Primers and gBlocks

ID	Contents	Sequence 5' --> 3'	Tm ( C )
iGEM 001	IL-10 (PLS13) plasmid Sequencing primer FW	CAGGGTTATTGTCTCATGAGCGGATAC	61
iGEM 002	IL-10 (PLS13) plasmid Sequencing primer RV	GGAGGTGGGGAGCAGGAGAACTCC	65
iGEM 003	IL-10 gBlock PCR primers FW	CGATATCTAGAATGCACTCAAGCGCTCTC	61
iGEM 004	IL-10 gBlock PCR primers RV	CGATGGGGTCCCTTAGTTGCGGATCTTC	61
iGEM 005	PR3 gblock PCR fw	GCGTACGTAGGGATCCATCGTGGG	64
iGEM 006	PR3 gblock PCR rv	CGCTAGCATGAATTCCCTCAATGTTGAT	61
iGEM 007	pLeo619 Seq FW	GCAAAAAGCTTCGAATCGCGCTAGC	64
iGEM 008	pLeo619 Seq RV	ACCTACTCAGACAATGCGATGCAATTTC	61
iGEM 009	PLS15 Seq FW	GCTAACTAGAGAACCCACTGCTTACTG	60
iGEM 010	PLS15 Seq MD	CGCCCAACATCTGCCTAGATCGGCT	66
iGEM 011	PLS15 Seq RV	CTGGCAACTAGAAGGCACAGTCGAGG	64
iGEM 012	PLS13 Seq FW	CAGGGTTATTGTCTCATGAGCGGATAC	61
iGEM 013	PLS13 Seq RV	GACGGGACACTCAGGGAGCAGTGG	66
iGEM 014	pLeo-x-P3 FW	GCAAAAAGCTTCGAATCGCGCTAGCG	66
iGEM 015	pLeo-x-P3 RV	GGAGTGGCACCTTCCAGGGTCAAGG	66
iGEM 016	Gibson Fragment.FOR	GATCAACGGGGGACGGATCCATCGTGGGAGGGC	71.6
iGEM 017	Gibson Fragment.REV	GATCCACTACGAATGAATTCCCTCAATGTTGATCGAATCC	63.5
iGEM 018	Gibson Vector.FOR	GCTTCATGCCCTCCCACGATGGATCCGTCCCCCGTTGA	72.4
iGEM 019	Gibson Vector.REV	GCTTCATGCCCTCCCACGATGGATCCGTCCCCCGTTGATCCA	72.8
iGEM 020	PR3 gblock PCR fw, KflI	GCTGTGGGTCCCTGGATCAACGGGGGACGGATCCATCGTGGGAGGGCA	71
iGEM 021	Part Improvent + UTR PCR FW	GATTTCTGGAATTCGCGGCCGCTTCTAGAG	66
iGEM 022	Part Improvent + UTR PCR RV	CTGCAGCGGCCGCTACTAGTATATAAACG	64
iGEM 023	Part Improvent + UTR Sequencing FW	GACATTAACCTATAAAAATAGGCGTATCACGAGGC	62
iGEM 024	Part Improvent + UTR Sequencing RV	GCAGGGTGGTGACACCTTGCCCTTTTTTGC	68
iGEM 025	PR3 gblock PCR rv, HA-tag	CGCTAGCATGAATTCAGCGTAATCTGGAACATCGTATGGGTACCTC	69
iGEM 026	PR3 + HA tag sequencing primer, fw (middle primer for sequencing)	GGTGACTCTGGCGGGCCCCTTATTTGTGACGGG	64
iGEM 027	Control primers P.I., fw	CCACCTGACGTCTAAGAA	52
iGEM 028	Control primers P.I., rv	GTATTACCGCCTTTGAGT	51

ID	Sequence 5' --> 3'
IL-10 gBlock	CGATATCTAGAATGCACTCAAGCGCTCTCCTTTGCTGTCTTGTTCTCCTCACAGGAGTACGCGCTTCACCCGGACAAGGCACACAGTCTGAAAATTCATGCACACATTTTCCCGGCAACCTCCCAAATATGCTTAGGGATCTCCGCGACGCGTTTAGTAGAGTAAAGACGTTCTTTCAAATGAAGGACCAATTGGACAATTTGCTCTTGAAGGAGAGCCTTCTCGAAGATTTCAAGGGTTACCTTGGTTGTCAAGCCCTTTCTGAGATGATCCAATTCTACTTGGAGGAAGTAATGCCACAAGCGGAAAACCAAGATCCTGATATTAAAGCTCATGTAAATTCTCTGGGAGAGAACCTCAAGACCTTGAGGCTCCGCTTGCGACGGTGCCACAGATTCTTGCCTTGCGAAAATAAGAGTAAAGCCGTAGAGCAAGTCAAAAATGCGTTTAATAAGTTGCAAGAGAAGGGAATATATAAGGCGATGTCCGAGTTTGACATATTCATTAACTACATTGAGGCTTATATGACAATGAAGATCCGCAACTAAGGGACCCCATCG
PR3 gBlock	GCGTACGTAGGGATCCATCGTGGGAGGGCATGAAGCCCAACCGCACAGCAGGCCCTATATGGCGAGCCTTCAAATGCGCGGCAATCCAGGTAGCCATTTTTGTGGTGGGACACTTATCCATCCCTCTTTCGTGCTCACTGCAGCGCATTGCCTGCGAGACATCCCACAACGCCTTGTGAATGTAGTGCTGGGGGCTCACAATGTACGAACTCAAGAGCCCACACAACAGCACTTTTCTGTCGCGCAGGTGTTCCTTAACAATTATGACGCTGAAAACAAACTCAATGATGTCCTCCTTATCCAACTTAGCAGTCCCGCAAATTTGTCCGCCTCTGTGGCTACTGTTCAACTGCCGCAACAGGATCAGCCAGTTCCCCACGGGACTCAATGTCTTGCAATGGGCTGGGGCAGAGTCGGGGCCCACGATCCCCCTGCGCAAGTGCTGCAAGAACTTAACGTAACTGTTGTTACATTCTTTTGCAGACCTCATAACATCTGCACCTTCGTGCCACGACGCAAGGCTGGCATTTGTTTCGGTGACTCTGGCGGGCCCCTTATTTGTGACGGGATAATACAAGGAATTGACAGTTTCGTTATATGGGGATGTGCGACACGCCTGTTTCCCGACTTCTTTACGCGGGTCGCACTCTATGTCGATTGGATTCGATCAACATTGAGGGAATTCATGCTAGCG
PR3+HA-tag gBlock	GCTGTGGGTCCCTGGATCAACGGGGGACGGATCCATCGTGGGAGGGCATGAAGCCCAACCGCACAGCAGGCCCTATATGGCGAGCCTTCAAATGCGCGGCAATCCAGGTAGCCATTTTTGTGGTGGGACACTTATCCATCCCTCTTTCGTGCTCACTGCAGCGCATTGCCTGCGAGACATCCCACAACGCCTTGTGAATGTAGTGCTGGGGGCTCACAATGTACGAACTCAAGAGCCCACACAACAGCACTTTTCTGTCGCGCAGGTGTTCCTTAACAATTATGACGCTGAAAACAAACTCAATGATGTCCTCCTTATCCAACTTAGCAGTCCCGCAAATTTGTCCGCCTCTGTGGCTACTGTTCAACTGCCGCAACAGGATCAGCCAGTTCCCCACGGGACTCAATGTCTTGCAATGGGCTGGGGCAGAGTCGGGGCCCACGATCCCCCTGCGCAAGTGCTGCAAGAACTTAACGTAACTGTTGTTACATTCTTTTGCAGACCTCATAACATCTGCACCTTCGTGCCACGACGCAAGGCTGGCATTTGTTTCGGTGACTCTGGCGGGCCCCTTATTTGTGACGGGATAATACAAGGAATTGACAGTTTCGTTATATGGGGATGTGCGACACGCCTGTTTCCCGACTTCTTTACGCGGGTCGCACTCTATGTCGATTGGATTCGATCAACATTGAGGTACCCATACGATGTTCCAGATTACGCTGAATTCATGCTAGCG
GFP + UTR gBlock	GATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAGATACTAGAGAATAATTTTGTTTAACTTTAAAAAAAAAAAAGAAGGAGAATAATCTTACTAGATGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTACCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAAGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCACGCATGGTATGGATGAACTGTACAAATGATGATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCA
GFP gBlock	GATTTCTGGAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAGATACTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGCGAAGAGCTGTTCACTGGTGTCGTCCCTATTCTGGTGGAACTGGATGGTGATGTCAACGGTCATAAGTTTTCCGTGCGTGGCGAGGGTGAAGGTGACGCAACTAATGGTAAACTGACGCTGAAGTTCATCTGTACTACTGGTAAACTGCCGGTACCTTGGCCGACTCTGGTAACGACGCTGACTTATGGTGTTCAGTGCTTTGCTCGTTATCCGGACCATATGAAGCAGCATGACTTCTTCAAGTCCGCCATGCCGGAAGGCTATGTGCAGGAACGCACGATTTCCTTTAAGGATGACGGCACGTACAAAACGCGTGCGGAAGTGAAATTTGAAGGCGATACCCTGGTAAACCGCATTGAGCTGAAAGGCATTGACTTTAAAGAAGACGGCAATATCCTGGGCCATAAGCTGGAATACAATTTTAACAGCCACAATGTTTACATCACCGCCGATAAACAAAAAAATGGCATTAAAGCGAATTTTAAAATTCGCCACAACGTGGAGGATGGCAGCGTGCAGCTGGCTGATCACTACCAGCAAAACACTCCAATCGGTGATGGTCCTGTTCTGCTGCCAGACAATCACTATCTGAGCACGCAAAGCGTTCTGTCTAAAGATCCGAACGAGAAACGCGATCATATGGTTCTGCTGGAGTTCGTAACCGCAGCGGGCATCACGCATGGTATGGATGAACTGTACAAATGATGATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCA