1.Real Sample
To simulate the expected working conditions, the actual patient samples were used to perform mixed detection in the presence of blood samples to verify the detection rate of the kit.
2.Further Design
A one-pot reaction unit containing improved RPA system and CRISPR-Cas12a system was designed. Facilitate transportation, storage and use, and better adapt to the expected working environment of the project team.
3.Deeper Sequencing
Deeper sequencing, more primers without interaction were designed to further improve the coverage and detection rating improved RPA system and CRISPR-Cas12a system was designed. Facilitate transportation, storage and use, and better adapt to the expected working environment of the project team.
4.Cost Control
Although the cost of reagents in the laboratory is more reasonable, our aim is to apply our results to more remote pastoral areas, and cost control becomes an urgent issue to address. The next step is to try to efficiently express all the proteins and nucleic acid products used in the project to reduce the cost.
