We approached academics and industrialists about the obstacles they encounter when producing and purifying proteins for our Integrated Human Practices. We sought information about chassis and the proteins they make. All of academicians stated that they would spend money on a better plasmid for gene expression and would re-optimize the downstream procedures if necessary. We have taken into account the industrialists' concerns about performance of the plasmid in terms of purity and yields in a scaled up condition and the protection of their intellectual property rights in our future pipeline and implementation process.
We have followed the 3R Models of Reflections, Responsibility and Responsiveness.
We understood on how our project could potentially impact the industry as well as the academicians. This enabled us to better appreciate our project and connect them with real world applications. We understood the safety aspects of our project. The next set of research to be planned and done to provide a better impact as a responsible researcher. We have been continuously responding to the suggestions received through our Human Practices. This reflects in both of our Engineering Cycles. We were initially considering more complex things such as use of sRNAs, inducible 5'UTR systems. However, first we decide to start and prove the basic for this year's iGEM project as given to understand by our Human Practices. And, we achieved our goal!
We have held conversations about our project from the beginning with experts and stakeholders from different walks of life. Their inputs have impacted our Design, Build, Test, Learn and goals for Proposed Implementation. As you click on the tiles, the details of each interaction will be elaborated upon.
