Parts

This page contains information about the parts created by the team.

Parts BBa_K3816009, BBa_K3816015, BBa_K3816005, BBa_K3816014, BBa_K3816008, BBa_K3816011, BBa_K3816007, BBa_K3816003 and BBa_K3816001 for Piri6, Masp2_2, Piri2, Masp2_1, Piri5, Masp1_102, Piri4, Tusp, Masp1_1 respectively from Team HK_CPU-WFN-WYY were used this year in the early cloning stage.

NTA-2REP-CT-SpyTag


Silk gene NT2RepCT-SpyTag, which is high soluble, pH sensitive and has high yield in E. coli expression system. The silk gene is combined with SpyTag and make it can be functionalized with various SpyCatcher-fused protein which can be used to bind with GFP and to epitope or purification tags. And also, two different restriction sites are added on both ends to allow ligation with different common plasmids.