Part Suspension
Restriction Reaction
T4 Ligation
Gel Electrophoresis
LB Preparation.
Glycerol stock and cell reviving
Bacterial Transformation
Manual Plasmid Miniprep
Plasmid Miniprep Promega Kit
Plasmid Miniprep Simply Kit
DNA Gel Extraction kit
Protein Extraction Chemical Lysis Method
Trim-21 Extraction
E2s Extraction
Coh and Doc Extraction
Ubiquitin C Extraction
UBE1 Extraction
Tau Extraction.
Bradford assay
BCA Assay
SDS PAGE
Affinity Chromotography
GST Purification
Tau Phosphorylation and aggregation
Thioflavin T Assay
Pull-down assay
In Vitro Ubiquitination