| BBA_K118011
| Regulatory |
This is the promoter for the Escherichia coli JM109 cstA gene. It includes the CRP-binding
site and the RNA polymerase-binding site. Low glucose concentration results in increased
activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound
form, is able to associate with the promoter and promote transcription of the downstream
gene. (cstA encodes the carbon starvation protein.) |
Andrew Hall iGEM08_Edinburgh |
Escherichia coli |
131 |
| BBa_B0030
| Regulatory |
Strong RBS based on Ron Weiss thesis |
Vinay S |
Escherichia coli |
15 |
| BBa_B0015
| Regulatory |
BBa_B0015 is a composite terminator made by joining 2 other terminators, one derived from E.
coli (BBa_B0010) and the other from the T7 phage (BBa_B0012). Unlike what one can guess from
the name and origin, the E. coli terminator can terminate transcription by T7 RNA
polymerase, but not the T7 TE terminator. Indeed, BBa_B0010 is the E. coli rrnB T1
terminator which was shown to be an efficient terminator for the E. coli RNA polymerase, but
also for the phage SP6 and T7 RNA polymerases through two different mechanism: one involving
an upstream hairpin structure and the other one a downstream sequence-specific signal [1–3].
However, the T7 TE terminator (BBa_B0012) is located at the end of the T7 DNA ligase gene
which is in the early region of bacteriophage T7 genome [4]. This terminator is an efficient
one for the E. coli RNA polymerase, but not for the T7 RNA polymerase. |
Reshma Shetty iGEM20_Paris
|
Escherichia coli |
129 |
| BBa_R1051
| Regulatory |
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter
has two two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in
repression of transcription. The specific sequence used here is based on the cI repressible
promoter used in the Elowitz repressilator |
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
Drew Endy, iGEM20_UTexas |
Escherichia coli |
49 |
| BBa_K118022
| Coding cex |
The cellulolytic bacterium Cellulomonas fimi uses an exoglucanase (from cex, accession
M15824) along with 3 endoglucanases in the degradation of cellulose into cellobiose, before
use B-glucosidase to catalyse the conversion of cellobiose to D-glucose. |
Andrew Hall, iGEM08_Edinburgh |
Cellulomonas fimi |
1352 |
| BBa_K118023
| Coding cen |
The cellulolytic bacterium Cellulomonas fimi uses 3 endoglucanases (including CenA,
accession M15823) and an exoglucanase in the degradation of cellulose into cellobiose,
before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose. |
Andrew Hall, iGEM08_Edinburgh |
Cellulomonas fimi |
1460 |
| BBa_K4368002
| Basic bglX |
BglX encodes for the β-glucosidase gene of Escherichia coli. This enzyme is responsible of
the degradation of cellulose working coordinated with the genes cenA and cex. This complex
is known as CAZymes. In detail, bglX degradates the cellobiose formed by cenA and cex and
transformed it into glucose. This basic part only corresponds to the coding sequence of the
gene, no RBS or terminator is attached.
|
Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez, Group: iGEM22_UMA_MALAGA
|
Escherichia coli |
2301 |
| BBa_K4368003
| Coding bglX |
BglX encodes for the β-glucosidase gene of Escherichia coli (BBa_K4368002). This enzyme is
responsible of the degradation of cellulose working coordinated with the genes cenA and cex.
In addition, this part includes the composition used by the team, which includes a strong
rbs (BBa_B0030), a double terminator (BBa_B0015) as well as a promoter inducible by glucose
concentration (BBa_K118011). The gene has been placed under the control of this promoter to
build the glucose concentration-based gene regulatory circuit that integrates all our parts.
|
Carmen Jimenez Amores, Alonso Molina Calvo, Paola Morales Gonzalez, Group: iGEM22_UMA_MALAGA
|
Escherichia coli |
2598 |
| BBa_K118016
| Coding glgC |
This is the coding sequence of glgC (ADP-glucose pyrophosphorylase) from Escherichia coli
JM109 with the substitution G336D. This mutation is known to cause increased activity of
ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate
(FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. |
Andrew Hall, iGEM08_Edinburgh
|
Escherichia coli |
1299 |
| BBa_C0051
| Coding cI |
Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified
with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI
regulator (BBa_R0051). |
Vina S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
|
Escherichia coli |
775 |
| BBa_K1610300
| Coding YebF |
YebF is an E. coli motor protein. When other proteins are fused to the yebF sequence, yebF
can help secrete these proteins out of the E. coli membrane. |
Leon Yim, iGEM15_TAS_Taipei
|
Escherichia coli |
354 bp |
| BBa_E0040
| Coding GFP |
Green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt:
P42212 GFP (mut3b) |
jcbraff
|
Escherichia coli |
720 |
| BBa_E1010
| Coding RFP |
Monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm |
Drew Endy
|
Escherichia coli |
705 |
| pSB1C3
| Plasmid backbone |
High copy BioBrick assembly plasmid
pSB1C3 is a high copy number plasmid (RFC [10]) carrying chloramphenicol resistance.
The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
pSB1C3 has terminators bracketing its MCS which are designed to prevent transcription from
*inside* the MCS from reading out into the vector. The efficiency of these terminators is
known to be < 100%. Ideally we would construct a future set of terminators for bracketing a
MCS that were 100% efficient in terminating both into and out of the MCS region. |
Austin Che
|
|
2070 |
| pSB1A3
| Plasmid backbone |
High copy BioBrick assembly plasmid.
pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance.
The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
pSB1A3 has terminators bracketing its MCS which are designed to prevent transcription from
*inside* the MCS from reading out into the vector. The efficiency of these terminators is
known to be < 100%. Ideally we would construct a future set of terminators for bracketing a
MCS that were 100% efficient in terminating both into and out of the MCS region. |
Reshma Shetty & Tom Knight
|
|
2155 |
| pSB1K3
| Plasmid backbone |
High copy BioBrick assembly plasmid
This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.
|
Austin Che
|
|
2204 |
