Preface:

  Due to the impact of COVID-19, the university has developed a series of anti-epidemic measures, and our team has faced a great challenge to build and develop. Our experiments were greatly affected, which also encountered many difficulties, lack of experimental materials and experimental reagents, but our FAFU-china team still did our best to present our project to the best.

Wet Lab

Molecular cloning
Members: Wenhui Ren, Qiqi Zhao, Liwen Luo, Honglin Yao, Huifei Zhu
Student leader: Qiqi Zhao
Mentor: Yuling Lin

CRISPR-Cas9 gene editing
Members: Wenhui Ren, Qiqi Zhao, Honglin Yao, Liwen Luo, Huifei Zhu
Mentor: Yuling Lin
Advisors: Zhuoyun Li, Chunyu Zhang, Chunwang Lai

Determination of GABA content in longan healing tissue
Members: Liwen Luo, Honglin Yao, Huifei Zhu
Student leader: Liwen Luo
Advisor: Chunwang Lai

Reagent procurement
Members: Wenhui Ren, Qiqi Zhao, Chen Yan, Xue Ying Zhang
Mentor: Yuling Lin

Plotting of GABA content curve
Members: Wenhui Ren
Advisor: Chunwang Lai

Connection and Transformation
Members: Wenhui Ren, Qiqi Zhao, Honglin Yao, Liwen Luo, Huifei Zhu
Student Leader: Wenhui Ren
Mentor: Yuling Lin

Quantitative Real-time PCR
Members: Wenhui Ren, Liwen Luo, Qiqi Zhao, Honglin Yao, Huifei Zhu
Advisor: Xueying Zhang

Data Analysis
Members: Wenhui Ren, Honglin Yao, Qiqi Zhao, Huifei Zhu, Liwen Luo
Advisor: Chunwang Lai
Hardware Design
Members: Wenhui Ren, Qiqi Zhao, Honglin Yao, Liwen Luo, Huifei Zhu

Model Design

Members:Jieru Liao, Jia Ying He, Lin Chen, Jing Jing Zhou
Jieru Liao is responsible for modeling the Genome-wide association study (GWAS), Enzymatic reaction and Michaelis-Menten equation. Jiaying He, Jingjing Zhou and Lin Chen are responsible for GABA production prediction, Evolutionary Computation Algorithm and Optimal location model.

Human Practice:

Cooperating Team: BNUZH-china, JLU-china, BIT-china, BUCT-china, NJU-china, NPU-china, NJMU-china, XMU-china
Members: Jiarui Chang, Yiming Jiang, Yiming Ma, Wenjun He, Nan Li
Student Leaders: Jia Rui Chang, Yiming Jiang
Mentor: Shijiang Cao

Social Survey
Members: Jia Rui Chang, Yiming Jiang, Yiming Ma, Wenjun He, Nan Li
Mentor: Shijiang Cao

Science Communication
Members: Jiarui Chang, Yiming Jiang, Yiming Ma, Wenjun He, Nan Li, Wenhui Ren, Qiqi Zhao, Honglin Yao, Liwen Luo Advisor: Chunwang Lai
Mentors: Shijiang Cao, Yuling Lin

Longan Beverage Production
Principal Designer: Yiming Jiang
Leaders: Yiming Jiang, Wenjun He
Lead person: Yiming Jiang, Wenjun He, Liwen Luo, Yongchao Pu, Huifei Zhu

Art Design:

Photography and Video Editing
Members: Ying Chen, Xiang, Yifan, Wang, Yongchao, Pu
Photography: Ying Chen, Xiang, Yifan, Wang
Editing: Yifan Wang, Ying Chen Xiang
Reviewer: Wenhui Ren, Jiarui Chang

Poster and graphic design
Producer: Feiyang Zhao, Yinglin Huang
Advisor: Ying Chen Xiang, Yifan Wang, Qiqi Zhao, Honglin Yao, Liwen Luo, Yiming Jiang, Yiming Ma, Wenjun He
Reviewer: Wenhui Ren, Jiarui Chang

WiKi:

Writing
Members: Wenhui Ren, Jiarui Chang, Liuping Guo, Yijia Zhuang
Mentors: Yuling Lin, Shijiang Cao
Reviewers: Wenhui Ren, Jiarui Chang
Advisors: Yingchen Xiang, Yifan Wang, Yiming Jiang, Qiqi Zhao, Honglin Yao, Liwen Luo, Yiming Ma, Wenjun He, Yongchao Pu, Huifei Zhu, Feiyang Zhao, Yinglin Huang, Nan Li

Wiki Production
Members: Liuping Guo, Yijia Zhuang
Tutor: Yuling Lin, Shijiang Cao
Art Design: Feiyang Zhao, Yinglin Huang
Reviewer: Wenhui Ren, Jiarui Chang
Advisors: Yingchen Xiang, Yifan Wang, Yiming Jiang, Qiqi Zhao, Honglin Yao, Liwen Luo, Yiming Ma, Wenjun He, Yongchao Pu, Huifei Zhu, Feiyang Zhao, Yinglin Huang

About synthetic biology:

  Our school does not offer a special course on synthetic biology, but our FAFU-china team still organizes team members to learn about synthetic biology systematically through various channels. In addition, we also organize our team members to study the contents of the manual on the iGEM official website so that they can better understand the rules and synthetic biology.

Acknowledgement:

·We would like to thank the leadership of the College of Horticulture, Fujian Agriculture and Forestry University for their great support to the FAFU-China team.
·Thanks for Yuling Lin and Shijiang Cao for providing us with access to the laboratory, reagents and instruments.
·We thank our brothers and sisters who have helped us in the laboratory.
·Thanks for The New Zealand Institute for Plant and Food Research Limited for providing the pGW3 plasmid.
·Thanks for Xueying Zhang for providing us with longan healing tissue at the beginning.
·We are grateful to TIANGEN, Thermo Fisher Scientific, Suzhou Kemin Biotechnology Co., Suzhou Grix Biotechnology Co., etc.