Part colletion

About

The Paris Bettencourt Electro-Genetic Collection is of a set genetic parts enabling the control and monitoring of gene expression with the specific goal of producing bio-hybrid computers as backbones of the Internet of Living Things.

The collection comprises the basic parts we used to develop our constructs, a library of Electro-Responsive promoters we discovered through the joined used of multiple devices from our Hardware Collection and our custom software tools (See the screening experiment for details), a set of composite parts based on our 3 plasmid system enabling Electro-genetic signal processing in live bacterial cultures and a set of 3 compatible vectors for extending the collection with new and compatibles parts.

The behaviour of these parts assembled in a 3-plasmid system and performing a simple signal transduction experiment can be seen in our Proof of Concept.

Lexicon

Input:
Refers to devices or parts that can transcribe an electrical signal into the production of a protein
Processor:
Refers to information processing circuits which input is pCin and wich output the protein LuxR (receiving signals from the inputs and passing on transformed signals to the output)
Outputs:
Refers to circuits which input is pLux and which output an electronically readable signal
Electro-regulated promoters:
Refers to a set of 19 promoter with Electro-Responsive property we discovered through our screening experiment
Vectors:
A set of 3 compatible vectors to add new Inputs, Processors and Outputs to the collection


Basic Parts

code BioBrick name type
1 ppB.001 Bba_K4216005 J23101 Promoter
2 ppB.004 Bba_K4216006 pSoxS Promoter
3 ppB.006 Bba_K4217006 pOxyR Promoter
5 ppB.007 Bba_K4216008 pCin Promoter
6 ppB.008 Bba_K4216009 pLux76B Promoter
7 ppB.009 Bba_K4216010 pTac Promoter
8 ppB.010 Bba_K4216011 pNasA Promoter
9 ppB.011 Bba_K4216012 U2m RBS
10 ppB.012 Bba_K4216013 U4m RBS
11 ppB.013 Bba_K4216014 U6m RBS
12 ppB.014 Bba_K4216015 U8m RBS
13 ppB.015 Bba_K4216016 U1d RBS
14 ppB.016 Bba_K42160171 U2d RBS
15 ppB.017 Bba_K4216018 U3d RBS
16 ppB.018 Bba_K4216019 mScarlet CDS
17 ppB.019 Bba_K4216020 CinR CDS
18 ppB.020 Bba_K4216021 LuxRQ CDS
19 ppB.021 Bba_K4216022 LacI-M CDS
20 ppB.022 Bba_K4216023 Beta-Gal CDS
21 ppB.023 Bba_K4216024 SoxR CDS
22 ppB.024 Bba_K4216025 φX174 CDS

Electro-regulated promoters

Plate number Well Name BioBrick Effect
1 AZ_01 B6 cueO Bba_K4216050 induction
2 AZ_01 F2 aroF Bba_K4216053 induction
3 AZ_01 F3 U66 Bba_K4216054 repression
4 AZ_01 H4 glcB Bba_K4216060 repression
5 AZ_01 E9 prmB Bba_K4216063 induction
6 AZ_02 C5 gltA Bba_K4216047 induction
7 AZ_02 H11 fumB Bba_K4216051 induction
8 AZ_03 C4 yhfK Bba_K4216052 repression
9 AZ_04 B10 zraP Bba_K4216057 induction
10 AZ_04 G7 prlC Bba_K4216062 repression
11 AZ_05 F3 U66 Bba_K4216064 induction
12 AZ_05 H3 crl Bba_K4216058 repression
13 AZ_05 D12 smtA Bba_K4216061 repression
14 AZ_05 E6 deoC Bba_K4216065 repression
15 AZ_05 E12 upp Bba_K4216049 induction
16 AZ_05 D11 gsk Bba_K4216055 repression
17 AZ_01 F12 ptsG Bba_K4216056 repression
18 AZ_17 F12 cspI Bba_K4216048 repression
19 AZ_05 F12 purM Bba_K4216059 repression

Vectors

Name BioBrick Antibiotic
1 pACYDuet_Bettencourt Bba_K4216043 Chloramphenicol
2 pCDFDuet_Bettencourt Bba_K4216044 Spectinomycine
3 pCOLADuet_Bettencourt Bba_K4216045 Kanamycin


Inputs

Name BioBrick Construct
1 dpB.002 Bba_K4216027 pSoxR - mScarlet
2 dpB.003 Bba_K4216028 pSoxR - CinR
3 dpB.004 Bba_K4216029 pOxyS - mScarlet
4 dpB.005 Bba_K4216030 pOxyS - CinR
5 dpB.006 Bba_K4216031 pNasA - mScarlet
6 dpB.007 Bba_K4216032 pNasA - CinR
7 dpB.008 Bba_K4216033 J23101 - CinR
8 dpB.009 Bba_K4216034 J23101 - LacI - pTac - CinR

processors

Name BioBrick Construct
1 dpB.010 Bba_K4216035 PCin - mScarlet
2 dpB.011 Bba_K4216036 pCin - LuxRQ
3 dpB.012 Bba_K4216037 pCin - LacI - pTac - LuxRQ

outputs

Name BioBrick Function
1 dpB.013 Bba_K4216038 pLux78B - mScarlet
2 dpB.014 Bba_K4216039 pLux78B - φX174 (Lysis)
3 dpB.015 Bba_K4216040 pLux78B - LacZ