This research aims to find an alternative solution to bacterial inhibition. Currently, antibiotics are popular choices due to their effectiveness against microorganism infection, while their abuse leads to bacterial resistance issues thus posing a huge threat to global health. Antimicrobial peptides (AMPs) are alkaline polypeptides that can inhibit bacterial activity without causing bacterial resistance. For this issue, we developed five types of AMPs based on the following functional genes: Hydramacin-1, Spheniscin-2, Sparamosin26-54, LL-37, and fusion. The target gene was integrated into plasmid pET28a(+), expressing AMPs in E.coli BL21(DE3) competent cell. Besides the development of bacterial inhibition functions, the fusion AMP has antifungal abilities. Because the aquariums regularly replace a lot of water, we hope that the AMPs created can shed light on a more convenient method of fish tank cleaning to save water resources.