We have used the BBa_B0030 RBS downstream of our 5’UTR and the promoter BBa_J23100 in the Dh5alpha and BL21(DE3) strains of E coli. We made the monomeric RFP BBa_E1010 as our reporter gene. Our parts give a range of expressions as described below. iGEM teams or any other users can use our parts. We advise them to use BBa_K4303026 in conjunction with the RBS BBa_0030 to receive an enhanced expression by over 30%.
We have documented all our literature search on our Wiki Page. We have also added the parts that we have used and made them openly available for any team. We have updated the code of BBrickIt and it is open source for anyone to use and available on our Software Page. We have also made our protocols and project models available for any present or future iGEM teams to use. We have also made Education and Communication materials available for anyone to use. It is encouraged that people follow or make sure that the Sustainability practices and procedures that we have followed at the lab level are followed.
| Links | Name | Symbol | Comparative Performance in DH5alpha strain | Comparative Performance in BL21(DE3) strain | Sequence |
| http://parts.igem.org/Part:BBa_K4303015 | BBa_K4303015 | ompA | 0.8865 | 0.6357 | CGCCAGGGGTGCTCGGCATAAGCCGAAGATATCGGTAGAGTTAATATTGAGCAGATCCCCCGGTGAAGGATTTAACCGTGTTATCTCGTTGGAGATATTCATGGCGTATTTTGGATGATAACGAGGCGCAAAAA |
| http://parts.igem.org/Part:BBa_K4303016 | BBa_K4303016 | mopB | 0.3658 | 0.2928 | ACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCA |
| http://parts.igem.org/Part:BBa_K4303017 | BBa_K4303017 | CspF | 1.1638 | 0.6922 | GGAATTTTT |
| http://parts.igem.org/Part:BBa_K4303007 | BBa_K4303007 | CspH | 0.2438 | 0.4194 | GCAGCGCAGTATTTTCAGTAAGGAATTTGT |
| http://parts.igem.org/Part:BBa_K4303008 | BBa_K4303008 | syn2D | 0.0831 | 0.1795 | AGCCAGGGGTGCTCGGCATAAGCCGAAGATATCGGTAGAGTTAATATTGAGCAGATCCCCCGGTCGTATTTTGGATGACTAGAGAAAGAGGAGAAA |
| http://parts.igem.org/Part:BBa_K4303009 | BBa_K4303009 | syn2e | 0.0160 | 0.0263 | AACGTCGACTTATCTCGAGTGAGATATTGTTGACGGTTACCGTATTTTCTAGAGAAAGAGGAGAAA |
| http://parts.igem.org/Part:BBa_K4303011 | BBa_K4303011 | syn2h | 0.0948 | 0.1602 | AACGTCGACTTATCTCGAGACTGCAGTTCAATAGAGATATTGTTGACGGTACCGTATTTTCGTATTTTGGATGACTAGAGAAAGAGGAGAAA |
| http://parts.igem.org/Part:BBa_K4303012 | BBa_K4303012 | U9 | 0.1926 | 0.3025 | GGTACATTAGAAAGGAGGAATGTACC |
| http://parts.igem.org/Part:BBa_K4303013 | BBa_K4303013 | U10 | 0.1765 | 0.2911 | GTACATTAGAAAGGAGGAATGTACC |
| http://parts.igem.org/Part:BBa_K4303014 | BBa_K4303014 | U13 | 0.0816 | 0.1022 | GTGATTAGAAAGGAGGAATGTACA |
| http://parts.igem.org/Part:BBa_K4303006 | BBa_K4303006 | B1016 | 0.6418 | 1.1264 | ACTTTTCTTTGCATAGATTGACTCAGAAAAACGTTTAAGGGTGGGTGGC |
| http://parts.igem.org/Part:BBa_K4303005 | BBa_K4303005 | yeiG | 1.0177 | 1.1571 | ACAACACAACAAGGAGCCACGC |
| http://parts.igem.org/Part:BBa_K4303003 | BBa_K4303003 | ynfA | 0.8817 | 1.2123 | ATACATGATGTCAATAAGAGACAAAG |
| http://parts.igem.org/Part:BBa_K4303002 | BBa_K4303002 | ahpC | 0.9372 | 1.1641 | ATCGAAAACACGGAGGAAGTATAG |
| http://parts.igem.org/Part:BBa_K4303001 | BBa_K4303001 | yhjT | 0.8341 | 0.9736 | AGACAAAATGGTGAC |
| http://parts.igem.org/Part:BBa_K4303000 | BBa_K4303000 | sfsA | 1.1392 | 1.0330 | GCGCTAACGCAATAACAAGGATTGTCGCA |
| http://parts.igem.org/Part:BBa_K4303018 | BBa_K4303018 | SDM17 | 1.0323 | 0.2794 | GGGATTTTT |
| http://parts.igem.org/Part:BBa_K4303019 | BBa_K4303019 | SDM2 | 0.9995 | 0.6127 | GGAGTTTTT |
| http://parts.igem.org/Part:BBa_K4303020 | BBa_K4303020 | SDM3 | 0.9501 | 0.3737 | GGAATATTT |
| http://parts.igem.org/Part:BBa_K4303021 | BBa_K4303021 | SDM23 | 1.1571 | 0.5179 | GGAATTTTA |
| http://parts.igem.org/Part:BBa_K4303022 | BBa_K4303022 | SDM5 | 1.1293 | 0.5208 | GGAATTTTC |
| http://parts.igem.org/Part:BBa_K4303023 | BBa_K4303023 | SDM19 | 1.0667 | 0.6004 | GTAATTTTT |
| http://parts.igem.org/Part:BBa_K4303024 | BBa_K4303024 | SDM10 | 1.2081 | 0.4340 | GGAAGTTTT |
| http://parts.igem.org/Part:BBa_K4303026 | BBa_K4303026 | SDM27 | 0.9891 | 0.6213 | GGAATTTCT |
| http://parts.igem.org/Part:BBa_K4303027 | BBa_K4303027 | SDM26 | 1.0168 | 0.4426 | GGACTTTTT |
| http://parts.igem.org/Part:BBa_K4303028 | BBa_K4303028 | SDM9 | 0.9329 | 0.7207 | GGAACTTTT |
| http://parts.igem.org/Part:BBa_K4303029 | BBa_K4303029 | katE | 0.1053 | 0.1374 | TTTTTTGACCAAAACAGCGGCCCTTTCAGTAATAAATTAAGGAGACGAGTTCA |
| http://parts.igem.org/Part:BBa_K4303030 | BBa_K4303030 | Syn2F | 0.5468 | 0.3400 | AACGTCGACTTATCTCGAGACTGCAGTTCAATAGAGATATTGTTGACGGTACCGTATTTTCTAGAGAAAGAGGAGAAA |
| http://parts.igem.org/Part:BBa_K4303031 | BBa_K4303031 | wrbA | 0.1963 | 0.2703 | AGTGGTAGCGAATCGCTACGGAATAGAGATAACACGAGGAGTGGTTAGAA |