Part colletion |
The Paris Bettencourt Electro-Genetic Collection is of a set genetic parts enabling the control and monitoring of gene expression with the specific goal of producing bio-hybrid computers as backbones of the Internet of Living Things. The collection comprises the basic parts we used to develop our constructs, a library of Electro-Responsive promoters we discovered through the joined used of multiple devices from our Hardware Collection and our custom software tools (See the screening experiment for details), a set of composite parts based on our 3 plasmid system enabling Electro-genetic signal processing in live bacterial cultures and a set of 3 compatible vectors for extending the collection with new and compatibles parts. The behaviour of these parts assembled in a 3-plasmid system and performing a simple signal transduction experiment can be seen in our Proof of Concept. |
code | BioBrick | name | type | ||
---|---|---|---|---|---|
1 | ppB.001 | Bba_K4216005 | J23101 | Promoter | |
2 | ppB.004 | Bba_K4216006 | pSoxS | Promoter | |
3 | ppB.006 | Bba_K4217006 | pOxyR | Promoter | |
5 | ppB.007 | Bba_K4216008 | pCin | Promoter | |
6 | ppB.008 | Bba_K4216009 | pLux76B | Promoter | |
7 | ppB.009 | Bba_K4216010 | pTac | Promoter | |
8 | ppB.010 | Bba_K4216011 | pNasA | Promoter | |
9 | ppB.011 | Bba_K4216012 | U2m | RBS | |
10 | ppB.012 | Bba_K4216013 | U4m | RBS | |
11 | ppB.013 | Bba_K4216014 | U6m | RBS | |
12 | ppB.014 | Bba_K4216015 | U8m | RBS | |
13 | ppB.015 | Bba_K4216016 | U1d | RBS | |
14 | ppB.016 | Bba_K42160171 | U2d | RBS | |
15 | ppB.017 | Bba_K4216018 | U3d | RBS | |
16 | ppB.018 | Bba_K4216019 | mScarlet | CDS | |
17 | ppB.019 | Bba_K4216020 | CinR | CDS | |
18 | ppB.020 | Bba_K4216021 | LuxRQ | CDS | |
19 | ppB.021 | Bba_K4216022 | LacI-M | CDS | |
20 | ppB.022 | Bba_K4216023 | Beta-Gal | CDS | |
21 | ppB.023 | Bba_K4216024 | SoxR | CDS | |
22 | ppB.024 | Bba_K4216025 | φX174 | CDS |
Plate number | Well | Name | BioBrick | Effect | ||
---|---|---|---|---|---|---|
1 | AZ_01 | B6 | cueO | Bba_K4216050 | induction | |
2 | AZ_01 | F2 | aroF | Bba_K4216053 | induction | |
3 | AZ_01 | F3 | U66 | Bba_K4216054 | repression | |
4 | AZ_01 | H4 | glcB | Bba_K4216060 | repression | |
5 | AZ_01 | E9 | prmB | Bba_K4216063 | induction | |
6 | AZ_02 | C5 | gltA | Bba_K4216047 | induction | |
7 | AZ_02 | H11 | fumB | Bba_K4216051 | induction | |
8 | AZ_03 | C4 | yhfK | Bba_K4216052 | repression | |
9 | AZ_04 | B10 | zraP | Bba_K4216057 | induction | |
10 | AZ_04 | G7 | prlC | Bba_K4216062 | repression | |
11 | AZ_05 | F3 | U66 | Bba_K4216064 | induction | |
12 | AZ_05 | H3 | crl | Bba_K4216058 | repression | |
13 | AZ_05 | D12 | smtA | Bba_K4216061 | repression | |
14 | AZ_05 | E6 | deoC | Bba_K4216065 | repression | |
15 | AZ_05 | E12 | upp | Bba_K4216049 | induction | |
16 | AZ_05 | D11 | gsk | Bba_K4216055 | repression | |
17 | AZ_01 | F12 | ptsG | Bba_K4216056 | repression | |
18 | AZ_17 | F12 | cspI | Bba_K4216048 | repression | |
19 | AZ_05 | F12 | purM | Bba_K4216059 | repression |
Name | BioBrick | Antibiotic | ||
---|---|---|---|---|
1 | pACYDuet_Bettencourt | Bba_K4216043 | Chloramphenicol | |
2 | pCDFDuet_Bettencourt | Bba_K4216044 | Spectinomycine | |
3 | pCOLADuet_Bettencourt | Bba_K4216045 | Kanamycin |
Name | BioBrick | Construct | ||
---|---|---|---|---|
1 | dpB.002 | Bba_K4216027 | pSoxR - mScarlet | |
2 | dpB.003 | Bba_K4216028 | pSoxR - CinR | |
3 | dpB.004 | Bba_K4216029 | pOxyS - mScarlet | |
4 | dpB.005 | Bba_K4216030 | pOxyS - CinR | |
5 | dpB.006 | Bba_K4216031 | pNasA - mScarlet | |
6 | dpB.007 | Bba_K4216032 | pNasA - CinR | |
7 | dpB.008 | Bba_K4216033 | J23101 - CinR | |
8 | dpB.009 | Bba_K4216034 | J23101 - LacI - pTac - CinR |
Name | BioBrick | Construct | ||
---|---|---|---|---|
1 | dpB.010 | Bba_K4216035 | PCin - mScarlet | |
2 | dpB.011 | Bba_K4216036 | pCin - LuxRQ | |
3 | dpB.012 | Bba_K4216037 | pCin - LacI - pTac - LuxRQ |
Name | BioBrick | Function | ||
---|---|---|---|---|
1 | dpB.013 | Bba_K4216038 | pLux78B - mScarlet | |
2 | dpB.014 | Bba_K4216039 | pLux78B - φX174 (Lysis) | |
3 | dpB.015 | Bba_K4216040 | pLux78B - LacZ |