Protocols
- Bacillus Subtilis Transformation {DF}
- Conoly PCR for E. coli PDF
- Chemical transformation for E. coli PDF
- E. coli Heat-shock transformation PDF
- Golden Gate Assembly using the Echo liquid handler
- Making electrocompetent E. coli* cells PDF
- Gel Electrophoresis PDF
- Gibson Assembly PDF
- Glycerol stocks PDF
- Golden Gate Assembly PDF
- Home-made E. coli competent cells with CaCl2. PDF
- Sequencing PDF
- E. coli transformation with TSS PDF
Lab Notebook
July 15, 2022 :
- Transformation pETDuet- p15
Jul 17, 2022 :
- Liquid cultures of pACYCDuet-1 p17/pCDFDuet-1 p16/pCOLADuet-1 p18
Jul 18, 2022 :
- Antibiotic Resistance Assay
- Miniprep : pACYCDuet-1 p17/pCDFDuet-1 p16/pCOLADuet-1 p18
- Transformation dpB.015, dpB016, ppB.030
- Tranformation pETDuet-p15
Jul 19, 2022:
- Miniprep : pACYCDuet-1 p17/pCDFDuet-1 p16/pCOLADuet-1 p18
- Transformation :ppB.030/ppb.029 ; ppB.028/ppB.029 ; ppB.028/29/30
- Transformation: ppB.030/ppB.0.28
Jul 20, 2022:
- Transformation of p15 in commercial comp cells
- Liquid culture all pDuet( exept pET) + GFP antoine + Moclo DVA et DVE
Jul 26, 2022:
- CIDAR MoClo Vector UPGRADE
- IO rodeo potentiostat, electrode wiring
- Cloning PnasA for B.Subtilis
Jul 28, 2022:
Aug 1, 2022:
- Miniprep: dpB 004, 006, 010, 013, 016, 075
- NanoDrop of minipreps
- First test of potentiostat (success!)
- Cultures on kanamycin plates
Aug 2, 2022 :
- UV view on cultures
- Miniprep (NEW PROTOCOL): dpB 006, pp038
- NanoDrop miniprep dpB006, pp038
- Cloning of dp.012
- Colony PCR + gel v35
Aug 3, 2022 :
- Glycerol stock
- Colony PCR v35 + gel
Aug 4, 2022 :
- Transformation dpB.008 dpB.009 dpB.011
- Miniprep by new protocol 1st part, nanodrop, liquid culture
- Colony PCR
Aug 7, 2022 :
- Miniprep by new protocol 2nd part + results analysis
Aug 9, 2022:
- Sequencing dpB.003,dpB.005,dpB.006,dpB.007,dpB.008, dpB 009, dpB.011, dpB.012, dpB.013
Aug 10, 2022:
- Cloning for dpB 018 & 019 (Act106 & Inv106) from JL
- Miniprep dpB.001, dpB.002, dpB.006, dpB.010, dpB.014
Aug 12, 2022:
- Miniprep dpB 018 & 019 (Act 106 & Inv 106)
Aug 14,2022:
- Miniprep dpB 018 & 019 (Act 106 & Inv 106)
Aug 15, 2022:
- Processor parts building and characterisation
- Test of diff.concentration of inducers for dpB.010
Aug 16, 2022:
- Testing Microscope slides for Hardware
Aug 17, 2022:
- Simple OD Meter
- Miniprep Moclo parts for Echo (pp.048, pp.049,pp.050)
Aug 18, 2022:
- Upgade pDuet with V35, Vector cloning
- Primer dilution and sequencing prep
Aug 19, 2022:
- Plate Reader Inducers Marionettes
Aug 21, 2022:
- Golden Gate pDuet and G Blocks
- Bacteria Electroshock
Aug 22, 2022:
- Plate Reader Assay Inv & Act
- Transformation of b.sub
- Perform lacZ screening assay
- Perfom starch test
- Characterisation of the promotor
- Building all pDuet with the g.block and try different combinaison
to test the processor and the marionettes system with multiple plasmids
Aug 23, 2022:
- building of all dpB in the 3 pDUET by goldengate
- Plate Reader Assay pOxyS
- Get the electroshock 96 well plate as well as the microscope slide ready for experiments
- Ideally get one experiment on Amplitude running
- Get the Uri Alon Library working for experiments (prepare batch culture and clone the library)
Aug 24, 2022:
- Transformation of all pDUET in comp cells
Aug 25, 2022:
- Plate Reader Assay pSoxR
Aug 26,2022:
- Plate Reader assay pOxyS - Higher concentrations
Aug 27, 2022:
- Microscope electroshock
- Subtitoolkit Replica Plate
- Electrical conductivity meter - 555 Timer
Aug 28,2022 :
- Gibson Assembly Debug
- Sequencing pDuets
Aug 29, 2022:
- Microscope electroshock
- Uri Alon Library
Sep 1, 2022:
Sep 2, 2022:
- Output Conductivity Characterization #1
Sep 8, 2022:
- [FACS] Electroshocking bacteria on 96 Well plate
Sep 10, 2022
- Miniprep for Paris_Inv Gibson
- Growth Curve Uri Alon m9
Sep 11, 2022
Sep 13, 2022:
Sep 15, 2022:
- Transformation Partnership Training Constructs
Sep 18, 2022:
- Streak of Paris_Inv and Training constructs
- Gibson Assembly Paris_Inv
- PCR Clean-up
Sep 20, 2022:
- Colony PCR G3-1 and 7 G6 + gel
- [FACS] Electroshocking bacteria on 96 Well plate
Sep 21, 2022:
- Miniprep of STK parts for Echo and Gibson Paris_Inv for seq
- [FACS] Electroshocking bacteria on 96 Well plate
Sep 22, 2022:
Sep 27, 2022:
- Chronoamperometry Paris_Inv - 1 hour
Sep 28, 2022:
- STK 110 plating
- Chronoamperometry Paris_Inv - 8 min
Sep 29, 2022:
- Chronoamperometry - 6 hours and 12 hours
- Pduet Plate reader experiment
Sep 30, 2022:
- Chronoamperometry pOxyS - 6 hours
- Chart characterization
Oct 2, 2022:
- Plate Reader Output Experiment
- Activation Inverter PsoxR
Oct 6, 2022:
- Chronoamperometry - 16 hours
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