Here is a summary table of all the parts designed by iGEM Toulouse INSA-UPS 2022 this year. You will find information such as the purpose of the part, its length, the current status (working, failed, cloning successful or in progress), and the RFC compliance (parts with one star cannot be associated with an RFC according to the rules of the restriction sites. However, each individual element constituting these parts can be associated with at least one RFC). DARPin elements mentionned here correspond to the modified version (see Results).
If you want to know more about our parts or if you have any questions, feel free to contact us at igem.toulouse@gmail.com.

Type	Purpose	Accession Number	Diagram	Significance in the project	Length (bp)	Status	RFC
Basic	Expression of Gal d 2 allergen at the surface of an E. coli cell sortable by FACS.	BBa_K4197005		**	3612	Failed	*
Basic	Expression of Ara h 2 allergen at the surface of an E. coli cell sortable by FACS.	BBa_K4197004		***	2868	Failed	*
Basic	Expression of Ara h 2 allergen at the surface of an E. coli cell sortable by FACS.	BBa_K4197022		***	2868	In progress	*
Basic	Expression of Ana o 3 allergen at the surface of an E. coli cell sortable by FACS.	BBa_K4197003		**	2868	Failed	*
Basic	Expression of Ana o 3 allergen at the surface of an E. coli cell sortable by FACS.	BBa_K4197021		**	2868	In progress	*
Basic	Expression of Der p 1 allergen at the surface of an E. coli cell sortable by FACS.	BBa_K4197002		**	3414	Failed	1000
Basic	Expression of DARPin at the surface of an E. coli cell sortable by FACS.	BBa_K4197001		***	2447	In progress	1000
Basic	IPTG inducible system to express DARPin at the surface of E. coli.	BBa_K4197011		***	891	In progress	1000
Basic	IPTG inducible system to express DARPin-sfGFP fusion protein at the surface of E. coli.	BBa_K4197010		**	1614	In progress	1000
Basic	IPTG inducible system to express Gal d 2 allergen at the surface of E. coli.	BBa_K4197009		**	2001	In progress	*
Basic	IPTG inducible system to express Ara h 2 allergen at the surface of E. coli.	BBa_K4197008		***	1359	In progress	*
Basic	IPTG inducible system to express Ana o 3 allergen at the surface of E. coli.	BBa_K4197007		**	1257	In progress	*
Basic	IPTG inducible system to express Der p 1 allergen at the surface of E. coli.	BBa_K4197006		***	1803	In progress	1000
Basic	Brick expressing mRFP1 fluorescent protein with ihfB800 promoter.	BBa_K4197012		**	1578	Failed	1000
Basic	Brick expressing mTagBFP fluorescent protein with ihfB800 promoter.	BBa_K4197013		**	1577	Working	1000
Basic	Constitutive promoter of E. coli.	BBa_K4197014		**	799	Working	21, 1000
Basic	Protein recognising the constant part of IgE.	BBa_K4197019		***	240	In progress	10, 12, 21, 23, 1000
Basic	Egg allergen binding the variable fragment of anti Gal d 2 IgE.	BBa_K4197018		**	1158	In progress	10, 21, 23, 25
Basic	Peanut allergen binding the variable fragment of anti Ara h 2 IgE.	BBa_K4197017		***	516	In progress	10, 12, 23, 25
Basic	Cashew allergen binding the variable fragment of anti Ana o 3 IgE.	BBa_K4197016		**	414	In progress	10, 12, 21, 23, 25
Basic	Cashew allergen binding the variable fragment of anti Der p 1 IgE.	BBa_K4197015		***	960	In progress	10, 12, 21, 23, 25, 1000

Contribution

During our project, we contributed to the improvement of three existing parts on iGEM. We found a new constitutive promoter to express fluorescence in E. coli. We tested it for the red fluorescent protein mScarlet-I (BBa_K2333414) and the blue fluorescent protein mTagBFP (BBa_K592100). We brought too for the OmpA part a crystallographic structure found during our bibliographic research (BBa_K103006).

Type	Purpose	Accession Number	Diagram
Basic	New 3D structure of 46-149 amino-acids of OmpA and 3D prediction of Lpp-OmpA.	BBa_K103006
Basic	Information about the expression with the new promoter ihfB800.	BBa_K2333414
Basic	Information about the expression with the new promoter ihfB800.	BBa_K592100