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Overview

We registered a total of 19 parts this year, including 12 basic parts,5 composite parts and 2 plasmids, and most of them have been tested to ensure their reliability.

Basic Parts

This year we have made 12 basic parts, most of which are the basic components of our synthetic vector pTX2038 and plasmid pTX2040, including various codings, functional sequences, promoters and terminators.

Short Discription Part Name Part Type Function Length
ChlamyHgR BBa_K4335003 Coding Hyg resistant, used as a screening gene for plasmids 1144
StayGold BBa_K4335002 Reporter A new GFP, which was discovered in 2022 as a reporter 654
mCherry BBa_K4335004 Reporter A red fluorescent protein used as a reporter 865
RBCS2 promotor BBa_K4335000 Promotor Expression of Promoter Resistance Gene and Fluorescent Protein 201
RBCS2 term BBa_K4335006 Terminator Terminate gene expression 234
3xFlag+NLS BBa_K4335014 Coding Labeling and nuclear localization sequence of Cas9 protein 120
HSP70A Promotor BBa_K4335008 Promotor Drive the expression of Cas9 protein 91
insert site+gRNA scaffold BBa_K4335009 Coding The site where sgRNA is inserted, expressing sgRNA and binding it to Cas9 107
KanR BBa_K4335011 Coding Expression of kanamycin resistance as a marker gene 250
pCrU6.3 BBa_K4335012 Promotor Drive the expression of gRNA 646
SpCas9 BBa_K4335013 Coding The expression of Cas9 protein 4101
polyT Term BBa_K4335015 Terminator Terminate gene transcription 7

Composite Parts

This year, we have made six composite parts. Most of the six basic parts are independent functional units of our synthetic plasmid pTX2038 and plasmid pTX2040, Cas9 system and fluorescence system.

Short Discription Part Name Function Part Type Diagram Length
HSP70A
Promoter+3xFlag
+NLS+SpCas9+
NLS+Rbcs2
Term 		BBa_K4335020 Expression of
Cas9 protein with
NLS and Flag 		Composite 4606
pCrU6.3+insert
site+SpScaffold
+polyT Term 		BBa_K4335021 Transcription
of sgRNA 		Composite 759
Rbcs2Promotor+
ChlamyHgR
+Rbcs2 Term 		BBa_K4335022 Expression of
hygromycin
resistance gene 		Composite 1797
Rbcs2 Promotor
+mCherry+Rbcs2
Term 		BBa_K4335023 Expression of
mCherry red
fluorescent
protein 		Composite 1331
Rbcs2 Promotor+
Staygold+Rbcs2
Term 		BBa_K4335024 Expression of
StayGold green
fluorescent
protein 		Composite 1274