The parts have been created as pieces of the UMA_MALAGA 2022 gene collection to form an integrated and coordinated family. It is based on a metabolic control mediated by a glucose-sensing mechanism in the environment.
First of all, the parts that make up the plasmid pSBC1Cel1 consist of the genes that forms the CAZymes enzyme complex (exoglucanase, endoglucanase, and β-glucosidase) and the gene corresponding to the fluorescent protein GFP. The codes linked to the Part Registry are: BBa_K4368000, BBa_K4368001, BBa_K4368003, and BBa_K4368006, each in order. Each enzyme acts together to degrade each type of cellulose linkage into glucose, forming an integrated plasmid.
Finally, the second plasmid (pSBA1Sta1) is composed of the enzyme GlgC, regulated by a promoter cI, the inhibitor protein cI and the fluorescent protein RFP.
The codes linked to the Part Registry in the order mentioned are: BBa_K4368004, BBa_K4368005, BBa_K4368007.GlgC is an enzyme that is part of the starch synthesis pathway. Overexpression of this enzyme induces the production and accumulation of starch-like molecules in the bacteria. It also presents the inhibitory protein cI, from which the cI promoters are controlled, depending on the glucose levels in the medium. Finally, as a fluorescent marker we have selected the RFP protein.