Engineering Success
Through iteration, three final programs are obtained in the dry experiment part. The Chinese character base conversion program can convert 3,000 common characters into corresponding base sequences. The image-storage program can store a 35-pixel image within 2,500 bp of DNA sequence, while allowing six different images to be made at a time. A music program can store "Ode to Joy" in DNA sequences of up to 2, 000bp and make a single revision to produce another song, "Auld Lang Syne". In the wet experiment section, the most suitable chassis cells and experimental conditions for multi-site editing were obtained.
1) We planned to develop a program capable of converting 7X5 pixel maps into DNA sequences.
2) The sequence would be introduced into yeasts, and the multi-site editing technique was used to
convert the bases.
3) We planned to develop the decoding system to resolve the changes and get another pixel map.
1) We developed the first generation of photo storage programs. But it took about 2,500 bp to store
such a pixel map, and we planned to store seven-pixel maps. Large pieces of DNA take too long to
synthesize. we developed a second coding system to reduce the length of DNA that needs to be
synthesized. The second encoding system created a new pixel map based on the previous image by
encoding only the pixels that need to be changed, so that the remaining six images total only about
2,500 bp, greatly reducing the length of DNA we need to synthesize.
2) we developed a first-generation decoding program for decoding the sequencing results of
individual
cells.
3) However, there are many unedited sites in a single cell. Not only do we need to get single colony
editing results, we need to synthesize all the strains tested for decoding. So we developed a
second-generation decoding program that can synthesize the sequencing results of five cells。
By the image coding program, we obtained a DNA sequence of a Micro Venus pixel map . with the guidance of dry experiment, we also conducted wet experiments via multi-site editing.
Successfully decoded the modified seven images, and verified our image coding design.
1) We planned to develop a program that can convert music into DNA sequences.
2) By transforming the sequence into yeasts and using multi-site editing system, we planned to
change
the bases.
3) We planned to develop the decoding system to convert nonsense notes into a piece of reasonable
music, such as Ode to joy.
1) We have developed the first music coding program capable of storing the Ode to Joy in a DNA
sequence. Professor Wu Yi, the instructor, thought that the storage density was not satisfied, and
there may be some targets misaligned in double-stranded coding.
2) In order to improve the data density, we believe that it is more advantageous to adopt a
MIDI-like encoding format.
We developed the second generational code according to the underlying logical code of MIDI.
3) Since the second generation coding system was developed too late, we chose to design plasmids
with the first generation coding system.
4) We developed a decoding program.
We conducted multi-site editing wet experiments and We sequenced the plasmid.
Using the decoding program, we successfully converted the encoded sequence into the MIDI format of Ode to Joy which could play on the computer.