Notebook

Lab notes
Wet Lab
Dry Lab
Human Practice
  • June
    2022
  • Sunday
  • Monday
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  • Wednesday
  • Thursday
  • Friday
  • Saturday
    • 29
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    • 1
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  • July
    2022
  • Sunday
  • Monday
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  • Wednesday
  • Thursday
  • Friday
  • Saturday
    • 26
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    • 28
    • 29
    • 30
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  • August
    2022
  • Sunday
  • Monday
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  • Saturday
    • 31
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    • 31
    • 1
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  • september
    2022
  • Sunday
  • Monday
  • Tuesday
  • Wednesday
  • Thursday
  • Friday
  • Saturday
    • 28
    • 29
    • 30
    • 31
    • 1
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    • 27
    • 28
    • 29
    • 30
    • 1
    • 2
    • 3
    • 4
    • 5
    • 6
    • 7
    • 8
  • October
    2022
  • Sunday
  • Monday
  • Tuesday
  • Wednesday
  • Thursday
  • Friday
  • Saturday
    • 25
    • 26
    • 27
    • 28
    • 29
    • 30
    • 1
    • 2
    • 3
    • 4
    • 5
    • 6
    • 7
    • 8
    • 9
    • 10
    • 11
    • 12
    • 13
    • 14
    • 15
    • 16
    • 17
    • 18
    • 19
    • 20
    • 21
    • 22
    • 23
    • 24
    • 25
    • 26
    • 27
    • 28
    • 29
    • 30
    • 31
    • 1
    • 2
    • 3
    • 4
    • 5

2022.06.23

IDT gblock resuspend

A, B, C, D, E, F

Taq PCR

A, B, C, D, E, F

Gel electrophoresis

A, B, C, D, E, F

2022.06.29

Transformation

pSB1A3 in DH5α

2022.06.30

Colony PCR

Pick four strains of bacteria from plate: 1, 2, 3, 4

Gel electrophoresis

1, 2, 3, 4

2022.07.01

Culturing

Pick two from each area: 1-1, 1-2, 2-1, 2-2, 3-1, 3-2, 4-1, 4-2

2022.07.02

Plasmid purification

1-1, 1-2, 2-1, 2-2, 3-1, 3-2, 4-1, 4-2

2022.07.04

Phusion PCR & Gel extraction

A, B

Digestion

A, B

Gel extraction

A, B

Ligation

A, B

2022.07.05

Phusion PCR & Gel extraction

E, F

Digestion

E, F

Gel extraction

E, F

Ligation

E, F

Transformation

A, B

2022.07.06

Phusion PCR & Gel extraction

C, D

Digestion

C, D

Gel extraction

C, D

Ligation

E, F

Transformation

E, F

Colony PCR & Gel electrophoresis

Pick eight plants from each plate A & B
A: three from each II, III, V area (A-2, A-3, A-5)
B: three from each IV, VIII (B-4, B-8)

2022.07.07

Transformation

E, F

Colony PCR & Gel electrophoresis

E => Pick three plants from plate

Ligation

E, F

Transformation

C, D

Culturing

A, B: Three tubes from each: A-2, A-3, A-5, B-4, B-8 (15 tubes in total)

2022.07.08

  • A: His Ubx
  • B: His EGFP Ubx
  • C: PaPilA Ubx
  • D: PaPilA 1-61 Ubx
  • E: His Y4 mRFP Y293Y296
  • F: His Y167 mRFP Y240

Colony PCR

E, F => Pick eight plants from each plate
E: E-1,E-2, E-3,E-4,E-5,E-6,E-7, E-8
F: F-1, F-2, F-3,F-4,F-5,F-6,F-7., F-8

Gel electrophoresis

Eight plants from E, F plate

Plasmid purification

Three tubes from each: A-2, A-3, A-5, B-4, B-8

Ligation

C

2022.07.18

Transformation

C, D, E, F

2022.07.19

Colony PCR & Gel electrophoresis

Pick eight plates from each plate: C, D, E, F
C: C-1, C-2,C-3,C-4,C-5,C-6,C-7, C-8
D: D-1, D-2, D-3, D-4, D-5, D-6, D-7, D-8
E: E-1, E-2, E-3, E-4, E-5, E-6, E-7, E-8
F: F-1, F-2, F-3, F-4, F-5, F-6, F-7, F-8

Culturing

Choose two tubes from each: C-2, C-3, C-7, C-8

Transformation

pSB3K3 in DH5α

Colony PCR & Gel electrophoresis

Pick eight plates from each plate: C, D, E, F
C: C-1, C-2,C-3,C-4,C-5,C-6,C-7, C-8
D: D-1, D-2, D-3, D-4, D-5, D-6, D-7, D-8
E: E-1, E-2, E-3, E-4, E-5, E-6, E-7, E-8
F: F-1, F-2, F-3, F-4, F-5, F-6, F-7, F-8

2022.07.20

Colony PCR & Gel electrophoresis

pSB3K3 in DH5α

Plasmid purification

two tubes from each: C-2, C-3, C-7, C-8

Digest check

C-2, C-3, C-7, C-8

Gel electrophoresis

Wrong result from digest check, so we use:
7/6 Digestion products
7/6 7/8 Ligation products

Culturing

4 mL from each: pSB3K3 B, C, D

Two tubes of 3 mL from each:
pSB1A3 C-11, C-14, D-11, D-12, D-15, E-10, E-14, F-9, F-10, F-11, F-13, F-16

2022.07.21

Plasmid purification

C-11, C-14, D-11, D-12, D-15, E-10, E-14, F-9, F-10, F-11, F-13, F-16

pSB3K3-1, pSB3K3-2

Digest check

C-11, C-14, D-11, D-12, D-15, E-10, E-14, F-9, F-10, F-11, F-13, F-16

Digestion & Gel extraction

A-2, B-8, pSB3K3-1

Transformation

pSB3K3 in DH5α

2022.07.22

Transformation

pSB1A3 A-2, B-8, C-14, D-11, E-14, F-11

Colony PCR

pSB3K3 1~8, C-17, C-18, C-19, C-20

2022.07.23

Culturing

pSB3K3 1~8, C-17, C-18, C-19

Plasmid purification

pSB3K3 1~8, C-17, C-18, C-19

Digestion & Gel extraction

Backbone: pSB3K3 1

Insert: C-14, D-11, E-14, F-11

Culturing

Take eight plants from pSB1A3
(C-14-1, C-14-2, D-11-1, D-11-2, E-14-1, E-14-2, F-11-1, F-11-2)

2022.07.24

Colony PCR

Take eight from each:
A-2, B-8, C-14, D-11, E-14, F-11 (pSB1A3 BL21)

Plasmid purification

C-14, D-11, E-14, F-11 (pSB1A3)

Digestion & Gel extraction

Backbone: pSB3K3 6
Insert: C-14, D-11, E-14, F-11

Buffer W2 Testing

Plasmid culturing

pSB1A3 BL21 A-2-2, A-2-5, B-8-2, B-8-5, C-14-7, C-14-8, D-11-1, D-11-8, E-14-1, E-14-7, F-11-3, F-11-7

2022.07.25

Digestion & Gel extraction

pSB3K3-1, pSB3K3-2, C-14, D-14, E-14, F-11

Digest check

C-17, C-18, C-19 (DH5α)
A-2-2, A-2-5, B-8-2, B-8-5, C-14-7, C-14-8, D-11-1, D-11-8, E-14-1, E-14-7, F-11-3, F-11-7 (BL21)

Ligation

A-2, B-8 (pSB3K3)

2022.07.26

Transformation

A-2, B-8 (pSB3K3 in BL21)

IDT gblock resuspend

G: LL37 Ubx mRFP

Taq PCR & Gel electrophoresis

G

Phusion PCR & Gel extraction

G

2022.07.27

Colony PCR

A-2, B-8, C-14, D-11, E-14, F-11 (pSB1A3)

Phusion PCR & Gel extraction

G

Digestion

G

Ligation

G in pSB1A3

2022.07.28

Transformation

pSB1A3 in BL21

Plasmid purification

A-2-9, A-2-10

Colony PCR

Three from each: A-2, B-8, C-14, D-11, E-14, F-11 (pSB1A3)

Ligation

For start unit: pSB1A3-1, A-2-9-1, A-2-9-2 (Label: E1-C, E1-A-2-1, E1-A-2-2)

2022.07.29

Expression

E1-C, E1-A-2-1, E1-A-2-2

Digest check

E1-A-2-1, E1-A-2-2

Transformation

pSB1A3 in DH5α

Plasmid purification

E1-A-2-1, E1-A-2-2

2022.07.30

Colony PCR

G (pSB1A3 DH5α)

2022.08.01

PCR for primer check

B: His EGFP Ubx

Wash bacterial cells

Bacteria collected every hour from E1-A-2-1, E1-A-2-2

Ligation

C(pSB1A3)

2022.08.02

Transformation

C, G (pSB1A3 in DH5α)

Sonicate

Bacteria collected every hour from E1-A-2-1, E1-A-2-2

Colony PCR

C-1, C-2, C-3 (pSB1A3-1 BL21)

For start unit: pSB1A3-1-1, D-11-9-5, D-11-9-6, F-11-10-4, F-11-10-5 (Label: E2-C, E2-D-1, E2-D-2, E2-F-1, E2-F-2)

2022.08.03

Expression

E2-C, E2-D-1, E2-D-2, E2-F-1, E2-F-2

Plasmid purification

E2-D-1, E2-D-2, E2-F-1, E2-F-2

Colony PCR

Four plants from each C, G (pSB1A3 DH5α)

For start unit: D

Four plants from each B, E

Digest check

E2-D-1, E2-D-2, E2-F-1, E2-F-2

2022.08.04

Plasmid purification

C-2, C-4, G-3

Colony PCR

C-1, C-2, C-3 (pSB1A3 BL21)

Digest check

C-2, C-4, G-3

2022.08.05

Digest check

C-2, C-4, G-3

2022.08.08

Phusion PCR & Gel extraction

C, G

Digestion

C, G

Ligation

C, G

2022.08.09

Transformation

C, G (pSB1A3 in DH5α)

pSB3K3 in BL21

2022.08.10

Digestion & Gel extraction

pSB1A3

Ligation

C, G (pSB1A3)

2022.08.11

Transformation

C, G in DH5α

Plasmid purification

C-1, C-2, C-3, C-4

Digest check

C-1, C-2, C-3, C-4

SDS-PAGE (Protein volume)

The 2, 4, 6, 8, 10, 12, 14, 16 hr from each: E2-D-1, E2-D-2, E2-F-1, E2-F-2

2022.08.12

Colony PCR

Eight plants from C (8/9 product)
Four plants from each C, G (8/11 product)

Colony PCR [Bioart]

BFP, YFP, GFP, RFP (pSB1C3 in DH5α)

2022.08.13

Plasmid purification

C-4, C-7, G-1, G-2, G-3

Digesst check

C-4, C-7, G-1, G-2, G-3

Colony PCR [Bioart]

Two from GFP: G-5, G-6
Six from BFP, YFP, RFP: B-5~10, Y-5~10, R-5~10

2022.08.14

Digest check

G-1, G-2, G-3

2022.08.15

Colony PCR

8/9 C: 15, 16, 17, 18, 19, 20
8/11 C: 5, 6, 7, 8, 9, 10
8/11 G: 5, 6, 7, 8, 9, 10, 11, 12

2022.08.16

Transformation

A, B in BL21

2022.08.17

SDS-PAGE (Protein concentration)

Expression 2

Colony PCR

A, B

2022.08.18

Expression

E3-C, E3-A-3, E3-A-4, E3-B-3, E3-B-4

Plasmid purification

E3-A-3, E3-A-4, E3-B-3, E3-B-4

Digest check

E3-A-3, E3-A-4, E3-B-3, E3-B-4

2022.08.19

Ligation

Backbone: pSB1A3
Insert: G

Digest check & Gel extraction

C-6, C-16

2022.08.24

Phusion PCR & Gel extraction

G

Colony PCR

pSB1A3 (Control)

For start unit: pSB1A3, A, B

Transformation

A, B in BL21

Digestion

G

Ligation

G

2022.08.25

Expression

E4-C, E4-A-3, E4-A-4, E4-B-3, E4-B-4

Digest check

E4-A-3, E4-A-4, E4-B-3, E4-B-4

Colony PCR

G

2022.08.26

Plasmid purification

G

Wash bacteria cells

E4

Sonicate

2, 4, 6, 8, 10, 12, 14, 16 hr of E4

2022.08.27

Digest check

G

2022.08.28

Sonicate

1, 3, 5, 7, 9, 11, 13, 15 hr of E4

SDS-PAGE

Expression 4

2022.08.31

Transformation

DH5α: pSB3K3, A, B

BL21: pSB3K3, A, B

2022.09.01

Colony PCR

pSB3K3, A, B (BL21)

pSB3K3, A, B (BL21)

pSB3K3, A, B (DH5α)

Transformation

pET-30a in DH5α

Expression

E5-Control, E5-A (pSB3K3)

2022.09.02

Colony PCR

pET-30a (DH5α)

Plasmid purification

E5-Control-2, E5-Control-3, E5-A-4

A-4, A-8, B-4, B-5

pET-30a-1, pET-30a-2

2022.09.05

Digest check

E5-A-4, A-4, A-8, B-4, B-5

Digestion

pET-30a-1, pET-30a-2

2022.09.06

Gel extraction

pET-30a-1, pET-30a-2

Ligation

C, D, E, F (pSB3K3)

Taq PCR

A, B, C, D, E, F, G

2022.09.07

Transformation

C, D, E, F (pSB3K3, DH5α)

D, F (pSB3K3, BL21)

Phusion PCR & Gel extraction

A, B, C, D, E, F, G

Digestion

A, B, C, D, E, F, G (pET-30a)

Colony PCR

Control, D, F (pSB3K3, BL21)

2022.09.08

Colony PCR

C, D, E, F (pSB3K3, DH5α)

Expression 6

E6-Control, E6-F-2, E6-F-3

Digestion & Gel extraction

pET-30a-3, pET-30a-4

Plasmid purification

E6-F-2, E6-F-3

Digest check

E6-F-2, E6-F-3

Transformation

N, O, P

2022.09.09

Ligation

A, F, G (pET-30a)

Digestion

pET-30abackbone

Wash bacterial cells & Sonicate

Expression 6

2022.09.11

Transformation

A, F, G (pET-30a, DH5α)

pSB3K3, BL21 plasmid

2022.09.12

Phusion PCR & Gel extraction

pET-30a

pSB3K3

Colony PCR

O, P

Transformation

N, P

Colony PCR electrophoresis check

N, P

2022.09.13

Colony PCR electrophoresis check

P

Transformation

I, J, Q, K, G

2022.09.14

Digest check

C-2, D-2, E-3, F-2 (pSB3K3)

Gel extraction

pET-30a

Colony PCR

D, E (pSB3K3, DH5α)

Ligation

A, B, C, D, E, F, G (pET-30a)

Transformation

L, J, I

2022.09.15

Colony PCR

L, J, I

2022.09.16

Plasmid Extraction

O

2022.09.17

Colony PCR

A, C, D, E, F, G (pET-30a), L

Expression 7

E7-Control, E7-F-3, E7-F-4 (pSB1A3, BL21)

2022.09.18

Expression 8

E8-Control, E8-F-3, E8-F-4 (pSB1A3, BL21)

Digest check

E8-F-3, E8-F-4

Transformation

O

Plasmid Extraction

L

Colony PCR

O

2022.09.19

Digest check

A-3, C-1, C-3, E-2, E-5, F-4, G-2 (pET-30a, DH5α), L

Transformation

pSB3K3 in BL21

2022.09.20

Expression 9

E9-Control, E9-F-3, E9-F-4 (pSB1A3)

Colony PCR

O

2022.09.21

Dialysis

E9-Control, E9-F-3, E9-F-4

Digest check

A-9, A-16 (pET-30a, DH5α), O

Plasmid Extraction

O, R

2022.09.22

Digest check

A-9, A-16 (pET-30a, DH5α), O, R

2022.09.23

Colony PCR

pSB3K3

Expression 10

E10-Control-1a3, E10-Control-3k3, E10-F

Digest check

O

2022.09.24

Transformation

A pSB1A3 to BL21

A pSB3K3 to BL21

Functional test 1&2

F

Colony PCR

pSB1A3 BL21

A

Transformation

R, L

2022.09.25

Expression 11

E11-Control, E11-A

Digestion

pSB3K3

Phusion PCR

G

2022.09.26

Plasmid purification

A

Digest check

A

Transformation

A, G

2022.09.27

Digest check

A

Functional test 3

Control, F

Colony PCR

Control-pSB1A3, Control-pSB3K3, F, G

2022.09.28

Expression 12

E12-Control-1a3, E12-Control-3k3, E12-F

Functional test 4

F

Colony PCR

R, L

2022.09.29

Bacteria Culturing

R, L

Plasmid Extraction

R, L

Digesst Check

R, L

Expression

R, L

2022.09.30

Colony PCR

E, G

Expression 13

Control-3k3, F

Expression 14

Control-1a3, Control-3k3, E

BioArt Protein Mixing

2022.10.01

Digest check

E

2022.10.02

Colony PCR

pSB3K3, F

2022.10.03

Expression 15

Control-3k3, F

2022.10.04

Expression 16

Control-3k3, F

2022.10.08

Expression 17

Control-3k3, F

A: His Ubx

B: His EGFP Ubx

C: PaPilA Ubx

D: PaPilA 1-61 Ubx

E: His Y4 mRFP Y293Y296

F: His Y167 mRFP Y240

G: K592100 (2022 kit, plate 1, well 17O)

H: K592024 (2021 kit, plate 1, well 17C)

I: E1010 (2021 kit, plate 3, well 12M)

J: I13500 (2021 kit, plate 6, well 21E)

K: I13500 (2021 kit, plate 2, well 16P)

L: J04450 (2021 kit, plate 6, well 4C)

M: K1033900 (2021 kit, plate 6, well 5N)

N: E1010 (2019 kit, plate 6, well 12M)

O: B0034 (2019 kit, plate 6, well 1M)

P: I13500 (2019 kit, plate 2, well 16P)

Q: I13500 (2019 kit, plate 6, well 21E)

R: K741002 (2019 kit, plate 2, well 17M)

S: K1033900 (2019 kit, plate 6, well 5N)

05.09 - 05.15

Learn more about Ubx: We want to make elastic fibers that can conduct electricity, finding that the protein Ubx could meet our needs. So we have an in-depth understanding of this protein, including its own characteristics and what kind of protein it can fuse with.

Search for conductive proteins: We searched for many kinds of conductive fibers, and decided to use pilA with better conductivity to fuse with Ubx to meet our thought, which we confirmed the production conditions of pilA and optimized its sequence.

Find other applications: We want to expand the application in other directions and try to make our fibers have various functions, so our goal is to find more usable proteins.

05.16 - 05.22

Learn Ubx spinning method: We are looking for the spinning method of Ubx, and the most suitable temperature and conditions for its spinning.

A new application—antimicrobial peptides(AMPs): We want to connect AMPs(antimicrobial peptides) to aptamer and then combine them with Ubx to make fibers with the ability of killing specific bacteria. However, the necessity of using aptamers is questionable, coupled with its difficult design. Therefore ,we decided to use only antimicrobial peptides for binding to Ubx.

05.23 - 05.29

Screening suitable AMP candidates: We searched for various AMPs and compared their differences to find out candidates fitting our needs.

Find the proteins and conditions that help wound healing: We want to start with wound dressings ,checking the ingredients of wound dressings currently on the market to know the conditions that aid wound healing.Beside,we attempt to improve the disadvantages of currently marketed wound dressings by adding these proteins to our fibers.

05.30 - 06.05

Find other applications: Moving on to other applications, we not only checked the scaffolding conditions for cell growth during tissue engineering, but found chelates that can bind to heavy metals, hoping to be used for water filtration and other purposes.

Meeting with Professor Ming-Chia, Li: We have had a discussion with Professor Ming Chia Li, who specializes in biomaterials. The professor gave us many suggestions and new ideas. Furthermore, We also exchanged knowledge about materials, and the professor allowed us to go into his lab for tensile testing of materials later.

06.06 - 06.12

Optimize the sequence of pilA and AMP

Plan the experimental process

Find other applications

06.13 - 06.19

Confirm appropriate conditions of candidate proteins in human body

Design the functional test of each protein and optimize the sequence

06.20 - 06.26

To know what affects protein conductivity

Design detailed experiment process and determine plasmid species

06.27 - 07.03

Follow-up experiment process design

07.04 - 07.10

Previous protein had problems with functional testing

Find another proteins that are easy to conduct functional test

07.11 - 07.17

New functional protein—lipase

07.18 - 07.24

Follow-up experiment process design

05.09 - 05.14

Learn MATLAB

05.16 - 05.22

Searching for softwares for protein structure prediction

05.23 - 05.29

Searching parameters for RFP expression

05.30 - 06.05

Simulation of how the Ubx protein and E.pili protein connect together through SWISS-MODEL

06.06 - 06.12

Learn MATLAB SimBiology

Expression Model I

  • RFP biobrick
  • derive formula
  • find parameters

06.13 - 06.19

Expression Model II

  • GFP biobrick
  • derive formula
  • find parameters

Expression Model III

  • RFP + GFP biobrick
  • derive formula

06.20 - 06.26

Sorting all data (parameters)

06.27 - 07.03

Learn Autodock, Materials Studio

07.04 - 07.10

Design verification experiment for expression models

Searching 3D printing models from previous teams

Meeting with professor Ming-Chia, Li

07.11 - 07.17

Protein structure prediction

  • predict the structure of Ubx with AlphaFold
  • label binding sites in fusion proteins with Pymol

07.18 - 07.24

Predict the structure of Ubx with I-TASSER

Derive formulas of hydrogel elasticity

Stress analysis with Autodesk Inventor

07.25 - 06.31

Meeting with professor Jhih-Wei, Chu

08.01 - 08.07

Model plan adjustments

  • delete elasticity model
  • maintain expression and protein structure model

Protein mixing model

  • predict the fluorescence brightness over time

08.08 - 08.14

Expression model

  • expression induced with different concentrations of IPTG vs the number of proteins

Protein mixing model

  • wavelength turn to RGB formula

08.15 - 08.21

Protein structure prediction

  • I-TASSER: confidence score checking

Binding region prediction (ANCHOR algorithm)

Protein mixing model

  • design a Scaffold simulating model to change normal picture into fluorescence

08.22 - 08.28

Expression model: experimental verification design

  • design biobrick
  • design experiment steps
  • checking protocol

02.29 - 09.04

Expression model: experimental verification design

Molecular dynamics simulation

09.05 - 09.11

Expression model: experimental verification

Protein structure prediction: AlphaFold2

09.12 - 09.18

Expression model: experimental verification

Protein mixing model

  • convert wavelength to RGB

0919 - 09.25

Expression model: experimental verification

Binding region prediction: ANCHOR

  • analyzing tyrosine binding ability

Protein mixing model

  • scaffold model

09.26 - 10.02

Expression model: experimental verification

Binding region prediction: ANCHOR

Protein mixing model

  • dealing with experimental data
  • run the result of protein mixing model

10.03 - 10.09

Protein mixing model

  • put the interactive result through time on WiKi
  • design interactive result presentation

10.10 - 10.12

Tyrosine binding model

05.09 - 05.15

Establish future plans for HP events.

05.16 - 05.22

Write supporting document in Education Package

05.23 - 05.29

Make lecture slides in Education Package

05.30 - 06.05

6/02 Online meet-up with Professor Ming-Chia, Li

6/05 Online meet-up with team Jiangnan_China

Make board game in Education package (Script)

06.06 - 06.12

Make board game in Education package

06.13 - 06.19

Preparation for The 13th AFOB Regional Symposium (2022 ARS event)

06.20 - 06.26

6/26 Attend The 13th AFOB Regional Symposium (2022 ARS event)

06.27 - 07.03

6/29 Online meet-up with Professor Yu Yuan, Hsiao

7/03 Online meet-up with team Munich

07.04 - 07.10

7/04 Online meet-up with team CUG

7/05 Online meet-up with Huai En, Lu

7/09 Release “Synthetic Biology Classroom” video episode 1

07.11 - 07.17

7/12 Online meet-up with team HKUST

7/16 Release “Synthetic Biology Classroom” video episode 2

07.18 - 07.24

7/18 Online meet-up with team NCHU

7/23 Release “Synthetic Biology Classroom” video episode 3

07.25 - 07.31

7/26 Online meet-up with team KCL

7/30 Release “Synthetic Biology Classroom” video episode 4

08.01 - 08.07

8/02 Meet-up with GEMS_Taiwan

8/04 Second online meet-up with team KCL

8/06 7/12 Release “Synthetic Biology Classroom” video episode 5

8/05 Release workshop poster

Make RPG game in Education Package

08.08 - 08.14

8/13 Release “Synthetic Biology Classroom” video episode 6

8/14 Launch BioArt workshop #1

08.15 - 08.21

8/20 Release “Synthetic Biology Classroom” video episode 7

Make poster of Taiwan SynBio Conference

08.22 - 08.28

8/22,23 Attend Taiwan SynBio Conference

8/25 Launch BioArt speech

8/27 Release “Synthetic Biology Classroom” video episode 8

Make promotion video

08.29 - 09.04

8/31 Education event with Taipei First Girls High School

9/1 BioArt workshop with KCIS_Xiugang

9/3 Release “Synthetic Biology Classroom” video episode 9

Make promotion video

09.05 - 09.11

9/8 Education event with KCIS_Xiugang

09.12 - 09.18

9/15 NYCU_Formosa presentation

Workshop video editing

09.19 - 09.25

9/23 Entrepreneur visit in Hung Chou Fiber Industry

Workshop video editing

09.26 - 10.02

9/28 Bioart phase #3: artwork sharing

Design merchandise

10.03 - 10.09

10/9 Publish BioArt online exhibition

Design merchandise

10.10 - 10.12

BioArt online exhibition