Project
Description
Contributions
Proof of Concept
Engineering
Results
Safety
Judging
Awards
Preservation
Overview
Nisin
Modelling
Golden Gate Assembly
Measurement
Protection
Overview
PHB
Modelling
Improved Part
Uniaxial Testing
Production
Overview
Fruit Waste Media
Co-culture
Co-culture Modelling
BioSculpting
Colouring
Compostability
Human Centered Design
Integrated Human Practices
Collaborations
Entrepreneurship
Implementation
Communication
Education
Partnership
Sustainability
Cost and Ease of Use
Branding
Software and Modelling
Modelling Overview
Software Overview
SAMARA
INN
Parts
Overview
Part Collection
Validation
Team
Team
Attributions
Notebook
Protocols
Appendix
Protocols
This page contains all protocols used in the lab
Basic Synthetic Biology Protocols
Agarose Gel Electrophoresis
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Calgary2022_Agarose_Gel_Electrophoresis.pdf
Autoinduction
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Calgary2022_Autoinduction.pdf
Bacterial Transformations
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Calgary2022_BacterialTransformations.pdf
DNA Excision from Low Melting Point Gels
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Calgary2022_DNA_Excision_Low_Melting_Point_Gels.pdf
Glycerol Stock
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Calgary2022_GlycerolStock.pdf
LB Agar Plates
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Calgary2022_LBAgarPlates.pdf
LB Liquid Broth
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Calgary2022_LBLiquidBroth.pdf
Ligation of Digested Bacteria
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Calgary2022_ligation-digested-dna.pdf
Plasmid Miniprep
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Calgary2022_plasmid-miniprep.pdf
Regular PCR
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Calgary2022_regpcr.pdf
Rehydration of Registry DNA
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Calgary2022_RehydrationRegistryDNA.pdf
Restriction Digest
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Calgary2022_restrictiondigest.pdf
SDS-PAGE
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Calgary2022_SDSPAGE.pdf
Sequencing
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Calgary2022_sequencing.pdf
Western Blot
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Calgary2022_western-blot.pdf
Serial Dilution for Spread Plates
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Calgary2022_SerialDilutionforSpreadPlates.pdf
Co-culture Protocols
Break Down BC With Cellulase
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Calgary2022_breakdownBCwithCellulaseProtocol.pdf
Co-culture
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Calgary2022_CoCultureProtocol.pdf
Colony Morphology For Cell Quantification
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Calgary2022_ColonyMorphologyForCellQuantificationProtocol.pdf
Comparing Purification Methods for Conditioned Media
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Calgary2022_ComparingPurificationMethodsforConditionedMedia.pdf
Determining RPM For Optimal BC Yield
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Calgary2022_DeterminingRPMForOptimalBCYieldProtocol.pdf
Fluorescence Microscopy With GFP-Enriched BC
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Calgary2022_FluorescenceMicroscopyWithGFPEnrichedBC.pdf
GFP Plasmid Insertion Into
E. coli
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Calgary2022_GFPPlasmidInsertionInto
E. coli
Protocol.pdf
HS Agar Plates
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Calgary2022_HSAgarPlatesProtocol.pdf
HS Media Standard Protocol
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Calgary2022_HSMediaStandardProtocol.pdf
Intermittent Feeding
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Calgary2022_IntermittentFeedingProtocol.pdf
K. xylinus
Plating Methods
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Calgary2022_
K. xylinus
PlatingMethodsProtocol.pdf
Quantification of Bacteria Using Plates
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Calgary2022_QuantificationofBacteriaUsingPlatesProtocol.pdf
Quantifying Wet and Dry BC Yield
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Calgary2022_QuantifyingWetandDryBCYieldProtocol.pdf
Rehydration of BC Using GFP-Enriched Solution
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Calgary2022_RehydrationofBCUsingGFPEnrichedSolutionProtocol.pdf
Standing Cultures Using Broth Culture
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Calgary2022_StandingCulturesUsingBrothCultureProtocol.pdf
STEP 1: Monoculture Protocol
K. xylinus
E. coli
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Calgary2022_STEP1MonocultureProtocolKXylinusEcoli.pdf
STEP 2: Switching Media Between
K. xylinus
and
E. coli
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Calgary2022_STEP2Switching MediaBetweenKXylinusandEcoli.pdf
Testing Efficacy of NaHCO3(aq) Wash
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Calgary2022_TestingEfficacyofNaHCO3(aq)Wash.pdf
PHB Protocols
3% Glucose Media
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Calgary2022_3perc.pdf
Bradford Protein Assay
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Calgary2022_BradfordProteinAssayProtocol.pdf
Flow Cytometry
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Calgary2022_FlowCytometry.pdf
Nile Red LB Agar Plates
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Calgary2022_NileRedLBAgarPlates.pdf
Phasin Western Blot
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Calgary2022_phasinwesternblot.pdf
PHB Extraction
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Calgary2022_phbextractionprotocol
Purification of Secreted PHB
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Calgary2022_PurificationofSecretedPHB.pdf
Separation of PHB
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Calgary2022_SeparationofPHB.pdf
Nisin Protocols
Golden Gate Assembly
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Calgary2022_GoldenGateAssembly.pdf
Gram Staining
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Calgary2022_GramStaining.pdf
Immobilization of BC to Nisin
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Calgary2022_ImmobilizationofBCtoNisin.pdf
Kirby Bauer Antimicrobial Tests
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Calgary2022_KirbyBauerAntimicrobialTests.pdf
Minimal Inhibitory Concentration
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Calgary2022_MinimalInhibitoryConcentrationProtocol.pdf
Mold Swabbing Procedure
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Calgary2022_MoldSwabbingProcedure.pdf
PDA Plate Protocol
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Calgary2022_PDAPlateProtocol.pdf
Storing Fungi
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Calgary2022_StoringFungi.pdf
Yeast Peptone Dextrose Broth and Innoculation
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Calgary2022_YeastPeptoneDextrosebrothandInnoculation.pdf
Fruit Waste Media Protocols
BC Standing Culture (Seed Growth)
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Calgary2022_fwm1p.pdf
Enzymatic Hydrolysis of Orange Peels
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Calgary2022_fwm2p.pdf
FWM (Modified Media) Protocol
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Calgary2022_fwm3p.pdf
FWM Agar plates
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Calgary2022_fwm4p.pdf
FWM Plating Procedure
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Calgary2022_fwm5p.pdf
Growing BC in HS-FWM
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Calgary2022_fwm6.pdf
DNS Assay Protocol
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Calgary2022_fwm7p.pdf
Orange Pre-Processing Procedure
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Calgary2022_fwm8.pdf
Preparing HS-FWM
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Calgary2022_fwm9.pdf
Sodium Acetate Buffer
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Calgary2022_fwm10p.pdf
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