We wouldn’t have been able to complete our project without a great team and our mentors!
XJTU iGEM 2022 would like to thank everyone who helped our team, whether it was big or small.
In the process of brainstorming, we put forward a lot of research direction, and has carried on the grouping to three directions, respectively is the synthesis of new herbicides and degradation of Marine plastic ion channels, and access for strict determination of the capsaicin, after the report, we under the guidance of PI vote decided the project theme of this year, the following members actively involved in the brainstorming, We thank them for their contributions to research on these topics.
Member：Chenyi Lei,Yuezhuangnan Liu,Haiyao Liu,Qiuyu Lu,Jiayi Zhou,Enze Gong,Lianghui Liu,Xiang Li,Zhenxiao Cao,Yibo Yuan,Wenyuan Shao，Wenxi He
Our wet experiments can be divided into four main areas: plasmid construction, expression level detection, product detection and fluorescence detection. Therefore, we have a division of labor and cooperation.The following students overcame many difficulties and did their best to complete the experiment. We thank them for their enthusiasm and seriousness.
We built several models for the project content and data from many aspects, the work mainly includes model design, equation analysis, parameter search and detection, coding and debugging. We thank the following students for their efforts in making the project more authentic.
These team members below were responsible for the design of team logo, team uniform and other aspects, and participated in the production of Promotion Video & Presentation Video, including script writing, art design, dubbing, animation production, video editing and other work.
We have designed a Climate simulator as a matching hardware for our project.
Design：Tingyu Zhou,Xiangsheng Zeng,Xiang Li,Wenxi He
Coding&Debugging:Tingyu Zhou,Xiangsheng Zeng
Construct:Tingyu Zhou,Xiangsheng Zeng
The following members completed the construction of the wiki pages, including the design of the page structure and color scheme, the writing of the code, and the presentation of images and text.
Overall Design:Lianghui Liu
Art Design:Lianghui Liu,Chonghao Hao
iGEM is also a rare research experience, we are very grateful to our PIs and Instructors for their patient assistance.
Professor Tan helped us to determine the final topic in several research directions of brainstorming. She taught us to construct plasmids, optimize the experimental progress, and provided many useful suggestions.She’s also responsible for the application of project funds and the ordering of laboratory reagents.
Professor Lu encouraged us to be bold and innovative, and let us clear the meaning of participating in igem.She also helped us prepare for various activities such as brainstorming; Helped us to complete the project approval and fund application.
Hepled us to polish and perfect our plan of human practice; also broaden the useful prospect of our project to make it benefit more people.
Responsible for the management and teaching of laboratory equipment, conduct safety training for laboratory members.
Responsible for the management and teaching of laboratory equipment.
Professor Chen Kunming is a doctor of science in botany and a doctor of development and cell biology. He is very interested in our project and has given us many suggestions and help. We mainly discussed the reasons why glyphosate has been gradually replaced and the suggestions for improvement of our project.
He talked about whether the production of glufosinate-ammonium analogues reached the lethal dose, which made up the design loopholes of our project. In the subsequent modeling test, we paid special attention to this, and we newly designed EPS synthetic circuit to ensure the effective concentration of herbicide in the soil. In addition, for the evaluation of production cost and application potential
Professor Hu Zhaonong is a professor of the College of Plant Protection, mainly engaged in the research of botanical pesticides. The interview content includes the relativity of weeds, main hazards and comprehensive control measures, which provide new ideas and new content for our education lectures.
Similarly, the professor also pointed out that we need to pay attention to the yield of herbicides, but from different perspectives, due to insufficient concentration, weeds may be in a sublethal state and prone to resistance; In addition, he pointed out that we should try our best to ensure that the synthetic herbicide is L-type. For the above, we will repeatedly test in the hardware later, and this part of work will be shown in the hardware. However, due to limited time, we will optimize the circuit design in the future to ensure that the synthetic herbicide is L-type as much as possible.
We have always had some confusion in the experiment, so we contacted assistant professor, Julius Fredens, at National University of Singapore on October 3. He is an expert in synthetic biology. Previously, we learned that he published an article entitled “Total synthesis of Escherichia coli with a recoded genome” in Nature as the first author, which we were very interested in. We have exchanged a lot with professors. In addition to professional knowledge and project related issues, there are also reasons for professors to choose synthetic biology and their expectations for the future of synthetic biology. After the exchange, we will feed back the results to the experimental group, and be more determined to do a good job in biological fertilizer and spread the dividends of synthetic biology.