An Electro genetic Toolkit |
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To control gene expression, we developed a suite of genetic, electronic and digital tools that allow for
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An Electro genetic Toolkit |
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To control gene expression, we developed a suite of genetic, electronic and digital tools that allow for
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Electro-Genetic signal processing |
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We have broken down the signal processing problematic to 3 parts on
individual plasmids.
Like shelves in a workshop storing resistors and capacitors of specific values, we hope that users of our toolkit will construct library of interchangeable parts, so that entirely new components can be built up by swapping each of the 3 plasmids in a single transformations
We have standardised the way each of these parts communicates trhough combinaisons of transcription
factors | promoters.
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The Output plasmid hold a Transcriptional Unit where an electrically induced promoter is placed upstream of CinR, a transcription factor activating pCin in the presence of XXXX
High throughput screening of electrically inducible promoters |
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We developed a set of hardware and software tool to screen entire library of promoters, searching for electrically inducible promoters. We used this suite of tool in conjunction with the E. coli Promoter collection. These experiments allowed us to propose a collection of Bio-electronic bricks. |
| Firstname | Lastname | |
|---|---|---|
| John | Doe | john@example.com |
| Mary | Moe | mary@example.com |
| July | Dooley | july@example.com |
Monitoring and control of gene expression |
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We adapted and characterised open source hardware from biochemistry to be used in the context of synthetic biology and to control out INPUT Systems. Wedevelopped new route to monitor gene Expression and signal transduction of our system through an electronic sensor of our OUTPUT System |
A simple pipeline to connect gene expression to the web |
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Because of it bio-electronic nature, our toolkit represent a first corner stone to the building of an Internet of Living Things. As a simple proof of concept that our project enable seamless integration with existing digital technology, we rapidly (in less than 1 hour) built a pipeline (integrated with our our costum software and hardware) allowing to directly tweet bacterial population to control gene expression. Because of the electrical nature of our OUTPUT System, similar integration will be possible to enable bacterial populations to tweet back. We used this suite of tool in conjunction with the E. coli Promoter collection. These experiments allowed us to propose a collection of Bio-electronic bricks. |
