This year, we managed to meet every medal criterion and put effort into many awards. This page is made to ease judges while reviewing.

We complete the following Competition Deliverables: Wiki, Project Promotion Video, Team Presentation, and Judging Form.

We clearly describe our team members' contributions and other people's devotion to the project. Read more at https://2022.igem.wiki/uestc-biotech/attributions.

The accelerated rising of Earth's average air temperature due to the combustion of fossil fuels has had disastrous effects on the global climate. It's vital for the world to respond to the issue together. We focus on using microalgae to produce biofuel in a carbon-neutral way. Explore more at https://2022.igem.wiki/uestc-biotech/description.

This year, we selected Chlamydomonas reinhardtti as the chassis organism. We knock down the genes related to lipid production, namely LACS1, LACS2, PLA2, ACX2, PEPC1, DTH1, and CHT7 genes in its metabolic pathway by utilizing the CRISPR-CAS9 system, so as to achieve the purpose of efficient lipid production. Read more at https://2022.igem.wiki/uestc-biotech/contribution.

We engineered a biological system in Chlamydomonas reinharditii based on CRISPR/Cas9 system. Explore https://2022.igem.wiki/uestc-biotech/engineering to know how we complete the DBTL cycle.

We value the connection within the large iGEM community, during which process we can troubleshoot our tricky problems as well as gain invaluable friendships. Detailed collaborations at https://2022.igem.wiki/uestc-biotech/collaborations.

By “walking” in the world by Human Practice, our micro-algae could respond to the real world's need and pose greater impact on the world. Read more at https://2022.igem.wiki/uestc-biotech/human-practices.

To get a better idea of how Chlipid should and would be implemented in the real world, we researched the current state of the industry, identified application prospects and anchored application targets through multiple routes. Combining what we have learned from the real world and what we have achieved in the lab, we propose implementation methods at https://2022.igem.wiki/uestc-biotech/implementation.

We develop Human Practices into Integrated Human Practices. Read more at https://2022.igem.wiki/uestc-biotech/human-practices.

We improved 2 existing parts BBa_K2984012 and BBa_K2136016 into BBa_K4335003 and BBa_K4335004. Read more at https://2022.igem.wiki/uestc-biotech/improve.

To gain a deeper understanding of the physical mechanisms behind gene editing, we develop a thermodynamics-based model to predict the potential off-target activity of the selected guide RNA. And the model we use overcomes the above binary limitations of the previous physics-based binary off-target predicting model and considers off-targets effects with both high possibility and low possibility. Read more at https://2022.igem.wiki/uestc-biotech/model.

Based on the implementation, we present proof of concept with detailed experiment results that mainly prove Chlipid's foundational research system can work in a relevant context. Read more at https://2022.igem.wiki/uestc-biotech/proof-of-concept.

We establish close partnerships with HainanU_China and Sorbonne_U_Paris. Read more at https://2022.igem.wiki/uestc-biotech/partnership.

In order to take responsibility for various stakeholders, we did a wide range of communication to consult the advice and feedback for our project, contributing to the gradual amelioration of our project. Significant also, to invite more people to the synthetic biology world and let wider scope of the audience know about the magic power of micro-algae, we did scientific popularization by designing a series of algae and synthetic biology-related classes. Read more on https://2022.igem.wiki/uestc-biotech/communication.

To make the world know and acknowledge the unique value of our project, our center of education is about microalgae, genetic engineering technology and the unique significance of our project. Three striking characteristics could be spotted in our education efforts, namely three INs (informative, innovative, and inclusive). Let us view our efforts in detail at https://2022.igem.wiki/uestc-biotech/education.

In Chlipid, we emphasized the importance of inclusivity. We focus on building a diverse synthetic biology community where everyone can be involved, learn and express themselves. Read more on https://2022.igem.wiki/uestc-biotech/inclusivity.

The whole process of our integrated human practice was segmented into five parts, namely practical significance, technical consultancy, bio-safety issues, policy and law, and industry research, during which procedures the human practice acts as indispensable guidance for finding the unique value, centering our project, enhancing biosafety, securing the lawful environment and learning realistic problems of our project. Read more at https://2022.igem.wiki/uestc-biotech/human-practices.

As we all know, the mathematical models are an effective tool for exploring experimental conditions and verifying experimental results. In our project, Chlamydomonas reinhardtii is raised by gene editing to achieve optimal TAG accumulation. We developed a thermodynamics-based model to predict the potential off-target activity of the selected guide RNA. And the model we use overcomes the above binary limitations of the previous physics-based binary off-target predicting model and considers off-targets effects with both high possibility and low possibility. Read more at https://2022.igem.wiki/uestc-biotech/model.

We present BBa_K4335002, a newly discovered fluorescent protein as a compatible part for the awards. Read more at http://parts.igem.org/Part:BBa_K4335002.

We present BBa_K4335024, Rbcs2 Promotor+Staygold+Rbcs2 Terminator, as a compatible part for the awards. Read more at http://parts.igem.org/Part:BBa_K4335024.

We innovatively construct a CRISPR/Cas9 system that is especially for Chlamydomonas reinhardtii in great detail. Not only have we successfully proved the feasibility of the genome editing tool in algae, but we also bring out omics research that assists researchers to discover target genes and construct a CRISPR off-target model to help search for a suitable gRNA. Read more at https://2022.igem.wiki/uestc-biotech/plant.

In Chlipid, we emphasize the importance of sustainable development impact. We design Chlipid aiming for 8 SDGs, and by close cooperating with other iGEM teams, we contribute to 11 SDGs in total, namely Zero Hunger (SDG2), Good Health and Well-being (SDG3), Quality Education (SDG4), Clean Water and Sanitation (SDG6), Affordable and Clean Energy (SDG7), Decent Work and Economic Growth (SDG8), Industry, Innovation and Infrastructure (SDG9), Sustainable Cities and Communities (SDG11), Responsible Consumption and Production (SDG12), Climate Action (SDG13), and Partnerships for the Goals (SDG17). Read more at https://2022.igem.wiki/uestc-biotech/sustainable.

In order to prevent leakage of engineered organisms, we strictly perform sterilization before disposing of any engineered organisms in the lab. We also collaborated with team Sorbonne_U_Paris to design a kill switch to secure the process. Besides, we pay attention to safe experiment design and laboratory practices to ensure the safety of our project and the safety of our members. Read more at https://2022.igem.wiki/uestc-biotech/safety.