Why do people from plateau or prairie need your project?
How to make sure that this method can be applied to everyone there? Wouldn’t it be difficult to spread the information and the knowledge?
Will it cost more than current diagnostic method? Will the new method cause a heavier economic burden for patients?
What is the limitataion of detection?
Our test is compared to existing tests (imaging, immunology, tissue biopsy...). The advantage of?
What is the difference between detecting Echinococcus cfDNA and covid19 nucleic acid detection, and what is the difficulty?
First of all, echinococcosis has been bothering residents who are living with cattle and sheep for hundreds of years, although the situation has become better recent years through health publicity and health education along with the efforts made by the government, there are still lots of people beyond their reach are in great danger of getting infected. Secondly, residents there usually are relatively poor and have a relatively insufficient medical condition for in time diagnosis and proper treatment. The biggest they’re facing right now is that going to hospital and visiting doctors are quite hard for them. Therefore, to solve their problems in the way we are able to, we want to give residents there a more convenient way to detect the infection.
We have interviewed professor from the School of Public Health and local residents and doctors, according to them, the best way to spread information about parasitic diseases and information about our project is to contact a middleman first and let the middleman to speak to the residents, who is more familiar with living habits of the residents and more trustworthy for residents. Another thing we can do is that we can contact local clinics, where residents take their basic medical care, make the information and the new method a part of their daily life instead of lectures given by us.
Advantages of our project compared with current diagnostic method are more than the cost. Current methods for echinococcosis diagnosis are imaging diagnosis, antibody tests and biopsy. All these methods requires expensive equipments and skilled personnel. Therefore, these methods are not heap to be used. Our project can achieve the detection with a colloidal gold test paper and a small amount of peripheral blood provided by the patient, the cost of detection is way below current tests.Furthermore,besides cheapness and convenience, the detection of cfDNA in blood can provide a precise evidence for diagnosis without invasive tests, the risk of strong immune reaction, infection and the pain of patients are highly reduced. The economic and physical burden for patients will be minimized.
Our experiments show that the limatation of detection is 10 copies per microliter.
cfDNA can be extracted only by taking blood. No equipment or complicated operation is needed to test cfDNA.
Echinococcus infection produces cysts, which are also the source of the hydatid name, and the insect body is present in these dense cysts. After insect body death or cell apoptosis, DNA is released. But the DNA released at this time is not genomic DNA, at which time the DNA has broken. Because the hydatid cysts is relatively dense, only small fragments of DNA can pass through and be released into the blood, which is also a difficulty in our diagnosis.So our solution is to simultaneously detect multi-targets, design multi-primer pairs for RPA amplification and multi-sgRNA-guided CRISPR Cas12a detection, in order to improve the true positive rate of detection.
