The High Throughput Electro Actuator (HTEA) is a custom made, specifically conceptualised, designed and produced by our team in the context of iGEM, inexpensive Printed Circuit Board (PCB) harbouring an array of 96 pairs of interchangeable electrodes matching the layout of a standard 96 well plate. Working in conjunction with the AC Dispatcher (ACD) and the Electro Planner, this device allow to dispatch AC and DC current to each individual well, either one at the time or row by row.

The 5, individually gated signals received from the ADC are dispactched to 12 CD4051B multiplexors (MUX). Each MUX is ten distributintng the incoming signal to either of the 8 wells in a column or to a non connected well (completly gating out the signals). This multi level of gating (at the relay level on the ADC and on each MUX) assure no leackage to individual electrodes.

Addressing of the MUX is done through 3 I2C MCP23017 IO Expander

We used the HTEA in conjuntion with the (ACD) and the Electro Planner in the Screening of electrically active promoters experiment, The Impact on Growth of electroshock experiement, Effect of electroshock on Vmem experiment.