Partnerships

    In addition to weekly reporting and communication with the FAFU-China team, we also pay attention to long-term and in-depth communication and cooperation with the FAFU team and other outstanding iGEM teams, as well as continuous improvement of the experiment through high-level academic meetings and field visits to finished product processing plants.
Overview
   Our team members came from different majors in school; here many schools gather to . Although each of us was only good at one area, we built a more perfect team through communication. With the communication of all people, we pooled the wisdom of all people to complete our project together and enhance our friendship. As the famous quote says：the collective is the source of power, all is the cradle of wisdom.
Our mutual mission
   Promote public science popularization.
   Exchange and explore the experimental methods.
   Integrate theories in different fields of biology.

1. Exchange and reflection with XMU-China

   During this phase, we maintained a high frequency of communication, and we visited a number of teams, companies and schools on a weekly basis. In addition to learning from their experience, we also provide food companies with our experimental ideas for beverage preparation.
    What have we acquired from it?
   We had an in-depth conversation with the igem team from Xiamen University and the FAFU team about the igem competition. In this exchange, we know how to carry out HP activities, and our HP activities should be based on the practical, and can be applied to the practical. For example, if we want to solve people's insomnia problem, we have to investigate the actual situation of insomnia people and find the solution to the problem according to their reasons.
Fig. 1.1 The picture is a group photo of three teams (1) Meeting process
14:00-14:30 Team check in & Opening ceremony
14:30-15:30 Presentations and Ideas Sharing of each team
15:30-16:00 Free Discussion
(2) Content of the meeting
      After the project presentation of each team, the remaining participants raised questions on the experimental design, modeling development, comprehensive practice and other aspects of the project, and shared their experiences.
      In the free discussion session in the afternoon, all the teams shared the four modules of experimental design, modeling development, hp development and team management in more detail. During the whole process, we put the intractable problems encountered in our team on the table to discuss and learn from each other's strengths. Thanks to these two exchanges, the team of our school gained some instructive questions.
Fig. 1.2 Three iGEM team members are having a heated discussion (3) Stage self-reflection
① Team mode
   Most teams are in danger of not completing the final experiment, so speeding up the progress of the experiment is the top priority.
   The experimental group avoid all people do the same thing, the best multi-threaded task
   All other activities revolve around the experimental group, focusing on fit.
   Capable students in the experimental group should gradually get out of the experiment, communicate with other groups, and control the whole situation.
② Experimental part
   Xiamen University introduced their research results in the past three years. It can be seen that innovation accounts for a large proportion of their projects and a mature narrative logic has been formed. For example, the newly introduced project has three parts: disease detection, treatment and prevention. The structure is very clear. We studied and modified the experimental design to improve the structure: the transfer of GABA key genes, the culture of high-yielding GABA longan, the extraction of GABA and so on.
③ Human Practice & Education
   The importance of communication between HP group and experimental group
   HP and the project content should be strongly related. We know how HP guides the focus of the experiment and the social responsibility of the project through research. And the progress of the experimental group has a strong guiding effect on HP.
   We consulted the data, collected how HP content and the experimental group interact with each other, and made the following plan:
HP influence experiment:
· Social needs influence experimental projects
· Laws, regulations and social policies affect experimental method raw materials, etc
· Market research to improve the experimental process
· Historical analysis, traditional customs, ethics and morals
· Promote experiments to produce more intuitive results and models
Effects of the experiment on HP:
· Decide to investigate relevant groups: direct users & potential product users
· Investigate relevant organizations & corporate groups: gains and losses
· Find relevant field experts
· Interdisciplinary groups such as computer, philosophy, ethics, legal experts for design software and other related supporting industries
Solve the problem of defective logic in HP group
   To simplify our thinking, and to get to the point, given the final formulation of our story, we'll match our own activities to the experiment. For example, in the experimental group, if the three parts of the design experiment are: the transfer of GABA key genes, the culture of high-yielding GABA longan and the extraction of GABA. Human practice is also divided into three parts: gene editing of the public and the questionnaire survey of sleep problems, the visit to the Longan base in the countryside, and the design of products (commercialization).

2. Joint practice——With China-FAFU

   "Together with the Taijiang Committee of the Communist Youth League and the Youth Activity Center of Taijiang District, we explored the mystery of our project with primary school students and another iGEM team China-FAFU.
Fig. 2.1-2.2 The members of the two teams are doing science popularization together    To achieve our common goal, the China-FAFU team and us organized a parent-child event in Taijiang District to educate teenagers and their parents about synthetic biology and gene editing. The activity was successful and the two teams got to know each other, paving the way for further cooperation.
   After that, the two teams held similar science popularization activities together many times. We held many planning meetings together and formed a deep cooperative relationship.
Fig. 2.3 The members from two teams are discussing about the plans of an activity

3. Preliminary exploration -- participating in high-end biomedical forum

   On June 11th, Fuzhou Strait International Convention and Exhibition Center was inaugurated in and FAFU-China participated in the "Fuzhou Ocean Research Institute 2022 Marine Biomedicine High-end Forum" sponsored by Fuzhou Ocean Research Institute, Fuzhou Ocean and Fishery Bureau, undertaken by Fuzhou Ocean Research Institute Marine Medicine RESEARCH and Development Center.
Fig.3.1&3.2 Attended the high-end Forum of Marine biomedicine    In the forum, FAFU-China is about communicate with experts on the gene editing technology of marine organisms, and further promote the development of marine biomedicine to unlock the mystery of gene editing.
   On the forum, experts and professors made relevant explanations on gene editing technology. Sun Song, academician of the International Eurasian Academy of Sciences and researcher of The Institute of Oceanology, Chinese Academy of Sciences gave a report entitled Deep-sea Extreme Environment and Life Detection: Special Organisms in Special Environments. Professor Xiaokun Zhang, dean of School of Pharmacy, Xiamen University, delivered a report entitled "Nuclear Receptor and Marine Innovative Drug Development". Professor Sulan Luo, academic dean of Guangxi University Medical College, delivered a report entitled "preclinical study of a new non-addictive analgesic snail toxin GeXIVA". Prof. Du Guanhua, academician of The International Eurasian Academy of Sciences and Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, delivered a report titled Progress in Pharmacology of Marine Drugs online. Professor Zhu Yizhen, the first dean from the school of Science and Technology of Pharmacy of Macau University, delivered a report entitled Discovery of Natural Drugs and Ideas of Marine Drug Development. In summary of the above reports, FAFU-China had a deeper understanding and reflection on the establishment of Longan GABA cell line and GABA gene editing. After the conference, we discussed the safety issues of gene editing technology and the development of biotechnology with experts and professors attending the conference.
   Gene editing is a major technological revolution, which undoubtedly brings many benefits to all aspects of human life. Especially taking the BBS on GABA editor for editing adopts the principle of critical inheritance of genes, dialectical view of the technology, then it is to establish the high GABA Longan cell line. The follow-up plans to use high yield cell fermentation tank production ramming foundation in the future is expected to realize factory production and extract the GABA, in order to better serve the pharmaceutical industry as well as benefit the cause of mankind.

4. Scientific Dialogue -- Attend the 2022 National Academic Conference

   This year, FAFU-CHINA sent representatives to attend the Chinese Society of Plant Physiology and Plant Molecular Biology. The annual meeting includes education and popular science forum, interdisciplinary forum, young scholars forum and so on. In addition to absorbing cutting-edge discoveries and technical means related to plant molecular biology, representatives of FAFU-China also communicated with scholars after the meeting about numerous problems in the experimental process of this project, and gained a great deal of knowledge.
Fig. 4.1 The picture shows representatives of FAFU-China attending the meeting Fig. 4.2 The picture shows the hall of Chinese Society of Plant Physiology and Plant Molecular Biology

5. Product improvement - Food processing plant survey

   On the afternoon of July 22nd, we went to Fuzhou Xicheng Food Co., LTD with Teacher Cao to conduct an investigation on Longan beverage processing.
   First, we had a meeting with the head of the West City Food Company about food production.
Fig. 5.1 The photo taken in front of Fuzhou Xicheng Food Co., LTD Factory.    The person in charge explained some basic knowledge of juice making to us, for example, the proportion of juice pulp made by the factory is usually 10%-20%. Apart from this, she showed us various types of beverage packaging shells, including PET bottles, PEP bottles, glass bottles and Tetra Pak paper containers. After the introduction of the basic knowledge from the manager, we chose Tetra Pak paper containers as the main reason is that Tetra Pak package has low cost, light weight and green environmental protection, and its shelf life is up to one year at room temperature. At the same time, aseptic cold filling technology can maximize the retention of nutrients in the beverage and effectively cover the pulp sedimentation phenomenon. After the consultation with Dr. Cao, we have reached an agreement with the factory that we plan to mass-produce Longan drinks. Before the mass production, we are responsible for determining the nutritional composition of the drinks in the pilot trial, and use the factory's trademark for processing and production. The packaging can be designed by us and the manufacturer after comparison.
Fig. 5.2 The picture shows the team members are watching the food processing process    After that, the manager led us to visit the factory assembly line, where we watched the complete process of Tetra Pak beverage processing and packaging. Also, we visited to learn the flow chart of the assembly line machine so as to have a basic understanding of the process of beverage production.
Fig. 5.3 The picture shows the team members are visit the processing workshop.    Finally, our team members took a group photo with the person in charge.