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    Parts

    Parts Overview

    In this part page, we uploaded most of the biobricks involved in our project, which is a very meaningful thing. This not only reflects the work we have done, but more importantly, we share the parts with the iGEM community, any team interested in this aspect can apply these biobricks to their projects, as well as carry out transformation and optimization on this basis.

    Parts Table

    Basic Parts

    Name Type Description Length(bp)
    BBa_K4362003 Coding UGI 249
    BBa_K4362004 Coding PmCDA1 594
    BBa_K4362005 Coding dCas9 4101
    BBa_K4362006 regulatory gal promoter 444
    BBa_K4362007 terminator CYC1 260
    BBa_K4362008 Coding SV40 NLS 21
    BBa_K4362009 regulatory TEF1p promoter 348
    BBa_K4362010 Coding hphNT1 1029
    BBa_K4362011 DNA Coding sequence of Micro Venus 5712
    BBa_K4362012 DNA Micro Venus's left homologous arm 26
    BBa_K4362013 DNA Micro Venus's right homologous arm 26
    BBa_K4362014 DNA Coding sequence of Ode To Joy 1257
    BBa_K4362015 DNA Ode To Joy's left homologous arm 20
    BBa_K4362016 DNA Ode To Joy's right homologous arms 20
    BBa_K4362017 DNA Note sequence of Ode To Joy 8
    BBa_K4362018 Composite Micro Venus with homologous arms 5752
    BBa_K4362019 Plasmid Micro Venus-hyg 11104
    BBa_K4362020 Composite Ode To Joy with homologous and note arms 1305
    BBa_K4362021 Plasmid Ode To Joy-hyg 6661
    BBa_K4362022 Composite PmCDA1-dCas9-UGI with NLS label 4989
    BBa_K4362023 Plasmid 42H-gal-dCas9 11733
    BBa_K4362005 Regulatory promoter for VIO-A 809
    BBa_K4362027 Coding produce L-Tryptophan oxidase 1257
    BBa_K4362028 Terminator terminator for VIO-A 400
    BBa_K4362029 Regulatory promoter for VIO-B 786
    BBa_K4362030 Coding produce IPA oxidase 2997
    BBa_K4362032 Terminator terminator for VIO-B 500
    BBa_K4362035 Regulatory promoter for VIO-E 390
    BBa_K4362037 Coding produce vio-e 576
    BBa_K4362039 Terminator terminator for VIO-E 200
    BBa_K4362041 Regulatory promoter for VIO-D 434
    BBa_K4362043 Coding produce vio-d 1125
    BBa_K4362045 Terminator terminator for VIO-D 271
    BBa_K4362047 Regulatory promoter for VIO-C 750
    BBa_K4362049 Coding produce vio-c 1290
    BBa_K4362053 Terminator terminator for VIO-C 240
    BBa_K4362055 Regulatory promoter for URA 238
    BBa_K4362057 Coding produce enzyme to synthetic uracil 804
    BBa_K4362059 Terminator terminator for URA 130
    BBa_K4362061 Coding to gain kanamycin resistance 795
    BBa_K4362063 Coding to gain amicillin resistance 861
    BBa_K4362081 Regulatory promoter for CAT 103
    BBa_K4362034 Regulatory Synthetic promoter-2 110
    BBa_K4362036 Regulatory Synthetic promoter-3 110
    BBa_K4362038 Regulatory Synthetic promoter-4 110
    BBa_K4362040 Regulatory Synthetic promoter-5 110
    BBa_K4362042 Regulatory Synthetic promoter-6 110
    BBa_K4362044 Regulatory Synthetic promoter-7 110
    BBa_K4362046 Regulatory Synthetic promoter-8 110
    BBa_K4362048 Regulatory Synthetic promoter-48 110
    BBa_K4362050 Regulatory Synthetic promoter-50 110
    BBa_K4362052 Regulatory promoter for LEU2 188
    BBa_K4362001 Protein coding sequences produce Chloramphenicol acetyltransferase 660
    BBa_K4362054 Protein coding sequences protein mediating the assembly of a replication
    complex at ori2    		 756
    BBa_K4362056 Protein coding sequences ensure the proper distribution of newly-replicated plasmids
    to daughter cells during cell division     		1176
    BBa_K4362058 Protein coding sequences ensure the proper distribution of newly-replicated plasmids
    to daughter cells during cell division     		972
    BBa_K4362060 Protein coding sequences produce leu 1095
    BBa_K4362062 Terminators terminator for CAT 48
    BBa_K4362064 Terminators Synthetic terminator-1 39
    BBa_K4362065 Terminators Synthetic terminator-2 39
    BBa_K4362066 Terminators Synthetic terminator-3 39
    BBa_K4362067 Terminators Synthetic terminator-4 39
    BBa_K4362068 Terminators Synthetic terminator-5 39
    BBa_K4362069 Terminators Synthetic terminator-6 39
    BBa_K4362159 Terminators Synthetic terminator-7 39
    BBa_K4362072 Terminators Synthetic terminator-8 39
    BBa_K4362073 Terminators Synthetic terminator-9 39
    BBa_K4362074 Terminators Synthetic terminator-10 39
    BBa_K4362075 DNA strictly controllable multicopy 615
    BBa_K4362076 DNA controlling a single copy of the plasmid 220
    BBa_K4362077 DNA control a single copy of the plasmid 251
    BBa_K4362078 DNA Accept the actions of sopA and sopB 474
    BBa_K4362079 DNA cos site from lambda phage 399
    BBa_K4362082 Composite plasmid tightly regulated copy-control 4506

    Composite Parts

    Name Type Description Length(bp)
    BBa_K4362022 Composite PmCDA1-dCas9-UGI with NLS label 4989
    BBa_K4362020 Composite Ode To Joy with homologous and note arms 1305
    BBa_K4362018 Composite Micro Venus with homologous arms 5752
    BBa_K4362082 Composite plasmid tightly regulated copy-control 4506
    BBa_K4362019 Plasmid Micro Venus-hyg 11104
    BBa_K4362021 Plasmid Ode To Joy-hyg 6661
    BBa_K4362023 Plasmid 42H-gal-dCas9 11733