During our project, each member participated in the literature search and attended brainstorming sessions to decide on the right scientific idea that contributes to the welfare of both our community and worldwide. Moreover, all of us have taken tours to seek funds from international and national companies. Afterward, we divided the work into three parts: dry lab, wet lab, and creative team.
PIs
Professor of Analytical Chemistry,
our PI, helped us in every step.
Starting from the registration in iGEM and introducing us
to our faculty professors and convincing the authorities of our idea
Pushing our jamboree
registration till the last day.
Dean of our faculty and Professor of Bioinorganic Chemistry
Our secondary PI who supported
us during our journey and convinced the university of our idea.
Instructor
He helped through
the fundraising process
Covered all the paperwork and logistics, revised the proposal
and contract terms of our sponsors
Established the event and awareness campaigns, and
brainstormed design ideas
The one who started it all,
missing something? Just call Lina, and describe the one who helped us from the start in two sentences.
Lina was a team member of the CU team in 2019.
At the time of the pandemic, when a whole semester was
canceled from our syllabus, She stepped in and helped us learn the basics of molecular and synthetic
biology.
She pushed us to start at a very hard time worldwide and put us on the right way toward the
best adventure in our life. Not to mention her role afterward as our instructor.
She is certainly
our savior, always there to provide a missing reagent.
They call them supervisors;
she is our superwoman.
Menna was also a team member of the CU team in 2019, and she is a teaching
assistant in our faculty.
Crossing paths with her is the best thing that happened to us; we wouldn’t
have been where we are today without her.
We learn something from her every day in every aspect of
iGEM, starting from the brainstorming, Wet lab, and Dry lab to even preparing for the Jamboree closing
ceremony.
It is not just about giving you what you ask for; she always knows how to push you to grow,
learn and get to the thing you want.
Mentors
Our mentor and one of the iGEM ambassadors,
and a pioneer in the Engineering committee.
Wet Lab
The team that performed every step to prove our concept with experimental
design starting from part suspension to each part characterization.
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Wet lab team leader,
organizing the time plan for the wet-lab members, dividing the task equally between them )
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
(Team member)
- Protocols optimization
-Daily Buffer stocks preparation
-Cloning protocols (competent cells preparation, restriction, ligation, transformation)
-Protein characterization (Native protein extraction, SDS-PAGE, BCA and Bradford assay, Tau aggregation)
Dry Lab
The work was concerned with every simulation either by a mathematical model or through Molecular dynamic simulation for our drug-discovery process; it involved learning the following language to perform those tasks: R, Python, Bash scripting, and Matlab.
(Dry lab team leader, organizing the time plan for the dry-lab members,
dividing the task equally between them, worked on)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic parts assembly
-Scripts: HTRA1 enzymatic activity
(Team leader, organizing the time plan for all of the team members,
dividing the task equally between them, worked on)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: software pipeline collection between codes
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: software HTRA1 enzymatic activity code, Linux automation code for Gromacs, statistical analysis code.
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: software pipeline collection between codes
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: Transcription rate code, statistical analysis code
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: Translation rate, Ubiquitintion rate code, automation of light dock code
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: Transcription and Translation rate code, post-MD analysis code
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: Transcription rate code, statistical analysis code
(Team member)
- Mathematical Modelling equation building
-Drug discovery process: Modeling, QA, Docking, MD simulations, and post-MD analysis
-Genetic-parts assembly
-Scripts: Transcription rate code, Post-MD analysis code
Creative Team
This team has been concerned with every human practice, event planning, education and communication campaigns, meeting experts, collaborations, partnerships, and wiki page coding and establishment.
(Team member)
- Scripting and designing the
sketches, Zekra book on canvas
-Video Making and editing
-Event planning
-Human Practices, education events establishment
-Social media communication
(Creative team leader, organizing the time plan for the creative team members,
dividing the task equally between them, worked on)
- Video Making and editing
-Event planning
-Human Practices, education events establishment
-Social media communication
-Collaboration ideas
(Team member)
- Designing using photoshop
-Brainstorming ideas for wiki theme and setting color pallets
-Our main hero in the promotion video
(Team member)
- Event planning
-Human Practices, education events establishment, especially the idea of the miniGEM competition
-Social media communication
-Collaboration ideas
-Translation
-Wiki page coding and construction
(Team member)
-Event planning
-Human Practices, education events establishment
-Social media communication
-Collaboration ideas
-Subtitles
-Translation
-Wiki page coding and construction
(Team member)
- Designing using illustrator
-Brainstorming ideas for wiki theme and setting color palettes
(Team member)
-Voice over
(Team member)
-The book's and brochure's drawings.
-The events' frames and decorations
-Brainstorming for promotion video
Acknowledgment
Prof.Dr. at botany and microbiology department,
faculty of science cairo universty.
He was very helpful in attending meetings for us to know project progress and give us DMSO to
use in the preparation of competent cell
He was actively helping us with coding for the wiki pages.
He spent many days just like a real roster member giving the best presentation of our work
He is also expected to join iGEM in 2024.
Constantly gave us advice for coding the wiki.
A very helpful person and he helped us alot in wiki coding
She was an active team leader and wet lab member for one year and a half as well as she was from the reasons for team construction and joined in brainstorming for project idea.
He was an active creative team member for one year and a half and joined in brainstroming for project idea.
She was an active creative team member for one year and a half and joined in brainstroming for project idea.