Implementation
In recent years, the field of DNA storage has flourished with the development of high-throughput DNA synthesis and sequencing. Many teams have made excellent achievements. In the 2021 paper from Prof. Yingjin Yuan's team published in National Science Review, it was announced that they had stored a "mother and daughter" video (a testing video commonly used in the field of communications) inside a synthetic yeast chromosome[1].
However, the information we produce every day doesn't stay static, and we always need to constantly change our stored information. For example, the information of our bank account is always changing.
The synthesis, assembly and delivery of larger scale DNA sequences are relatively difficult. What our system can do is that it prevents the time and resource wastes in the process of resynthesizing DNA fragments. With Micro Nuwa editing system and our encoding system, it is possible to achieve large amounts of DNA sequence modification, therefore reach our goal of numerously change what was stored in the DNA sequences.
The synthesis, assembly and delivery of larger scale DNA sequences are relatively difficult. What our system can do is that it prevents the time and resource wastes in the process of resynthesizing DNA fragments. With Micro Nuwa editing system and our encoding system, it is possible to achieve large amounts of DNA sequence modification, therefore reach our goal of numerously change what was stored in the DNA sequences.
Information encryption has always been the biggest concern in the field of data storage. Our project can be the future means for information encryption. No one can understand the real information contained in a fragment of DNA before it is decoded to visualized medium. The information DNA sequences make sense if and only if they were obtained after multi-site editing. However, multi-site editing requires the plasmid expressing gRNA array corresponding to the information DNA sequence. A relation of lock and key exists between these two and without the key — gRNA array, the lock — information DNA sequence — cannot be opened.
We look forward for our sparkle to draw attentions of synthesis biologists and thus achieve advancements in DNA storage modification. Currently, synthesis biologist Prof. Yi Wu has shown great interest in further research, who also wished to take the lead of our team in continuous research of the project.
[1]Chen W, Han M, Zhou J, et al. An artificial chromosome for data storage[J]. National Science Review, 2021, 8(5): nwab028.