Part Collection

We have used the BBa_B0030 RBS downstream of our 5’UTR and the promoter BBa_J23100 in the Dh5alpha and BL21(DE3) strains of E coli. We made the monomeric RFP BBa_E1010 as our reporter gene. Our parts give a range of expressions as described below. iGEM teams or any other users can use our parts. We advise them to use BBa_K4303026 in conjunction with the RBS BBa_0030 to receive an enhanced expression by over 30%.


Parts Added to the Registry


We have documented all our literature search on our Wiki Page. We have also added the parts that we have used and made them openly available for any team. We have updated the code of BBrickIt and it is open source for anyone to use and available on our Software Page. We have also made our protocols and project models available for any present or future iGEM teams to use. We have also made Education and Communication materials available for anyone to use. It is encouraged that people follow or make sure that the Sustainability practices and procedures that we have followed at the lab level are followed.


Links Name Symbol Comparative Performance in DH5alpha strain Comparative Performance in BL21(DE3) strain Sequence
http://parts.igem.org/Part:BBa_K4303015 BBa_K4303015 ompA 0.8865 0.6357 CGCCAGGGGTGCTCGGCATAAGCCGAAGATATCGGTAGAGTTAATATTGAGCAGATCCCCCGGTGAAGGATTTAACCGTGTTATCTCGTTGGAGATATTCATGGCGTATTTTGGATGATAACGAGGCGCAAAAA
http://parts.igem.org/Part:BBa_K4303016 BBa_K4303016 mopB 0.3658 0.2928 ACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCA
http://parts.igem.org/Part:BBa_K4303017 BBa_K4303017 CspF 1.1638 0.6922 GGAATTTTT
http://parts.igem.org/Part:BBa_K4303007 BBa_K4303007 CspH 0.2438 0.4194 GCAGCGCAGTATTTTCAGTAAGGAATTTGT
http://parts.igem.org/Part:BBa_K4303008 BBa_K4303008 syn2D 0.0831 0.1795 AGCCAGGGGTGCTCGGCATAAGCCGAAGATATCGGTAGAGTTAATATTGAGCAGATCCCCCGGTCGTATTTTGGATGACTAGAGAAAGAGGAGAAA
http://parts.igem.org/Part:BBa_K4303009 BBa_K4303009 syn2e 0.0160 0.0263 AACGTCGACTTATCTCGAGTGAGATATTGTTGACGGTTACCGTATTTTCTAGAGAAAGAGGAGAAA
http://parts.igem.org/Part:BBa_K4303011 BBa_K4303011 syn2h 0.0948 0.1602 AACGTCGACTTATCTCGAGACTGCAGTTCAATAGAGATATTGTTGACGGTACCGTATTTTCGTATTTTGGATGACTAGAGAAAGAGGAGAAA
http://parts.igem.org/Part:BBa_K4303012 BBa_K4303012 U9 0.1926 0.3025 GGTACATTAGAAAGGAGGAATGTACC
http://parts.igem.org/Part:BBa_K4303013 BBa_K4303013 U10 0.1765 0.2911 GTACATTAGAAAGGAGGAATGTACC
http://parts.igem.org/Part:BBa_K4303014 BBa_K4303014 U13 0.0816 0.1022 GTGATTAGAAAGGAGGAATGTACA
http://parts.igem.org/Part:BBa_K4303006 BBa_K4303006 B1016 0.6418 1.1264 ACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGC
http://parts.igem.org/Part:BBa_K4303005 BBa_K4303005 yeiG 1.0177 1.1571 ACAACACAACAAGGAGCCACGC
http://parts.igem.org/Part:BBa_K4303003 BBa_K4303003 ynfA 0.8817 1.2123 ATACATGATGTCAATAAGAGACAAAG
http://parts.igem.org/Part:BBa_K4303002 BBa_K4303002 ahpC 0.9372 1.1641 ATCGAAAACACGGAGGAAGTATAG
http://parts.igem.org/Part:BBa_K4303001 BBa_K4303001 yhjT 0.8341 0.9736 AGACAAAATGGTGAC
http://parts.igem.org/Part:BBa_K4303000 BBa_K4303000 sfsA 1.1392 1.0330 GCGCTAACGCAATAACAAGGATTGTCGCA
http://parts.igem.org/Part:BBa_K4303018 BBa_K4303018 SDM17 1.0323 0.2794 GGGATTTTT
http://parts.igem.org/Part:BBa_K4303019 BBa_K4303019 SDM2 0.9995 0.6127 GGAGTTTTT
http://parts.igem.org/Part:BBa_K4303020 BBa_K4303020 SDM3 0.9501 0.3737 GGAATATTT
http://parts.igem.org/Part:BBa_K4303021 BBa_K4303021 SDM23 1.1571 0.5179 GGAATTTTA
http://parts.igem.org/Part:BBa_K4303022 BBa_K4303022 SDM5 1.1293 0.5208 GGAATTTTC
http://parts.igem.org/Part:BBa_K4303023 BBa_K4303023 SDM19 1.0667 0.6004 GTAATTTTT
http://parts.igem.org/Part:BBa_K4303024 BBa_K4303024 SDM10 1.2081 0.4340 GGAAGTTTT
http://parts.igem.org/Part:BBa_K4303026 BBa_K4303026 SDM27 0.9891 0.6213 GGAATTTCT
http://parts.igem.org/Part:BBa_K4303027 BBa_K4303027 SDM26 1.0168 0.4426 GGACTTTTT
http://parts.igem.org/Part:BBa_K4303028 BBa_K4303028 SDM9 0.9329 0.7207 GGAACTTTT
http://parts.igem.org/Part:BBa_K4303029 BBa_K4303029 katE 0.1053 0.1374 TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCA
http://parts.igem.org/Part:BBa_K4303030 BBa_K4303030 Syn2F 0.5468 0.3400 AACGTCGACTTATCTCGAGACTGCAGTTCAATAGAGATATTGTTGACGGTACCGTATTTTCTAGAGAAAGAGGAGAAA
http://parts.igem.org/Part:BBa_K4303031 BBa_K4303031 wrbA 0.1963 0.2703 AGTGGTAGCGAATCGCTACGGAATAGAGATAACACGAGGAGTGGTTAGAA