Notebook

Documents of the dates we worked on our project.

June

Literature review
programmatically finding target sequences from NCBI
manually screening sequences, creating gene constructs and primer pairs (for screening and PCR) in Benchling
order reagents

August

1st-7th

Cloning of DNA 1-9 with SacI and SalI
Colony PCR screening

8th-14th

Cloning of DNA 1-9 into pET-SUMO
Colony PCR screening

15th-21th

Cloning of DNA 1-9 into pET-SUMO
Colony PCR screening
Mini-prep and sequencing for DNA 6

22nd-28th

Cloning of DNA 1-9 into pET-SUMO and pBAD-HisB
Colony PCR screening
Expression and SDS-PAGE of DNA 6

29th-4thSept

Cloning of DNA 1-9 into pET-SUMO and pBAD-HisB
Colony PCR screening

September

5th-11th

Cloning of DNA 1-9 into pET-SUMO and pBAD-HisB
Colony PCR screening
Mini-prep for DNA 2 and 9

12th-18th

UST Lab visit

19th-25th

Cloning of DNA 1-10 into pET-SUMO, pBAD-HisB and pET-22b
Colony PCR screening
Mini-prep for DNA 10
Expression and SDS-PAGE of DNA 6

26th-2ndOct

Expression and SDS-PAGE of DNA 6 and 10
Building a silk collection model

October

3rd-9th

Expression and SDS-PAGE of DNA 6 and 10
Purification of DNA 10
Protein concentrating
silk fabrication