In the initial process of the experiment, we also focused on how to apply the technology to life, how to really help people. To achieve that, we have written a detailed and scientific business plan (you can click the file below to check), which mainly makes plans for the subsequent industrial processing of our team's technology.



      Our team presented BioLongan at the 7th CHINA International Internet + Competition. Here, we are able to develop a solid value proposition and sustainable business model, thanks to entrepreneurial consultants. After several meetings and modification of the simulated road show, we finally got a good result in the preliminary competition. Fig. 1. The picture shows the awards we won in the 7th International Internet + Innovation and Entrepreneurship Competition

Business Profile:

      The name of our business company, BioLongan is derived from "Synthetic Biology" and "Longan", aiming to make use of the gene editing technology of Synthetic Biology and rely on the strong academic resources and technical support of the Institute of Horticultural Plant Bioengineering and Fujian Fuqing Longan Science and Technology Academy. We have innovatively established high-yield GABA Longan cell line and created a new deep-processing industry chain where food and medicine come from the same source to fill the market gap.
      At the present stage (during the epidemic), the team reports the experimental progress weekly strictly in accordance with the experimental plan, and has basically completed the analysis of the bioassurance part, in order to gradually achieve the project effect.
      In the first stage, our team plans to complete the cultivation of high-GABA Longan as well as corporate sponsorship and financing. Furthermore, the preliminary project construction in the summer vacation of 2022 was formed. In the second stage, based on the existing GABA Longan products, our team actively cooperate with relevant domestic and foreign biological companies and agricultural product companies to expand the production of gene editing Longan. At this stage, GABA Longan industry will be actively introduced to rural areas, leading individual farmers to realize modern agriculture, and ultimately contributing to rural revitalization. The third stage will introduce deep processing resources such as medicine, food and medical treatment by 2025, improve the industrialization chain of deep processing Longan, fill the gap of GABA gene editing products in China, and seize market opportunities.
      BioLongan -- High-yield GABA Cell Factory focuses on Longan GABA gene editing innovation technology and makes its own contribution to accelerate social modernization.

Marketing strategy:

      Experts guide: currently, the purpose of our project is mainly to expand the domestic market, guide the teachers are all in the industry have high achievement in the field of experts and scholars, various resources and the flow of its own, help think-tank, experts in the field of the project is set for this project drainage, enlargement popularity in the related field.
      Media platform drainage: The team will popularize biotechnology knowledge, introduce and publicize related products through the official account on WeChat BiolonganFAFU. Additionally, we also open an online consultation channel on the basis of the official account. Later, the project also plans to create Zhihu and Douyin short video accounts for further promotion.
      Participate in biotechnology and food science or related competition: this project is mainly achieved by biotechnology content of GABA in Longan, thus producing a series of derivatives, such as, Longan drinks cool paste, therefore improve the project in the field of plant biotechnology and food science visibility for marketing have great help. The project plans to actively participate in some competitions in related fields, such as College Students Life Science Innovation and Entrepreneurship Competition, Food Safety Science Popularization Innovation Competition, etc.
      Exchange views and learning from relevant enterprises and universities: our project plans to exchange knowledge and experience with universities and enterprises in relevant fields, which will not only facilitate the diversion of the project, but also obtain information effectively to accelerate the growth of our project.

Initial product production

(1) The preparation process of longan beverage
Preliminary experiments:
         Objective:
      To confirm the amount of citric acid, sugar and essence additives needed to prepare Longan beverage.
        Experimental procedure: Pour the original slurry into a 50ml centrifuge tube, centrifuge it at 5000R for 15min and transfer the supernatant to the pot after centrifugation. Then, add 160ml drinking water to the pot and set the fire.

      When the solution in the pan is about to boil, turn down the heat and pour the solution in the beaker into the pan, stirring as you add. Then cook on a high heat for 2-3 minutes. Let it cool and taste it to see if the sweetness and acidity are right, to determine the amount of citric acid and granulated sugar.
      After determining the amount of citric acid and sugar, repeat the above operation. And then finally boil off the heat, add the right amount of essence, to be cooled after tasting, determine whether the fragrance is appropriate, so as to determine the amount of essence.
Results of the preliminary experiment:
      After repeated tasting, it is determined that the amount of citric acid is about 0.11%, the amount of sugar is about 1%, and the amount of essence is about 0.0002%.
Experimental procedure:
      Objective: To investigate people's taste preference by preparing four kinds of formula drinks with different pulp and citric acid content.
Experimental procedure:
1. Pour the original longan pulp into a 50ml centrifuge tube and place it in a centrifuge at 5000R for 15min. After centrifugation, take the supernatant into a 500ml cylinder.
2. We Set A/B/C/D four recipes, respectively add the following amount of materials to the pot and stir:

3.Add the following ingredients to a 100ml beaker, add a small amount of water and stir with a glass rod.

      After dissolving, pour the solution into the pot of each formula. When the solution in the pot is about to boil, turn down the heat, pour the solution in the beaker into the pot, stir while adding, and then cook on high fire for 2-3 minutes.
4. Turn off the fire after boiling, add a certain amount of essence to the pot, glass rod appropriate stirring, juice into 300ml PP bottle with a spoon, can to about 5mm from the mouth of the bottle, remove the air in the container with liquid, and then screw the lid to seal. After sealing, pour the bottle for 15min, and finally put it in cold water to cool.
5. Take out the PP bottle after cooling and mark the A/B/C/D formula. Fig. 3.1-3.3 The above three pictures are our successful processing and mass production of Longan drinks (2) Questionnaire analysis of longan beverage
      To investigate the how do people like our Longan beverage, we gave out questionnaires to improve the formula for the better taste.
      We have designed four formulations of flavored drinks. The survey found that in Fig. 3.4 Formula C (containing 25% Longan protoplasmic, 1% sugar and 0.12% citric acid) was the most popular, while Formula D, containing 20% Longan protoplasmic and 0.1% citric acid, was barely liked by a few. The original pulp and citric acid content of Formula B and Formula A beverages are between Formula D and Formula C. Questionnaires show that most people believe that drinks with acidic taste are better, so we have increased the content of pulp and citric acid in our beverage processing to meet the needs of more people's taste. Fig. 3.4 Result of the question “which formula do you think is more suitable for your taste?” Fig. 3.5 Result of the question “What taste do you like?”
      In the questionnaire, we also asked respondents for suggestions to improve the taste. People generally feel satisfied about our drinks and reckon they are superior to other drinks as the GABA (an amino acid extracted from longan) in our drinks has a sleep-aiding and anxiety-relieving effect. Hence, we will further improve the taste of our drinks and try to meet the market demand so that our products can be launched and promoted as soon as possible. Fig. 3.6 The preferences of people who have tasted the drink