Results

Overview

This section describes the experimental work performed during our 4 months internship in the lab. A first part details the construction and characterization of the allergen-expressing strains and the DARPin-expressing strain, as well as our first tries to detect aggregation between the two strains in presence of IgE. A second part presents the troubleshooting of these preliminary experiments, and a third explains the correction of the DARPin strain resulting from this troubleshooting, as well as the new attempts of aggregation. Finally, we present the development of two high throughput screening methodologies (droplets and cytometry) as a first step towards the detection of IgE presence in patient blood.