In this year's project, we aerobically engineered the obligate anaerobic bacterium Clostridium tyrobutyricum, exploring its synthetic biology pathways from strict anaerobic to adaptation to microaerobic environments.

In this process, we carefully designed, constructed and characterized some of the components by consulting the literature, designing experiments, experimental verification, etc., hoping to help the later teams with the similar ideas.

In the Basic parts section, we designed some of the promoters, protein coding sequences, reporter proteins and terminators which has been shown to be effective in our project, and their different functions were uploaded separately in part pages.

In the composite parts section, some devices are uploaded. At the same time, we uploaded the genetic circuit built for the characterization of the parts previously designed by our team, and the detailed characterization data and results were uploaded to the contribution of the original page at the same time.

In this year's project, we used a relatively novel chassis, Clostridium tyrobutyricum, which has not been commonly used in iGEM, but often used to produce butyric acid.

We hope that the excellent genes found and isolated from this species could be designed as new parts that may help follow-up teams in this regard, so this year we collected some characteristic genes derived from C. tyrobutyricum and uploaded them to the Part Registry.