpower support.

Advisors


Tang Xu
Tang designed the standard CRISPR plasmid for us and taught us how to construct and examine plasmids. Also, he provided us many tips on molecular biology experiments.
Hu Yuwei
Hu is one of the student leaders of UESTC_China in the 2021 iGEM competition. Without her, our team couldn't become as united as we are now and we won't be able to get in track so soon. She always made sure we understood the regulations and instructions of the whole competition and she gave much advice on how to perfect our wiki and presentation.
Zhou Ying
Zhou is also a student leader of UESTC_China in 2021. Her capability in wet lab designs and organization blew our minds. From an early stage, she taught us how to arrange lab work so that we could complete a considerable amount of work. Whenever we have questions about lab management, we always come to her for help.

Acknowledgements


School

Special thanks to the University of Electronic Science and Technology (UESTC), UESTC Academic Affairs Office, School of Life Science and Technology, and Education Development Foundation of UESTC for all the support.

Sponsors

Special thanks to 23mofang and Snapgene for their kind sponsorship. Without their donations and software support, we would not have achieved so much.

Cooperation in science education

Special thanks to UESTC National Science Park, Sichuan Science and Technology Museum, Chengdu Ecological Environment Bureau especially the Communication and Education Centre, and Chengdu Environmental Volunteer Services Association for their assistance and support in our science education, communication and propagation work. Also, thanks to Tongxinzhiyuan social practice team of UESTC for the support in education events.

Cooperation in survey and research

Special thanks to the following scholars for their generous response to our survey on research status, industry prospects and development directions.
  • Professor Zhang Zhaohan from the Department of Ecology and Environment, Harbin Institute of Technology;
  • Advanced Engineer Ma Nian, from T. Y. Lin International;
  • Shi Dongshen, from Xuzhou Yuanheng Renewable Energy Development Corporation;
  • Pro. Liu Tianzhong from the key laboratory of bio-fuels in the Chinese Academy of Sciences.
  • And Qing Dao FuRuiSi Biodiesel Enterprise.
    Thank you!

    Cooperation in Biology Lab work

    Special thanks to Jigu Gene for completing 9 gRNAs inserted CRISPR plasmids for us, and Vazyme Biotech for kindly offering us plant DNA extraction kits to test its function on algae.

    Team members


    All the members are divided into 5 groups: Managing group, Bio department, Human practices group, Wiki and design group, and Modelling group.

    Managing group

    Luo Yuning: Luo is the primary student leader. She is responsible in coordinating the whole progress of the team and checking iGEM official website's updates to make sure the team follow every regulation and hand in all the materials on time. She is also in charge of financial management.
    Wang Xingjie: Wang is the co-leader of the team and the manager of the bio department. He is mainly in charge of lab management, experiments arrangement, and communication with PIs and advisors.

    Bio department

    Wang Xingjie: As the manager of the department, Wang assigns experiments to specific members, arranges meetings, manages the whole lab including replenishing laboratory consumables and reagents and repairing laboratory equipment, and builds connections with other labs in the School of Life Science and Technology of UESTC. He also designs and constructs CRISPR/Cas9 plasmids with Zhang Hang. And he organizes the whole department to record lab work and finish the notebook.
    Feng Ying: Feng is specialized in the electronic transformation and pressure cultivation of algae. Her work greatly supports our genome engineering process. She also concludes the protocol of lipid extraction and KEGG user's guide.
    Hu Boyan: Hu also focuses on the electronic transformation of algae. She concludes possible target genes on lipid droplet degradation pathway and TAG synthesis pathway.
    Yang Qianwen: Yang mainly finishes plasmids extraction and preparation for transformation and transcriptome analysis data processing. She also works with the modelling group to construct a specific formula that describes the relationship between OD750 and algae biomass.
    Sun Shengyu: Sun mainly finishes plasmids extraction and preparation for transformation and transcriptome analysis data processing.
    Feng Zhenxing: Feng is in charge of Nile red dying, pressure conditions and glass beads transformation of algae. He makes sure we can get clean and clear fluorescence images.
    Zhang Hang: Zhang is the main member of plasmids construction and measurement. He also established the whole protocol on the cultivation and generation of the Chlamydomonas reinhardtii. He also helps with other experiments that needed to be done.
    Luo Yuning: Luo takes part in algae cultivation both in normal and pressure conditions.

    Human practices group

    Wang Shenglan: Wang is the leader of the whole group. She is the main character in integrated human practices, inclusivity, education and communication, collaboration and partnership. She interviews several government departments, companies and scholars to find the true meaning of our project. She designs and leads the development of Algal classes. She seeks collaboration with all the stakeholders we are possible to reach out to.
    Feng Zhenxing: Feng designs the card game Algal Lab which strongly promotes our education works. Feng also helps with Algae classes' live shows.
    Luo Yuning: Luo runs social media such as Weibo, Instagram, Wechat, and Twitter for the team. She also helps with collaboration and partnership and proactively communicates with different iGEM teams.

    Wiki and design group

    Sun Yusong: Sun designs and makes our wiki websites.
    Ai Xiyue: Ai designs and makes our wiki websites.
    Zeng Kai: Zeng designs and produces wiki websites, makes the card game, designs team shirts, and edits all the videos.
    Jin Haoran: Jin takes photos for wiki websites and the bio department, draws the team logo, and films all the videos.

    Modelling group

    Xiao Rongxing: Xiao constructs the Genome-scale Metabolic model and CRISPR off-target model. He also seeks modelling cooperation with Sorbonne_U_Paris and DUT_China. Xiao also comes up with a formula that describes the relationship between OD750 and algae biomass.
    Qiao Jiawei: Qiao constructs the Genome-scale Metabolic model and CRISPR off-target model. He also seeks modelling cooperation with Sorbonne_U_Paris and DUT_China.