We've been working hard inside and outside of the lab for around four
months, and whilst it has been challenging, we've had a lot of fun! Read
more about it below.
Nanobody surface display
Parts design of nanobodies and Neae
fuGFP-CBD
Parts design of fuGFP-CBD
HP and Modelling
Brainstormed session of what we would like to do for the Tahgara
Winter programme
Delegated tasks and set deadlines to different members
Attended information session for science week stall at the
Australian museum
Nanobody surface display
Performed colony PCR on nanobody cloning/transformation products
fuGFP-CBD
Ran SDS-PAGE and fluorescence plate readings to compare
different induction concentrations using cumate
Repeated assay using cotton and compared induced and uninduced
samples of the 4 fuGFP-CBD constructs
Determined optimal induction time by running a time course assay
HP and Modelling
After weeks of trying, OpenMM abandoned as a tool to model the
docking of GFPs and nanobodies, owing to a lack of success and
an overabundance of errors
Nanobody surface display
Tested nanobody binding to sfGFP and fuGFP
fuGFP-CBD
Ran SDS-PAGE of both induced and uninduced of 4 fuGFP-CBDs
tested before, including the insoluble fraction for
fuGFP-CBDcipA (brightest sample)
fuGFP-CBD samples need linker as most of the construct was in
the insoluble fraction