Project safety has always been a very important part of biological research, especially in iGEM. All experiments in our project are based on the promise of safety. In order to reduce the safety risk of the laboratory, team members have received safety training before entering the laboratory. Each team members attaches great importance to the safety requirements of laboratory work. Throughout the project, team members followed the iGEM safety guidelines strictly.
According to safety protocols, our lab is classified as Biosafety level 1. We received safety training before starting the experiment, learning and following safety protocols, and took care to ensure safety during the experiment.
1.White lab coat, mask and rubber gloves are required for laboratory work;
2.Before starting the experiment, read the instructions carefully and be familiar with the operation of the equipment to avoid the accidents caused by the unfamiliar operation;
3.For the use of sterilization pot we strictly follow the standard process;
4.Most of the reagents used in this experiment are safe;
5.Wear rubber gloves when using reagents to avoid skin contamination with toxic reagent (such as DMSO);
6.Standardize experimental operation to avoid operational errors;
7.The laboratory separates all the experimental materials from dry and wet;
8.The bacteria liquid used in the laboratory is collected, processed and then discarded, which reduces the possibility of laboratory bacteria contamination of the environment.
We also have lab directors who manage our safety affairs, and we have institutions at the college and school level that act as supervisors and supervisors of lab safety.
Firstly, in this experiment, we used Escherichia coli as the chassis for molecular cloning and Saccharomyces cerevisiae as the modified strain. Both strains are widely known and non-pathogenic. Secondly, our strains are auxotrophic. They must be added with specific amino acids in order to grow. Thirdly, the bio-parts used in this experiment are derived from safe plasmids, so they are not unknown segments, reducing the possibility of environmental pollution, pathogenicity and allergies.
In summary, this project can effectively prevent bacteria from escaping from the laboratory and complete the cell apoptosis process to ensure the safety of the entire project and avoid environmental pollution.