This year we have made a total of 32 parts to contribute to the iGEM parts registry including 15 basic parts and 17 composite parts, and improved the characterisation of one existing University of Sydney part (fuGFP (BBa_K3814004), see Contribution page). The functions of our parts range from basic nanobody and fluorescent proteins to GFP-CBD (cellulose binding domain) fusion proteins. We are so excited to show you how much we have achieved in a short amount of time, more information about the parts can be found by clicking the part number linked to their registry pages.
| Part No. | Type | Name | Length |
|---|---|---|---|
| BBa_K4488000 | Coding | Neae: N terminus of 𝛃-intimin for Surface Display in E. coli | 1962 bp |
| BBa_K4488001 | Coding | Anti-GFP Nanobody/sdAb | 397 bp |
| BBa_K4488002 | Coding | Anti-GFP Nanobody/sdAb | 384 bp |
| BBa_K4488003 | Coding | Anti-GFP Nanobody/sdAb | 381 bp |
| BBa_K4488004 | Coding | Anti-GFP Nanobody/sdAb | 374 bp |
| BBa_K4488005 | Coding | Anti-GFP Nanobody/sdAb | 375 bp |
| BBa_K4488006 | Protein_Domain | Flexible Linker (G4S1) x 3 | 45 bp |
| BBa_K4488015 | RBS | RBS | 20 bp |
| BBa_K4488016 | Coding | fuGFPy (Yellow Shifted free-use GFP) | 720 bp |
| BBa_K4488017 | Coding | fuGFPb | 720 bp |
| BBa_K4488018 | Coding | fuBFP | 720 bp |
| BBa_K4488022 | Coding | Modified cellulose binding domain from Clostridium cellulovorans (CBDclos) | 300 bp |
| BBa_K4488023 | Coding | Modified cellulose binding domain from Cellulomonas fimi exoglucanase (CBDcex) | 327 bp |
| BBa_K4488024 | Coding | Modified cellulose binding domain from Clostridium thermocellum cellulosomal scaffold protein A (CBDcipA) | 477 bp |
| BBa_K4488025 | Coding | fuGFP-alanine | 720 bp |
| Part No. | Type | Name | Length |
|---|---|---|---|
| BBa_K4488007 | Coding | Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end | 1044 bp |
| BBa_K4488008 | Coding | Fusion of free-use GFP with CBDcex (cellulose-binding domain) at the C-terminal end | 1071 bp |
| BBa_K4488009 | Coding | Fusion of free-use GFP with CBDcipA (cellulose-binding domain) at the C-terminal end | 1221 bp |
| BBa_K4488010 | Coding | Fusion of free-use GFP with CBDcenA (cellulose-binding domain) at the C-terminal end | 1152 bp |
| BBa_K4488011 | Coding | Fusion of free-use GFP with CBDclos (cellulose-binding domain) at the C-terminal end with a linker | 1089 bp |
| BBa_K4488012 | Coding | Fusion of free-use GFP with CBDcex (cellulose-binding domain) at the C-terminal end with a linker | 1116 bp |
| BBa_K4488013 | Coding | Fusion of free-use GFP with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker | 1266 bp |
| BBa_K4488014 | Coding | Fusion of free-use GFP with CBDcenA (cellulose-binding domain) at the C-terminal end with a linker | 1197 bp |
| BBa_K4488019 | Coding | Fusion of free-use GFPy with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker | 1258 bp |
| BBa_K4488020 | Coding | Fusion of free-use GFPb with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker | 1258 bp |
| BBa_K4488021 | Coding | Fusion of free-use BFP with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker | 1258 bp |
| BBa_K4488026 | Coding | Fusion of free-use GFP-alanine with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker | 1366 bp |
| BBa_K4488027 | Coding | Neae-intimin Construct Displaying anti-sfGFP Nanobody H | 2402 bp |
| BBa_K4488028 | Coding | Neae-intimin Construct Displaying anti-sfGFP Nanobody 3 | 2411 bp |
| BBa_K4488029 | Coding | Neae-intimin Construct Displaying anti-sfGFP Nanobody 6 | 2408 bp |
| BBa_K4488030 | Coding | Neae-intimin Construct Displaying anti-sfGFP Nanobody 7 | 2423 bp |
| BBa_K4488031 | Coding | Neae-intimin Construct Displaying anti-sfGFP Nanobody 2 | 2424 bp |
The parts we have made this year form a broad collection, the collection of fluorescent proteins (fuGFP, fuGFPb, fuGFPy, fuBFP, and fuGFPa) and them fusing with CBD allows for the immobilisation of a wide variety of free-use fluorescent proteins onto cellulose.
| fuGFP variants | fuGFP variants-CBD fusion proteins |
|---|---|
| - | BBa_K4488013 (fuGFP-linker-CBDcipA) |
| BBa_K4488016 (fuGFPy) | BBa_K4488019 (fuGFPy-linker-CBDcipA) |
| BBa_K4488017 (fuGFPb) | BBa_K4488020 (fuGFPb-linker-CBDcipA) |
| BBa_K4488018 (fuBFP) | BBa_K4488021 (fuBFP-linker-CBDcipA) |
| BBa_K4488025 (fuGFPa) | BBa_K4488026 (fuGFPa-linker-CBDcipA) |