Cellucoat's toolbox created for NisQ and PHB expression
Food waste is becoming an ever growing and prominent global problem. Hence, the work Cellucoat has done using synthetic biology is critical to tackle the food waste issue. We have designed 9 different parts; 4 of which add the preservation component of our bacterial cellulose (BC) fruit packaging, and the other 5 which serve to alter the mechanical properties of our BC fruit packaging. The development of these multifaceted parts we are able to help future iGEM teams in making an impact by using synthetic biology.
Cellucoat’s parts collection is split into 2 categories; parts made to give our fruit packaging preservative properties and parts made to alter the mechanical strength. Our NisQ collection aid us in purifying nisin using different solubility tags as well as affinity tags such as His-tags or GST-tags. The diverse PHB parts in Cellucoat’s PHB collection contain different ribosome binding sites (RBS) with differing strength in order to increase the production of our chosen protein. Read more on our Part Collections page to read more about our part design.
|BBa_K4437001||Coding||NisQ with a N-terminus 6x His-tag (NisQ-His)(+/-)||Danielle Korsrud, Tithi Bhatt||351 bp|
|BBa_K4437002||Coding||NisQ with an N-terminus NusA solubility factor and 6x His-tag||Danielle Korsrud, Tithi Bhatt||1834 bp|
|BBa_K4437003||Coding||NisQ with an N-terminus GST-NusA fusion solubility factor and 6x His-tag||Danielle Korsrud, Tithi Bhatt||2500 bp|
|BBa_K4437004||Coding||Antimicrobial region of NisQ derived from L. lactis||Danielle Korsrud, Tithi Bhatt||105 bp|
|BBa_K4437500||Coding||A non-functional RBS (for use as a negative control)||Marian Grell, Zainab Hakim||15 bp|
|BBa_K4437501||Coding||Phasin with a C-terminus Hly-A secretion tag, an N-terminus FLAGTM tag, and the DeadRBS (BBa_K4437501) RBS.||Marian Grell, Zainab Hakim||796 bp|
|BBa_K4437502||Coding||Phasin with a C-terminus Hly-A secretion tag, an N-terminus FLAGTM tag, and the BBa_B0030 RBS.||Marian Grell, Zainab Hakim||796 bp|
|BBa_K4437503||Coding||Phasin with a C-terminus Hly-A secretion tag, an N-terminus FLAGTM tag, and the BBa_B0034 RBS.||Marian Grell, Zainab Hakim||793 bp|
|BBa_K4437504||Coding||Phasin with a C-terminus Hly-A secretion tag, an N-terminus FLAGTM tag, and the BBa_B0035 RBS.||Marian Grell, Zainab Hakim||795 bp|