New information for part
Contribution:
Making contributions for future iGEM teams
rightly plays an important role in the competition. Therefore, we aim to add some information to the existing parts and provide
suggestions and references for the future iGEM team. So that
they do not need to repeat the same work or the mistakes of
us.
New information for part:
We supplemented the existing parts
|
BioBricks
|
Type of characterization
|
Codes in the lab
|
|
from literature
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cjBlue
|
|
|
from experiment
|
T7 promoter
|
For BBa_K592011 which was submitted by iGEM11_Uppsala-Sweden
team in 2011. We have supplemented its structure, characteristics, etc.
We believe these information will help future iGEM teams use
it better.
|
name
|
nature
|
absorbance
|
crystalline structure
|
colour
|
|
cjBlue
|
Non-fluorescent pigment
protein
|
610nm
|
1.8 Å
|
blue
|
Besides, we add our characterization results of the
ChuA and HrtR in our engineered bacteria to the part page
of BBa_K3351000 (T7 promoter from pET-28a, submitted by NWU-CHINA-A 2020).
Part BBa_K3351000 was used to express these proteins through pET-28a(+) hosted by
BL21(DE3).
Construction of the heme biosensor system:
Based on the fluorescence signal sensors, we design
a heme-sensitive biosensor that can be used to detect the heme content and visualize the results.
To provide a reference for other teams, we constructed the
pET28a(+)-ChuA-HrtR plasmid and pSB1C3-HrtO-RBS-cjBlue plasmid, and then
transformed the dual plasmids into Escherichia coli to characterize the
heme biosensor system. The pigment gene is located downstream of the
promoter HrtO. When the sensor is induced by heme, the promoter
HrtO will activate the transcription of the downstream pigment
gene.
Gene roadmap:
Hardware:
Membrane filtration device: Considering the cost
and accuracy of the product, we designed a simple separation and
culture device this year, which can detect intestinal microbleeding
under non-experimental conditions.
1.
A filter membrane with a pore size of 5
μ
m was placed at the front end of the syringe to
separate the bacteria from the fecal residue.
2.
The culture device contains dry powder medium, and
E. coli can grow and proliferate after filtering into the culture
device.
3.
Our hardware equipment also includes an oscillatory
heat preservation device, which simulates the laboratory environment
through oscillation and heat preservation, enabling probiotics to
grow, proliferate and express pigments in the culture device. For
details,
see Hardware
click here to haraware
As there are fewer iGEM teams in the Northwest, it
is not convenient to ask for help from nearby teams. To solve this
problem, three teams from Shaanxi
Province built the northwestern part of library in
2019.
We believe this is a good project that not only bring convenience to each team, but also helps other teams build their parts in the future.
This year, we are very happy to see DKU_China
from southeast China
participated in this project.We
have
now
added 26 parts together.
We
will continue to enrich
database in the future
to benefit
more
teams
in China.
Gut Alliance
During the 4th Northwestern China iGEM Meetup,
we found that there were some bottlenecks in the communication between
the teams. So we established the iGEM China Gut Alliance under our
organization with the aim of helping more iGEM teams in the gut region
and giving full play to our value.Our alliance members include NWU-CHINA-A, HS_China, HZAU-China,
LZU-CHINA, LZU-HS-PRO-A, NEU_CHINA, WS_HZBIOX, NJMU-China, UCAS-China,
for a total of nine teams.Since its inception, our alliance has held
many activities.You can click here to learn more about Gut
Alliance.
In the future,
our alliance will continue to serve the iGEM teams
that do intestinal related aspects every year, providing a platform for exchange and cooperation.
And the iGEMers of our alliance can also provide valuable experience
for teams interested in doing intestinal research in the
future.
After the project was established,
we designed a questionnaire and
appealed on as many people as possible to fill it out. The results showed that the public did not pay
enough attention to intestinal health and did not know enough about
intestinal diseases. This neglect can lead to serious bowel
problems. In August this year, NWU-CHINA-A held
a lecture on intestinal knowledge, attended by teams from LZU-CHINA,
HS_China, WS-HZBIOX and NEU-CHINA. After the seminar, the participants
jointly drafted a pamphlet on gut knowledge to help the public
understand gut knowledge and establish the concept of protecting gut
health. We are honored that our manual is available for future iGEM
teams.