Collaborate with three school

Hainan U

Hainan island surrounded by the sea, a large number of antibiotics are applied to all stages of the breeding process, and huge selection pressure led to a significant increase in the types and number of drug-resistant bacteria in Marine aquaculture water, the biological occurrence of aquaculture pathogenic disease occurs more and more frequent, the prevention and control of microbiology is becoming more and more difficult. A more serious problem is that aquatic products bred in this way tend to carry resistant ant bacteria, which are spread through the food chain and can directly threaten human health and life safety

Hainan University plans this year to build a platform called the "locked" cripr / cas tool based on synthetic biology methods, aiming to develop a tool that uses crispr-cas13 / 14 to detect Marine microbes with high aluminum resistance accuracy and easy to operate.

Our project carried out the synthesis of phenylethanol and linalool this year, which is similar to the team of Hainan University in lethal gene synthesis. Under the introduction of Li Tianhong, At the beginning of the competition, we met Hainan University through the introduction of the team AD. In the whole process of the competition, we had in-depth exchanges, shared the details of the team establishment, supported each other in case of difficulties, and carried out a number of cooperation.

In the full and pleasant cooperation, we have established a common goal, promoting the development of each other's projects and enhancing our friendship through a series of activities such as communicating Human Practice work, experimental mutual assistance, and making our manuals.

The Partnership was established : 4.30

This year, Li Tianhong, who has rich experience in the iGEM competition, also served as the mentor of HainanU-China and guided JLU-China as a advisor. Under the recommendation of Tianhong, Huang Shuo, captain of HainanU-China, got acquainted with Wang Xu, captain of JLU-China. After having a basic understanding of their respective team structure and direction, the two teams conceived the idea of cooperation with human practice. In the following cooperation relationship, the two teams continued to have in-depth exchanges and gradually established an all-round partnership.

On April 30th, the JLU_China and Hainan_U online party met for the first time. Through this gathering, we got a preliminary understanding of each other's team and project content, and established a partnership on May 16th. We have laid the foundation for further cooperation in the future.

Began joint journal production : 5.31

In the early days of the team, we established a good partnership with Hainan University and launched a long-term communication plan.

After that, we held a joint press release every two weeks, focusing on the cooperation between the two teams in all aspects. The specific direction is: experiment part, HP part, looking for an instructor and so on.

As two iGEM newcomers, we had a lot of problems with new teams in the early days. We believe that many problems are in common, so we recorded many basic problems encountered in the process of team formation and competition, and gave our solutions, and compiled this experience manual, to provide reference and help for the later participating teams.

Began conduct experiment : Early June

In terms of the experiment, we designed and discussed the lethal system (Lethal system) together with Hainan University and Xiamen University. We discussed the lethal gene part together with Hainan University and Xiamen University. The Jilin University team designed a blue light and red light combined with a plasmid that can cause death. The Xiamen University team provided the lethal gene, and the Hainan University team gave blue light irradiation to express the lethal blra protein. In conclusion, with the cooperation and help of the three teams, we completed the experiments of blue light lethal gene.

HAINAN-U helps us with wet experiments, and JLU-CHINA helps each other with dry experiments (predicting DNA and RNA unwinding temperatures under different conditions and base mismatches). In addition, Jilin University has also done DNA unwinding temperature modeling work and death verification other work for Hainan University.

Cooperative lethal system experiment with Hainan University : Early Aug

Our cooperation with the experimental part of Haida began on August 1st. During the half-month period from August 1st to August 15th, we had detailed discussions on the experimental content every two days. The expression conditions, culture methods and experimental steps of toxic proteins were all reflected in the meeting. Hainan U will use PCR to amplify the toxic protein gene, and verify the toxic protein expression through transformation, expansion, protein electrolysis and other processes. In mid-August, we sent plasmids with toxic protein genes to Hainan University.

5.23 : Discussed the topic selection and other topics

The consensus reached by the two teams in this online meeting is that: after the two teams set up their respective teams, the two teams will continue to enrich the members of their respective teams, fully investigate, actively select topics, and determine the themes of their respective entries as soon as possible. At the same time, our two teams plan to establish a long-term communication mechanism, and the two teams will also regularly launch the joint press and push the column to introduce the progress of communication between the two teams.

Late May : HP group cooperated

On the Human Practices part, during this year, we helped each other and improve our human practices. In the continuous progress of the project, in order to expand the influence of the two teams, we carried out the cooperation on wechat public account tweets, and continuously integrated the tweets into the final form a manual, contributing to the popularization of synthetic biology, and let more people know about this relatively new research field. In addition, both teams have opened whitelists so as to invite other teams to share experiences or leave simple comments for the manual. In this process, HAINAN-U has made a lot of sample contributions to our project questionnaire survey, and we have also discussed them together to provide the ideas and concepts of the high school popular science summer camp for education's work.

We think deeply about the difficulties we encountered along the way and plan to compile the iGEM experience manual. On April 30,2022, we held our first meeting to discuss the co-production manual. Since then, we have been in touch and pushed multi-period joint experience sharing on the public account. On May 16, the official account published an article, and the cooperation between the two sides officially began. On May 30, the official account published a joint journal (I) to introduce the experience of contacting mentors of both sides. The Joint Journal was released on June 27th (II).

6.28 - 7.6: initially edited the manual content

Up to now, our two teams have jointly pushed the content of four public accounts together. With the advancement of the tweets and the deepening of the content, we have gradually integrated and completed the version 1.0 manual. In the future, I hope to find more teams to join us on this basis, to share, and constantly improve and expand this experience manual, published in the official community in the form of open source, as a truly help the future igem team to learn, promote the development of igem competition and even the development of synthetic biology manual. In this sense, it is not only a summary of experience, but also a platform for all our teams to exchange experience. At the same time, it connects the past and the future, paving the way and waiting for the communication between the latecomers and the pioneers.

FAFU

Using Novel Coronavirus as an example, FAFU has developed a detection system against pathogenic microorganisms. They established a series of polypeptides, based on the human cell-based receptor, ACE2, through a random peptide library approach. We applied this series of polypeptides to E. coli, giving E. coli the ability to "carry" and "identify" the virus. They want to use these microbes in sewage testing in residential sewers. Asdetermined by quantification the produced fluorescent proteins. We can roughly determine the number of COVID-19 patients in the city.

Our system will automatically submit these reports to the quarantine department. To quickly identify the risks of an outbreak in an early stage. The project of Fujian Agriculture & Forestry University in the 2021 season is related to the synthesis of fragrance substances, so after our title is determined, we contacted the Fujian Agriculture and Forestry Team through the website browsing, and conducted the cooperation in this year's experiment. We not only completed the experiments related to the synthesis of fragrance substances, but also jointly completed the cooperative experiments of lethal genes together. Asdetermined by quantification the produced fluorescent proteins. The FAFU team can roughly determine the number of COVID-19 patients in the city. At the same time, their system will automatically submit the reports to the quarantine authorities. To quickly identify the risks of an outbreak in an early stage.

Experimental cooperation : 2022.7-8

Fujian agriculture and forestry team last year's project is the synthesis of phenol ols, the project outline as above. We communicate with them online, communicate the progress of the project and explore cooperation.

We had a deeply friendly exchange with the team of Fujian agriculture and forestry from July to August 2022.

Gradually communicated in depth : In August

In the experiment, both we and the Fujian agriculture and forestry team used the blue light-induced suicide gene, and we provided guidance for their gene synthesis and sent the transformed bacteria. The Fujian agriculture and forestry team found some deficiencies in our experiment, such as linalool may kill E. coli, and gave valuable improvement suggestions based on the experimental experience of last year.

7.23 : 10 universities online public lecture

In July, the synthetic biology classroom jointly produced by 10 universities, including Tsinghua University, Peking University, Beijing Normal University, Jilin University, Tongji University, and Fujian Agriculture and Forestry University, was launched. This public welfare class closely follows the hot topics of biology competition and the frontier fields of synthetic biology, designs the teaching content, takes the project content of each iGEM team as an example, and explains the principles and applications of various synthetic biology technologies in a simple and simple way.

BUCT

Introduction to BUCT: From our own experience, we think that hair loss is a worthy concern. Many people who love beauty pay more attention to hair volume as much as their face, but in the era of high pressure and fast pace, people on the head skin care will inevitably have omissions. After research, BUCT found that the external environment also plays a key role in the retention of hair, and the scalp microenvironment is prone to excessive cleaning or neglect of management injuries. Even if the scalp and hair follicles do not have obvious disease symptoms, many people still say that they are prone to hair loss and scalp sensitivity. And the excessive secretion of scalp sebum can easily lead to microbial breeding, hair follicle inflammation and odor problems. In fact, the skin of the head is weaker than the skin of the face, the arm, which is not conducive to the healthy environment of the hair follicle scalp environment is a major cause of hair loss, so we believe that the soil of hair growth is worth paying attention to.

BUCT designed a probiotic that consumes fatty acids (Aliphatic Acid, AA), Caffeine (CA) and 2-phenylethyl alcohol (2-PE) on the scalp surface and suppresses the growth of harmful bacteria. BUCT is committed to reducing the bad effects of hair loss in the scalp, while improving the excess of sebum and other problems. AA on the surface of human skin is produced by the decomposition of sebum by bacteria or fungi. AA not only mediates the occurrence of partial hair follicle inflammation, but also produces an odor that further leads to excessive cleaning. We enable probiotics to generate CA and 2-PE while consuming AA. CA can shorten the hair follicle growth cycle and stimulate the hair follicle growth, which has been used in various hair care products. 2-PE is a safe food-grade fragrant phenol substance that can improve the odor problem caused by AA. Hopefully, our design can alleviate the hair loss problems of various diseases or non-disease people by improving the skin surface environment, which means that other people with strong scalp oil secretion, itching and desquamation can also try this unprecedented "skin care method".

Meet : 4.30

When we looked for the team of the same synthetic fragrance material in the Chinese IGEMer exchange group, we found the Beijing University of Chemical Technology team. Their project this year was also about the synthesis of aromatic substances, so our two teams had a deep and friendly exchange.

Experiment Push : 6.17

They studied anti-hair loss substances, which involve the production of some phenol substances in the course of the experiment. We have very same parts experimentally. Together, we used the Adh1 gene.

We encountered the problem of low expression efficiency in the synthesis of phenol ols, so we looked for the same synthesis of fragrance substances in the Chinese IGEMer communication group. Fortunately, we met the Beijing Chemical Industry Team. They studied anti-hair loss substances, which involve the production of some phenol substances in the course of the experiment. We have very same parts experimentally.

In communication, we found that Adh1 was used together, for the former to induce phenylalanine, and KdcA, which induced the bacteria to turn on the Erich pathway. Together, we communicated on how to improve expression efficiency and designed controlled trials.

Establishment of the control experimental mechanism : 7.12

BeiChemical added two genes to the original basis. If their expression results are more ideal, then in order to increase the expression efficiency, we will also introduce the two predominant genes of the Erich pathway, and cooperate with them to complete the experiments. If we have more expression results, it can be judged that the original design of adding the two predene genes will encounter overload problems. Similarly, the north chemistry will be corrected according to our experimental results.

8.10 : Hardware Communication

Wang Zhixin, who is in charge of JLU_China hardware, and BUCT students exchanged the latest hardware design ideas about odor release and the practical operation process of 3d printing in the summer vacation, and exchanged the methods and ideas of 3d printing and modeling.

6.13 : Artist Design Exchange Conference

JLU_China team was inspired by the team emblem design tweet of BUCT official account 5.20, led by Li Changjian, a student of JLU team art promotion, and discussed with BUCT about the design concept of the team emblem and mascot of the two teams. Inspired by BUCT students about the design method of logo elements. Around the end of June, the mascot image of the JLU team was unveiled.

7.1 Topic preliminary exchange

They studied anti-hair loss substances, which will involve the production of some fragrant phenol substances during the experiment. We have very same parts experimentally. Together, we used the Adh1 gene.

We encountered the problem of low expression efficiency in the synthesis of phenol ols, so we looked for the same synthesis of fragrance substances in the Chinese IGEMer communication group. Fortunately, we met the Beijing Chemial Industry Team. They studied anti-hair loss substances, which will involve the production of some fragrant phenol substances during the experiment. We have very same parts experimentally.

In communication, we found that Adh1 was jointly used, the former was used to induce phenylalanine to become the KdcA gene, and the latter induced bacteria to turn on the Erich pathway. Together, we communicated on how to improve expression efficiency and designed controlled trials.

7.20 Inspired by the JLU team's subsequent mascot design

Conclusion

We conducted multiple rallies online, and we discuss the main reasons for this result. In our case, we mainly had in-depth discussions and exchanges with Hainan University. Such as the construction of a plasmid and the expression of lethal genes, and so on. We hope that we will maintain long-term friendly exchanges with the above teams, strengthen the partnership and establish a long-term cooperation and assistance mechanism. They can help each other in experimentation, modeling, HP, and publicity. There is a Chinese saying called "complement each other". We believe that all of our several teams will achieve good results in the 2022 season!