Plasmid Construction
We constructed two basic plasmids pET28a(+)-ChuA-HrtR and pSB1C3-HrtO-cjBlue and finally transferred them together into E.coli sucessful ,synthesizing our target engineered strain to detect small amounts of intestinal bleeding. (Click here to kn ow more)
The plasmid profiles of pET28a(+)-ChuA-HrtR and
pSB1C3-HrtO-cjBlue
After transferring them into E.coli BL21(DE3), we
observed the recombinant plasmid transformed colonies on the LB
culture medium.
Transformed colony on the LB culture medium
We then successfully verified the correctness of
our plasmid by agarose gel electrophoresis:
The a
garose gel electrophoresis
results of the strain containing two plasmids
After repeated experiments, we searched for the
optimal IPTG induction time and obtained the target protein by
ultrasonic cell crusher, and finally successfully observed the band
by SDS-PAGE.
Figure 1. SDS-PAGE result of supernatant of bacteria broken by ultrasound.
Figure 2. SDS-PAGE results of resuspended precipitate after ultrasonic fragmentation of bacteria.
Supporting
Hardware
Device
Collecting and Oscillating Device
Users can collect and treat their own faeces under non-experimental
conditions with our device, and judge whether blood is present in the
faeces by observing the apparent results. (Click here to know more)
Hardware entity
diagram
Biosafety
Box
We use thermal insulation materials for 3D printing, which is conducive to the stable culture of bacteria. The small pore region is
used for culturing the strain, and the large pore region is used for
releasing the reagent tube to ensure the safe transport of the strain.
Biosafety
box