Parts

BASIC PARTS

Part number

Part name

Description

Length

BBa_K4165062

Lac operator

APGS-21a Lac Operator

25 bp

BBa_K4165264

RBS 4

Coh/Doc

60 bp

BBa_K4165263

RBS 3

Trim

41 bp

BBa_K4165262

RBS 2

tau

61 bp

BBa_K4165062

RBS 1

Ubq C

43 bp

BBa_K4165075

RBS 5

Composite trim and UBE2v2

42 bp

BBa_K4165260

E2 RBS

Ribosomal binding site used for the expression of the UBE2N - UBE2VE - UBE2W

24 bp

BBa_K4165001

Trim21 (Truncated)

Truncated tripartite motif-containing 21 (TRIM21)

804 bp

BBa_K4165003

Coh2

Cohesin type 2 enzyme binds to its counterpart DocS (BBa_K3396000)

414 bp

BBa_K4165006

TD28REV

Tau binding peptide

39 bp

BBa_K4165007

WWW

Tau binding peptide

33 bp

BBa_K4165009

Tau

Human tau protein isoform 0N4R.

1152 bp

BBa_K4165011

UBE2N

Ubiquitin-conjugating E2 ligase

459 bp

BBa_K4165015

UBE2W

Ubiquitin-conjugating E2 ligase

489 bp

BBa_K4165013

UBE2V2

Ubiquitin-conjugating E2 ligase

438 bp

BBa_K4165014

UBC

ubiquitin C which is essential in the degradation of misfolded proteinse

231 bp

BBa_K4165020

6-His

6x Histidine tag used for purification and characterization of proteins

21 bp

BBa_K4165070

GST Tag

Glutathione S-Transferase tag

654 bp

BBa_K4165068

(G4S)3 Linker

A flexible 5 aa Glycine-Serine linker

15 bp

BBa_K4165204

PHF

Paired Helical Filament of tau protein

18 bp

BBa_K4165205

PHF*

PHF* of tau protein

18 bp

COMPOSITE PARTS

Part number

Part name

Description

Length

BBa_K4165253

GST - Docs

Dockerin S protein tagged with Glutathione S-transferase

864 bp

BBa_K4165254

GST - Coh2

Cohesin 2 protein tagged with GST

1068 bp

BBa_K4165255

GST-Coh2-G4S- TD28REV

Coh2 linked to tau binding peptide TD28rev

1444 bp

BBa_K4165256

GST - Coh2 - L - WWW

Coh2 linked to tau binding peptide WWW

1438 bp

BBa_K4165200

His-Trim21-(GGGGS)3-Coh2

trim21 E3 ligase linked to coh2

1282 bp

BBa_K4165201

His-Trim21-GSGS-Coh2

trim21 E3 ligase linked to coh2

1249 bp

BBa_K4165202

His-Trim21-(GGGGS)3-Doc2

trim21 E3 ligase linked to Docs

1078 bp

BBa_K4165203

His - Trim21 - GSGS - Docs

trim21 E3 ligase linked to Docs

1045 bp

BBa_K4165177

T7 Promoter - RBS - His - TRIM21 - T7 Terminator

Composite part to express the His_trim21

955 bp

BBa_K4165074

T7 Promoter - RBS - His - TRIM21- Linker - Docs - T7 Terminator

Composite part to express the His_trim21 kinked to Docs

1214 bp

BBa_K4165072

T7 Promoter - RBS- GST - Docs - T7 Terminator

Composite part to express GST-Docs

1016 bp

BBa_K4165167

T7 Promoter - RBS - His-Docs - T7 Terminator

Composite part to express His-Docs

383 bp

BBa_K4165171

T7 Promoter - RBS- GST - CoH2 - T7 Terminator

Composite part to express GSt-Coh2

1220 bp

BBa_K4165169

T7 Promoter - RBS- His - CoH2 - T7 Terminator

Composite part to express His-Coh2

587 bp

BBa_K4165232

T7 Promoter - RBS- GST - Coh2 - L - TD28REV - T7 Terminato

Composite part to express GST-coh2 linked to tau binding peptide TD28rev

1274 bp

BBa_K4165173

T7 Promoter - RBS- GST - Coh2 - L - WWW - T7 Terminator

Composite part to express GST-coh2 linked to tau binding peptide WWW

1298 bp

BBa_K4165175

T7 Promoter - RBS - His - Tau - T7 Terminator

Composite part to express His-Tau

1326 bp

BBa_K4165233

T7 Promoter - RBS - His - UBE2N - T7 Terminator

Composite part to express His-UBE2N

614 bp

BBa_K4165235

T7 Promoter - RBS - His - UBE2W - T7 Terminator

Composite part to express His - UBE2W

644 bp

BBa_K4165238

T7 Promoter - RBS - His - UBE2V2 - T7 Terminator

Composite part to express His - UBE2V2

572 bp

BBa_K4165240

T7 Promoter - RBS - His - UBC - T7 Terminator

Composite part to express His - UBC

387 bp

PART COLLECTION

Part number

Part name

Length

BBa_K4165206

Coh2 _(M4W39) with G4S linker

459 bp

BBa_K4165208

CoH2_(M4W39) with (GS)2 linker

472 bp

BBa_K4165209

COH2_WINK_G4S linker

529 bp

BBa_K4165210

COH2_WINK_(GS)2 linker

472 bp

BBa_K4165211

COH2_MMD3REV_G4S_linker

527 bp

BBa_K4165212

COH2_MMD3REV_GSGS_linker

478 bp

BBa_K4165213

COH2_MINK_G4S

521 bp

BBa_K4165214

COH2_MINK_GSGS

472 bp

BBa_K4165215

COH2_TD28_G4S

524 bp

BBa_K4165216

COH2_TD28_GSGS

475 bp

BBa_K4165217

COH_WWW_(G4S)3

522 bp

BBa_K4165218

COH_WWW_GSGS

473 bp

BBa_K4165219

COH2_TD28rev_G4S

528 bp

BBa_K4165220

COH2_TD28rev_GSGS

479 bp

BBa_K4165221

COH2_MMDP6_G4S

521 bp

BBa_K4165222

COH2-MMDP6-GSGS

472 bp

Plug Sink system

BASIC PARTS

Part number

Part name

Description

Length

BBa_K4165012

Seed

Amyloid 𝛽 seed (37-42)

21 bp

BBa_K4165000

H1A

synthetic peptide that binds to the PDZ domain of HTRA1

27 bp

BBa_K4165008

Inhibitor 1

WAP-four disulfide core domain 13 serine protease inhibitor.

279 bp

BBa_K4165016

PGS21aRBS

plasmid for Plug Sink system

44 bp

BBa_K4165095

RBS 6

22 bp

BBa_K4165010

Inhibitor 2

Human serine protease inhibitor Kazal type 8 (SPINK8)

291 bp

BBa_K4165005

Amyloid Beta

Amyloid β Protein Fragment 1-42 (Aβ 1-42)

132 bp

BBa_K4165004

HtrA1

Truncated Serine Protease HtrA1

975 bp

BBa_K4165095

AB-RBS

RBS for amyloid beta expression.

22 bp

Composite PARTS

Part number

Part name

Length

BBa_K4165257

GST - Seed

675 bp

BBa_K4165258

GST - TD28REV

693 bp

BBa_K4165259

GST - WWW

687 bp

BBa_K4165178

Biobrick - seed

811 bp

BBa_K4165174

Biobrick - H1A

817 bp

BBa_K4165172

Biobrick - inhibitor 1

436 bp

BBa_K4165170

Biobrick - inhibitor 2

448 bp

BBa_K4165179

Biobrick - TD28REV

829 bp

BBa_K4165176

Biobrick - WWW

823 bp

BBa_K4165168

Biobrick - Amyloid Beta

289 bp

BBa_K4165149

Biobrick - His-HTRA1

1132 bp

PART COLLECTION

Linkers

Tau Binding Peptides

Switches

Amyloid Beta Peptides

HtrA1 Inhibitors

HtrA1 Binding Peptides

Clamps

We added several linkers to our registry that can be used in our Plug Sink system.

Part number

part

Type

BBa_K4165017

GGGGSG

Basic

BBa_K4165018

GGSGGGG

Basic

BBa_K4165019

GGSGGGGG

Basic

BBa_K4165067

GGGG

Basic

BBa_K4165068

G4S

Basic

BBa_K4165065

GSGS

Basic

BBa_K4165066

GSGSG

Basic

BBa_K4165063

GSGSGSG

Basic

BBa_K4165064

GGGGSGG

Basic

BBa_K4165224

GGSGGG

Basic

BBa_K4165226

GGSGG

Basic

BBa_K4165236

GGSGGGGSGG

Basic

Different tau binding peptides that can be used with our system and some of these peptides were tested with other proteins.

Part Number

Part

Type

BBa_K4165139

TLKIVW

Basic

BBa_K4165141

MMD3

Basic

BBa_K4165142

MMDREV3

Basic

BBa_K4165143

KNT

Basic

BBa_K4165144

MINK

Basic

BBa_K4165145

TD28

Basic

BBa_K4165146

WINK

Basic

BBa_K4165148

W-MINK

Basic

BBa_K4165150

APT

Basic

BBa_K4165151

M4W39

Basic

BBa_K4165152

ISAD1

Basic

BBa_K4165153

WMW

Basic

BBa_K4165154

MM3

Basic

BBa_K4165156

MM2

Basic

BBa_K4165157

MMDP6

Basic

BBa_K4165158

P-NH

Basic

BBa_K4165159

Basic

BBa_K4165160

R32

Basic

Composite parts consists of the inhibitor linked to targeted protein binding peptides and the protease binding peptide. All combined to inhibit the protease activity till it reaches the target protein and be active to degrade. We used this switch with our plug-sink system.

Part Number

Part

Type

BBa_K4165021

HtrA1 switch 1

Composite

BBa_K4165022

HtrA1 switch 2

Composite

BBa_K4165023

HtrA1 switch 3

Composite

BBa_K4165024

HtrA1 switch 4

Composite

BBa_K4165025

HtrA1 switch 5

Composite

BBa_K4165026

HtrA1 switch 6

Composite

BBa_K4165027

HtrA1 switch 7

Composite

BBa_K4165028

HtrA1 switch 8

Composite

BBa_K4165029

HtrA1 switch 9

Composite

BBa_K4165030

HtrA1 switch 10

Composite

BBa_K4165031

HtrA1 switch 11

Composite

BBa_K4165032

HtrA1 switch 12

Composite

BBa_K4165033

HtrA1 switch 13

Composite

BBa_K4165034

HtrA1 switch 14

Composite

BBa_K4165035

HtrA1 switch 15

Composite

BBa_K4165036

HtrA1 switch 16

Composite

BBa_K4165037

HtrA1 switch 17

Composite

BBa_K4165038

HtrA1 switch 18

Composite

BBa_K4165039

HtrA1 switch 19

Composite

BBa_K4165040

HtrA1 switch 20

Composite

BBa_K4165041

HtrA1 switch 21

Composite

BBa_K4165042

HtrA1 switch 22

Composite

BBa_K4165043

HtrA1 switch 23

Composite

BBa_K4165044

HtrA1 switch 24

Composite

BBa_K4165045

HtrA1 switch 25

Composite

BBa_K4165046

HtrA1 switch 26

Composite

BBa_K4165047

HtrA1 switch 27

Composite

BBa_K4165048

HtrA1 switch 28

Composite

BBa_K4165049

HtrA1 switch 29

Composite

BBa_K4165050

HtrA1 switch 30

Composite

BBa_K4165051

HtrA1 switch 31

Composite

BBa_K4165052

HtrA1 switch 32

Composite

BBa_K4165053

HtrA1 switch 33

Composite

BBa_K4165054

HtrA1 switch 34

Composite

BBa_K4165055

HtrA1 switch 35

Composite

BBa_K4165056

HtrA1 switch 36

Composite

BBa_K4165057

HtrA1 switch 37

Composite

BBa_K4165058

HtrA1 switch 38

Composite

BBa_K4165059

HtrA1 switch 39

Composite

BBa_K4165060

HtrA1 switch 40

Composite

Different ِAmyloid Beta binding peptides that can be used with our system and some of these peptides were tested with other proteins.

Part Number

Part

Type

BBa_K4165180

Amyloid beta peptide

Basic

BBa_K4165181

Amyloid beta peptide 1

Basic

BBa_K4165182

Amyloid beta peptide 2

Basic

BBa_K4165183

Amyloid beta peptide 3

Basic

BBa_K4165184

Amyloid beta peptide 4

Basic

BBa_K4165185

Amyloid beta peptide 5

Basic

BBa_K4165186

Amyloid beta peptide 6

Basic

BBa_K4165187

Amyloid beta peptide 7

Basic

BBa_K4165188

Amyloid beta peptide 8

Basic

BBa_K4165189

Amyloid beta peptide 9

Basic

BBa_K4165190

Amyloid beta peptide 10

Basic

BBa_K4165191

Amyloid beta peptide 11

Basic

BBa_K4165192

Amyloid beta peptide 12

Basic

BBa_K4165193

Amyloid beta peptide 13

Basic

BBa_K4165194

Amyloid beta peptide 14

Basic

BBa_K4165195

Amyloid beta peptide 15

Basic

BBa_K4165196

Amyloid beta peptide 16

Basic

BBa_K4165197

Amyloid beta peptide 17

Basic

BBa_K4165198

Amyloid beta peptide 18

Basic

Inhibitors used in our Plug Sink system to inhibit the activity of Htra1 when the target protein doesn’t exist.

Part Number

Part

Type

BBa_K4165076

SPINK1

Basic

BBa_K4165077

WAP-four disulfide core domain 10A serine protease inhibitor.

Basic

BBa_K4165078

SPINK6

Basic

BBa_K4165079

SPINK2

Basic

BBa_K4165080

SPINK7

Basic

BBa_K4165081

SPINK 4

Basic

BBa_K4165082

SPINK 9

Basic

BBa_K4165083

SPINT 3

Basic

BBa_K4165084

SPINK 13

Basic

BBa_K4165085

SPINK 14

Basic

BBa_K4165086

SPINT 4

Basic

BBa_K4165087

WAP-four disulfide core domain 12 serine protease inhibitor.

Basic

BBa_K4165088

WAP-four disulfide core domain 14 serine protease inhibitor.

Basic

BBa_K4165089

WAP-four disulfide core domain 2 serine protease inhibitor.

Basic

BBa_K4165090

WAP-four disulfide core domain 6 serine protease inhibitor.

Basic

BBa_K4165091

SLPI (Secretory leukocyte peptidase inhibitor).

Basic

BBa_K4165092

EPPIN (epididymal peptidase inhibitor).

Basic

BBa_K4165093

Histocompatibility minor serpin domain (HMSD).

Basic

BBa_K4165094

Phosphatidylethanolamine-binding protein 1(PEBP1).

Basic

Synthetic peptides designed to bind to the PDZ domain of HtrA1.

Part Number

Part

Type

BBa_K4165096

HTRA Binding Peptide 2

Basic

BBa_K4165097

HTRA Binding Peptide 3

Basic

BBa_K4165098

HTRA Binding Peptide 4

Basic

BBa_K4165140

HTRA Binding Peptide 5

Basic

BBa_K4165100

HTRA Binding Peptide 6

Basic

BBa_K4165101

HTRA Binding Peptide 7

Basic

BBa_K4165102

HTRA Binding Peptide 8

Basic

BBa_K4165103

HTRA Binding Peptide 9

Basic

BBa_K4165104

HTRA Binding Peptide 10

Basic

BBa_K4165105

HTRA Binding Peptide 11

Basic

BBa_K4165106

HTRA Binding Peptide 12

Basic

BBa_K4165107

HTRA Binding Peptide 13

Basic

BBa_K4165108

HTRA Binding Peptide 14

Basic

BBa_K4165109

HTRA Binding Peptide 15

Basic

BBa_K4165110

HTRA Binding Peptide 16

Basic

BBa_K4165111

HTRA Binding Peptide 17

Basic

BBa_K4165116

HTRA Binding Peptide 22

Basic

BBa_K4165117

HTRA Binding Peptide 23

Basic

BBa_K4165118

HTRA Binding Peptide 24

Basic

BBa_K4165119

HTRA Binding Peptide 25

Basic

BBa_K4165120

HTRA Binding Peptide 26

Basic

BBa_K4165121

HTRA Binding Peptide 27

Basic

BBa_K4165122

HTRA Binding Peptide 28

Basic

BBa_K4165123

HTRA Binding Peptide 29

Basic

BBa_K4165128

HTRA Binding Peptide 34

Basic

BBa_K4165129

HTRA Binding Peptide 35

Basic

BBa_K4165130

HTRA Binding Peptide 36

Basic

BBa_K4165131

HTRA Binding Peptide 37

Basic

BBa_K4165132

HTRA Binding Peptide 38

Basic

BBa_K4165133

HTRA Binding Peptide 39

Basic

BBa_K4165124

HTRA Binding Peptide 30

Basic

BBa_K4165125

HTRA Binding Peptide 31

Basic

BBa_K4165126

HTRA Binding Peptide 32

Basic

BBa_K4165127

HTRA Binding Peptide 33

Basic

BBa_K4165134

HTRA Binding Peptide 40

Basic

BBa_K4165135

HTRA Binding Peptide 41

Basic

BBa_K4165136

HTRA Binding Peptide 42

Basic

BBa_K4165137

HTRA Binding Peptide 43

Basic

BBa_K4165138

HTRA Binding Peptide 44

Basic

BBa_K4165112

HTRA Binding Peptide 18

Basic

BBa_K4165113

HTRA Binding Peptide 19

Basic

BBa_K4165114

HTRA Binding Peptide 20

Basic

BBa_K4165115

HTRA Binding Peptide 21

Basic

BBa_K4165000

H1A

Basic

Part of the Plug Sink system composed of two bending peptides linked together responsible for targeting of proteins.

Part Number

Part

Type

BBa_K4165241

Clamp 1

Composite

BBa_K4165242

Clamp 2

Composite

BBa_K4165243

Clamp 3

Composite

BBa_K4165244

Clamp 4

Composite

BBa_K4165246

Clamp 5

Composite

BBa_K4165248

Clamp 6

Composite

BBa_K4165249

Clamp 7

Composite

BBa_K4165250

Clamp 8

Composite

BBa_K4165223

Clamp 9

Composite

BBa_K4165225

Clamp 10

Composite

BBa_K4165227

Clamp 11

Composite

BBa_K4165228

Clamp 12

Composite

BBa_K4165229

Clamp 13

Composite

BBa_K4165231

Clamp 14

Composite

BBa_K4165230

Clamp 15

Composite

BBa_K4165234

Clamp 16

Composite

BBa_K4165237

Clamp 17

Composite

BBa_K4165239

Clamp 18

Composite

BBa_K4165251

Clamp 19

Composite

Snitch system

Plug Sink system

Plug Sink system

Snitch