Overview

1. In the inspiration phase of the project, through data analysis, we discovered that there is a problem of "mood sickness" among people. At the same time, we noticed an important area of research in consumer psychology: the role of scent on emotions and how this affects people's behavior.

Therefore, we got the inspiration of the project —— to synthesize different scents through microorganisms to regulate people's emotions, thus helping people to relieve anxiety, reduce depression, remember good times, and experience a spiritual journey about scents.

After several investigations, we initially identified synthetic phenylethyl alcohol as our synthetic targets. It has the scent of rose, which is loved by the public.

Smell can directly affect the limbic system of the brain, which is directly related to emotions.

——《Mystery of Smell》

Human childhood experiences and emotions are always related to the smell, even after decades, when smelling a similar smell again, you can recall the experience and emotions at the time.

——Patrick Hain, German consumer psychologist

2. In the project refinement phase, we refined the project from two perspectives: project value and feasibility analysis.

In terms of project value, we first consulted relevant information to meet the safety and ethical aspects of the value. In terms of social value, we consulted with doctors related to aromatherapy at Jilin University. They believe that our project helps to relieve anxiety and depression. In terms of commercial value, we consulted with the manager of Olaughlin corporation, Li. This communication gave us an insight into the broad prospects of bio-produced fragrances.

In terms of feasibility, we first consulted with the FAFU team, who helped us solve the problem of unexpected E. coli deaths. We also communicated with BUCT team and helped each other to solve the problem of low yield. Finally, we communicated with Professior Boxiang Wang from Shenzhen College Of International Education to solve the difficulties in product validation.

The HP during this period have all been instrumental in our project.

3.In the project outlook phase, we interviewed students from Beijing Film Academy, Northeastern University and Beijing University of Posts and Telecommunications to understand the potential role of our product in 4D/5D movies and VR. We accepted their valuable comments and will use them to improve the project in the future. So that our project will have a more prominent value in warming human hearts, promoting social welfare and making the world a better place.

The overall HP process is summarized as follows:

1. Inspiration and topic determination

1.1 Data analysis

Currently, anxiety is becoming a hot topic of concern. An online survey involving tens of thousands of people across China shows that the public's mental and psychological problems were highlighted during the Newcastle pneumonia epidemic, with about 30% of the people surveyed experiencing sub-health conditions such as depression, anxiety and insomnia.

Our analysis of World Health Organization data shows that only 5% of the world's population is completely healthy, 20% of the population has been diagnosed with a disease by a doctor, and 75% of the population is in a sub-healthy state. As shown in the figure below:

The prevalence of serious mental illnesses such as schizophrenia has not changed much in the last 20 years. However, the presence of anxiety and depressive states is increasing in the sub-population.

—— Lu Lin, Academician of Chinese Academy of Sciences, President of Peking University Sixth Hospital

All the above situations show that people have physical and mental stress that needs to be relieved. So, what can we do to solve this problem? We hope that by producing different scents, we can help people associate with good memories and thus relieve emotional stress.

1.2 Literature research

After reviewing the literature, we found that the neural connection between smell and emotion is so strong that 75% of human emotions are produced by smell. The brain regions that process odor and emotion are located in the same neural network structure that is called the limbic system of the brain. The part of the limbic structure that primarily interacts with the olfactory center is the amygdala. Without the amygdala, which is the location in the brain that governs emotions, we would not be able to process experiential or emotional experiences, express our emotions, or understand or recall emotional events. Functional brain imaging studies show that the amygdala becomes active when we perceive an odor, and the more emotional the response to the odor, the more intense the amygdala activity. No intuitive system other than smell can affect the amygdala, the brain region that controls human emotions, in such a unique and direct way. All of our sensory organs react after thinking, except for odors, to which the brain reacts first and then enters into thinking.

[1] Xuekun Huang, Wenhao Zhou,and Chintai Yang." Olfaction and emotion." Chinese Journal of Integrated Chinese and Western Medicine and Otolaryngology 28.04(2020):315-319.

[2] Hou Huijang. A study on odor and emotion recognition based on olfactory EEG.2020.Tianjin University,PhD dissertation.

1.3 Field study

We distributed questionnaires to our friends around us and online, and returned nearly 1,500 copies. Analysis of the survey data revealed that phenylethyl alcohol is currently very commonly used as monomeric fragrance ingredient, and its rose scent are currently the most popular scents in the survey. The results of our survey are shown below:

It can be seen that those who like rose scent account for 67%, those who like lily of the valley scent account for 12%, those who like lavender scent account for 7%, and other floral scents together account for a total of 14%.

On this basis, we likewise asked people if the scent helped to soothe their emotions, if it brought back unique memories, and tried to ask them to share their stories. More than 70% of people said that anxiety would be helped if they had the right floral scent. A lot of people mentioned that the scent of roses reminded them of their first love and childhood days, and the scent of lily of the valley brought them more fond memories of trips with friends.

Based on the above analysis, we finally chose phenylethyl alcohols, which can produce rose fragrance, as the raw materials for the synthesis, which can help to realize the value of our project - releasing fragrance to relieve people's emotional anxiety and depression, and bring people fond memories.

2. Project perfection

2.1 value analysis

In the value analysis section, we take into account a variety of values, including environmental protection, morality and ethics, life and information security, social and business values, etc.

In terms of environmental protection, the engineered bacteria involved in the project will be strictly isolated from the external laboratory environment during the experimental process. After the experiments are completed, they will be sterilized using autoclaving and dry heat sterilization and then placed in designated experimental waste collection points to avoid GMO contamination of the environment. In daily product applications, we use well-sealed bacterium containment to ensure that bacteria will not leak. We have also designed a good lethal system to ensure that the bacteria will die as soon as they leak out, without causing any safety risks to the outside world.

In terms of ethics, we considered the possibility of addictive risks associated with odors. We then reviewed the literature to understand the mechanism of action of phenylethyl alcohol in humans and concluded that there is no risk of addiction. This is further supported by the fact that these two are commercially widely used monomeric fragrance ingredients.

In terms of life safety, we consulted the threshold values of the selected odor molecules and strictly controlled the amount of odor molecules released. And considering that the release of chemical substances may be suspected of chemical abuse or some indirect effects on human body, the molecules we choose are essentially natural extracts of some plants themselves, which will reduce this effect to a certain extent.

In terms of information security, since the process of recognizing emotions requires the collection of biological information such as expressions and heart rate, users may be concerned about the leakage of personal information. Therefore, we have effectively encrypted the data and used distributed storage. We also require the user to authorize the camera and other permissions before the program can run. Users' personal information will only be stored locally and deleted after the program is finished.

In terms of social value, we interviewed a wide range of interest groups in the hope that our products would meet the expectations of a wide range of people. This was evident in the extensive research we conducted prior to selecting the scent that we wanted to choose a scent that would satisfy the majority of people. Further, we interviewed people from various industries, including medical professionals in the field of aromatherapy, who believe that our product is useful for improving depression .

In terms of commercialization, we talked to relevant manufacturers such as Olaughlin, who believe that our product is competitive enough from the market point of view, as there is no homogeneous product and the commercial concept has high value; from the customer point of view, we interviewed from the customer's point of view, we interviewed our friends and conducted research on the Internet, and concluded that our products are popular enough.

2.1.1 Talk to professors who study AI at Jilin University

Professor Profile:

Mr. Wang Xiaoguang (the man in blue) is an associate professor in the School of Computer Science at Jilin University, a director of the Jilin Robotics Society, a member of the Jilin Artificial Intelligence Society, a member of the Robotics Engineering Professional Teaching Steering Committee, a mentor of the Northeast Normal University High School-Jilin University Science Innovation Talent Joint Training Program; head of the Robotics Dream Workshop at Jilin University; has undertaken or participated in many He has undertaken or participated in many pioneering projects of Chinese Academy of Sciences, projects of Jilin Science and Technology Department, industry-university cooperation projects of Ministry of Education, and projects of Jilin Education Department; he has supervised students to win 15 awards, such as the first prize at the national level of ICAN Innovation Competition, the first prize of Jilin Electronic Design Competition, the first prize of China University Student Computer Design Competition, and the second prize of Jilin University Student Robotics Competition.

Mr. Che Haoyuan (the man in black) is a teacher in the Center for Public Computer Teaching and Research and an associate professor. Research interests: computer application, deep learning. He keeps exploring the reform of practical teaching mode and carries out practical teaching practice activities such as inquiry-based, project development group, and participation-based. Deep learning direction: intersecting with bioinformatics discipline, participating in a national key project and declaring a provincial science and technology project. Computer application direction: cooperate with the National Accreditation Center, mainly researching trace and electronic data identification. Cooperate with enterprises in software development to undertake a number of landing projects. Academic competition guidance: won 2 first prizes, 2 second prizes and many third prizes in national competitions (B category).

Two team members, Shiyao Wang (the person in brown ) and Zhixin Wang (the person in white), communicated with two teachers from the following two parts: AI facial recognition and data transmission encryption.

In the part of AI facial recognition, at first we used convolutional neural network based on open source expression data for face training and thus human judgment emotion, but could only achieve 62% recognition accuracy. After discussions between Zhixin Wang and Xiaoguang Wang, we came up with a solution: we next switched to a better dataset for training, using a combination of capturing for the user's own facial features and database assisted reference, using the user's own features as the key input reference, and retesting the improved process. The logic of this solution is that, based on the traditional machine learning process, a better dataset is needed as a sample for machine learning, and our initial data is relatively coarse, and it is difficult for the machine to rely on such data to determine the expressions and emotions of real people.

Mr. Haoyuan Che is recently working on a project related to emotion recognition in mice, but compared to mice, people's complex emotions may be subjectively or objectively passively masked. For example, when they are sad, they may pretend to be calm, or consciously and deliberately make expressions that are different from their actual emotions. This all brings new challenges to our system for effective recognition. The exchange with the teacher inspired us to use a common device, the smart bracelet, to assist us in judgmental recognition. Use the bracelet to achieve targeted capture of heart rate, especially to match for special peak change situations. In order to realize the combination of facial recognition and heart rate, so as to improve the accuracy of the judgment and avoid the possibility of certain mis-touch judgment. So we designed a bracelet user's heart rate for detecting auxiliary input with reference to sports bracelets and other related devices. Eventually, we constructed a new comprehensive judgment system combining heart rate recognition and facial recognition for bracelets.

In terms of user information security, initially we wanted to replace the data acquisition method for security protection, but this method would actually acquire personal information just the same, only the amount of information is different. Further, inspired by Mr. Che Ho-Yuan, we encrypted user parameters during data transmission, using 1024-bit RSA encryption system, and will not save public and private keys locally and automatically destroy them at the end of program operation, thus realizing more stringent privacy protection.

2.1.2 Talking with doctors and scholars who study brain diseases at Jilin University

Our team member, Chanjuan Jin (far right in the picture), talks with a professor at Jilin University who studies medical aspects of the brain. They believe that if our project can produce a corresponding scent, then the specific scent should have an effect on people's emotions.

Scientists are still working tirelessly on how scent and mood interact with each other. Some studies have shown that when the mind is disturbed, the smell of roses can calm the mind; when the mood is low, the smell of fresh apples or reeds can make people feel better; and the smell of lavender can increase a person's trust when interacting with others. Different smells directly affect our emotional perception through the sense of smell, especially if we perceive a certain smell with strong emotions, then the next time we smell that smell, it will trigger a more pronounced emotional response.

They encouraged us to do a good project and inspired us to try to insert an odor molecule fragment into an E. coli to regulate emotions by inducing odor production in the bacteria through light. This led to the development of a blue light module, where blue light irradiation causes E. coli to secrete phenylethyl alcohol.

2.1.3 Communication with Manager Li Peizhi of Olaughlin Corporation

Above is a picture of our meeting with Olaughlin's manager Lee.

Q: we have previously learned that O'Laughlin is a widely acclaimed company in the field of fragrance production, and you are also a very experienced practitioner in the fragrance industry, so it is a pleasure to be able to interview you today. We would like to know the general situation of the current mainstream flavor companies and whether there are any commercial applications for the production of flavors by biological means, what the future prospects are, and whether they are competitive compared to the current mainstream methods.

Answer : When it comes to the flavor industry, I have always been engaged in the work of flavor, I first worked in China's first flavor company Peacock flavor, that may be a company established in 1950 years. I worked in this company after I graduated from university. Later, this flavor company was acquired by a Hong Kong-owned enterprise Huabao. The flavor industry is mainly divided into three directions: edible, daily chemical and tobacco flavors. At that time, I was working in his edible department. At present, the company I work for is called Olaughlin, which is a U.S.-invested fragrance raw material enterprise, so this company is mainly making monomeric fragrance raw materials. In the area of monomeric fragrance ingredients, we focus on synthesis.

Regarding the commercialization of bio-incense, this is actually a very important area at present. Our industry has already started to use synthetic biology to produce some higher value natural fragrance raw materials. For example, major flavor companies such as Givaudan and Firmenich are laying out this field. Biological way to produce incense in commercialization is actually a relatively large application, the prospect is very good, our industry is very optimistic about this aspect. After all, the production of natural fragrance materials is often the same as agricultural production, depending on the sky. Its yield and harvest will often be subject to uncertainty, so often its price fluctuates greatly. So using this kind of engineering bacteria to do synthetic biology to produce incense, in terms of high-value incense raw materials, is very competitive.

Q : Our project device wants to produce pleasant odor substances, and after preliminary research, we are currently mainly producing the phenylethyl alcohol, and would like to ask if there are other odor molecules that can be considered, and whether fragrances can have some effects such as modulating human senses and therapeutic aspects?

Answer : Your device is mainly to produce pleasant substances ah, you now choose phenylethyl alcohol. It has pleasant and comfortable smell. Currently known fragrance raw materials, now in the flavor company inside use, there are more than two thousand kinds, commonly used words have more than 1000 kinds, these have their own special formula. Currently, more scents are used . For example, we have some scents like jasmine . There are rose scents . Perhaps you can try.

If we look at the raw materials of the single fragrance, there is not much research on regulating human sensory therapy. Aromatherapy probably uses more natural essential oils. What you have formulated should not be a natural essential oil, but a natural essential oil is a compound. It may have dozens of hundreds of individual fragrance raw materials, how the specific role is very complex. So that the aromatherapy piece may need to study more thoroughly. Some monomeric fragrance ingredients may also have an effect on the aromatherapy.

Q : We hope you can give us some suggestions on the commercialization process of our product, such as the main audiences we can target or what gaps there are in the project that need to be added.

Answer : I think your overall idea is still very good. At present, the fragrance companies mainly make their perfumes and fragrances into fragrances. These fragrances have many applications in many scenarios . For example, in hotel lobbies or special scenarios, we will promote some fragrances, which should be similar to yours. Your choice is single fragrance materials. I think the regulation of human sensory and therapeutic aspects is certainly effective, but this aspect of the study may not have a more mature research conclusions, but if it is in the fragrance, there have been many mature research conclusions.

From the perspective of product design, I think the consideration is still relatively adequate. But only through facial recognition of emotions may be subjective performance factors, it is best to add a corroborating verification. Like I asked my friend before, they are doing a test of the role of fragrance on emotions, a university in Hong Kong is doing through the nuclear magnetic to test. MRI can capture the changes caused by different fragrances very accurately. But the equipment is too complicated and not light enough for hospitals or scientific research.

Then perhaps we can detect elements such as heart rate through a similar sports bracelet and thus test it? This way, the device is not very big.

Answer : The bracelet should basically also have a certain effect on your design. I think it's still a pretty good choice . The market should also be more reliable technology, you can try.

Q: Do you think that the chemicals produced in our project have any other effects on humans compared to other fragrance products? What else can we do in terms of safety?

Answer : For other effects on the human body, there should be a lot of scientific studies readily available on some of the effects of monomeric incense ingredients on the human body. The fragrance you picked should also have a lot of information, you can go to check. Safety, in general, we are in the fragrance part, in fact, there is a human use of the threshold, required within a fixed range is relatively safe. Just like one of our edible spices. Then the amount added may require no more than a few parts per thousand, both to express the flavor of the product a characteristic taste, and then will not have a relatively large burden on our kidneys. I think the safety aspect, in fact, or to check its a simple threshold, should also have some ready-made data to support.

Q : If we want to propose a product development plan with a realistic time plan, considering resources and risks, do you have a recommended business case that we can refer to?

Answer : I think in fact, your piece, from engineering bacteria to fully controllable production of phenylethyl alcohol, its one of your more core things. For other face recognition, blue light induction and bracelet, there may be some more mature technologies in the market for reference.

In terms of marketing, it seems that there are not many products like yours. At present, for example, in order to sell incense raw materials, fragrance, made it into a certain fragrance, and then through some timed spray or capsule, put in some environment, this is already very mature, and more. But you do this according to human expressions and emotions, I have not seen in the market, so I think this piece is very innovative. There should be a lot of people interested in your investment in commercial promotion. Overseas, mainly in places that need to cover some smell, or need to create some taste, like I just cited, we usually see the most, like some hotel lobbies, etc., they customize some of their hotel characteristics of the aroma, so that once you enter the hotel, you can feel the atmosphere he wants to create. There are many companies that have done this very well, so if you are interested in the later stages I can recommend it to you.

Q : We are currently doing fragrance production mainly in an in-house laboratory environment . The laboratory equipment and resources may have a relatively high cost, but the traditional factory approach may have reduced the cost to a very low level, so I would like to ask, currently say those companies that can achieve synthetic biology fragrance production, how much can they reduce the cost?

Answer : In fact, our company is also currently doing the production of fragrance through synthetic biology. Our product is mainly round pomelo ketone. The starting material of this product is Valenciennes, which is a kind of citrus extracted from citrus oil after the terpenes inside. The smell of this substance is the smell that comes out when we peel the grapefruit, and it is used in some very good drinks in large quantities. But this product is still quite limited due to the upstream raw material restrictions, so now there are many companies that have made it through the fermentation method or engineering bacteria.

The cost of this stuff before, round naringenone, different content price, there is a 98％ content, basically is about twenty-seven thousand eight hundred a kilogram. And we do the chemical through synthetic biology, probably now the cost is only about 13,000 to 14,000. So the price of this product is basically knocked down by half and very consistently and steadily can be made. So it seems to have reached very good commercial value.

However, the product you have picked so far, because it is a relatively low unit price, so if you go to prepare it now, the cost may indeed exceed its current value. But you can find a lot of thousands of fragrance raw materials with very high unit price, such as Bulgarian rose, which is now sold in China for 100,000 yuan a kilogram, and there are some key rose raw materials in it. If you can make it through synthetic biology, then. It can have a very high commercial value.

Q : If we currently form our project into a saleable commodity, we may focus on two highlights, one is that it can be used as an aroma box that can release scent under light adjustments; the other is that it will make intelligent scent adjustments based on face recognition. However, from the laboratory's point of view, there may be no way for us to reduce its cost, do we need to consider switching to some of the scent molecules inside?

Answer : I think it still depends on your market demand. On our side, we are trying to solve some existing problems, such as unreliable sources or very high selling prices. But on your side, there are no such problems, you just have to choose the scent that is pleasant to the human. For these odor molecules, it doesn't have to be something of very high value. There are also lower value things that can achieve this effect. So on your side, it's mainly this business concept that's worth more. Your focus is different from our desire to reduce costs.

Q : What do you think we can still improve?

Q : A：I think you can add some parts such as lights or music, which can better help people relieve their emotions and create an atmosphere. You can even add private customization or special custom scent boxes for certain hotels with specific lighting and music.（Inspired by this, we subsequently added music and lighting components to the hardware）

2.2 Feasible analysis

2.2.1 Exchange with FAFU Team

We found that in the process of advancing our project, we encountered the phenomenon of autolysis of E. coli bacterium. After the interview with FAFU team(Screenshot of the conference as above), we solved the problem of toxic effects of phenylethyl alcohol on E. coli and achieved experimental advancements and breakthroughs. The following picture shows the advice that they gave us:

2.2.2 Exchanges with HainanU Team and XMU Team

Above is a screenshot of our lethal system design and the three teams meeting together.

In the design of the lethal system for this year's project, the BlrA and pBLind-EL222 systems were involved. After reviewing previous years' entries and the iGEM website, we found that the XMU team had used these two systems in season 2021.To resolve the difficulties encountered in obtaining the target genes, we reached out to the XMU team for help.

XMU team presented us with blrA_pCC-1 plasmid. In addition to that, we both discussed and exchanged ideas about pBLind-EL222, a blue light induction system, and the seniors of XMU team 2021 season provided us with detailed guidance on the design of the lethal system.

After communication and discussion with the HainanU team, we redesigned the experimental protocol, and based on the lethal gene provided by XMU, we designed the plasmid for simultaneous irradiation of lethal blue light. However, due to the Changchun epidemic, HainanU assisted us to conduct the experiment of blue light irradiation alone to change the lethal blra protein.

XMU team presented us with blrA_pCC-1 plasmid. In addition to that, we both discussed and exchanged ideas about pBLind-EL222, a blue light induction system, and the seniors of XMU team 2021 season provided us with detailed guidance on the design of the lethal system.

2.2.3 Exchange with BUCT Team

Above is a screenshot of our meeting with the BUCT team and the experimental design of the two teams.

We encountered the problem of inefficient product expression in the synthesis of fragrance phenolics, so we searched for a team in the China IGEMer exchange group that also synthesized fragrance substances. Luckily, we came across the BUCT team. They are studying anti-hair loss substances, and the experimental process will involve the production of some fragrance phenolic substances. We have the very same part in our experiment.

During the communication, we found common use of Adh1 and KdcA genes, the former used to induce phenylalanine to phenylpyruvate and the latter to induce bacteria to open the Ehrlich pathway. We communicated together on how to improve the expression efficiency and designed a control experiment.

BUCT Team added two genes to the original one. If their expression results are more satisfactory, then to increase the expression efficiency, we introduce the same two Ehrlich pathway predecessor genes and cooperate with them to complete the experiment. If we have more expression results, then we can judge that the original design of adding two predecessor genes will encounter the problem of overload. Again BUCT will make corrections with reference to our experimental results.

2.2.4 Communicate with Professor Wang Boxiang of Shenzhen College Of International

Above is a screenshot of our meeting with Professor Boxiang Wang from Shenzhen College Of International Education

Regarding the effect of hydrogel on bacterial growth, Mr. Wang Boxiang pointed out that there may be some effect due to the difference of dissolved oxygen between the inner and outer layers of hydrogel, and we can conduct relevant research on this aspect.

We discussed the issues related to how to argue that linalool has effects on human mood and asked questions about the determination of the products in the meeting. Instructor Bo-Xiang Wang suggested us to validate the products by gas chromatography and mass spectrometry after we got them. It was also suggested that we perform independent validation by blue light first.

In addition, Mr. Wang gave us the experience of making terpene aromatic compounds.

3. Prospect

3.1 Communicate with students from Northeastern University and Beijing University of Posts and Telecommunications about the role of our project in VR

Above are photos of our team leader Xu Wang and students from Northeastern University and Beijing University of Posts and Telecommunications conducting VR experience。

After the project presentation and some careful discussion, the following five points were finally summarized as requiring attention:

1. Scent rendering is primarily a secondary function in VR, so perhaps the cost aspect will be looked at in related commercialization.
2. A scent box that releases too few scents may have too narrow a range of application. At least 5-6 kinds, 14-15 kinds are best. Or give each player a private custom scent box.
3. Consider the safety of players.
4. Face recognition and bracelet part if used in the horror game can be used as a device to prevent overreaction.
5. It is important to consider how long an odor box can be used. Whether the bacteria inside can survive in the long term.

Overall, in the current VR game field, developers mainly focus more on the main senses such as vision and body movement, and smell is not the main optimization direction for the time being. But it can be added later, and I believe it will definitely bring more extreme experience sense to VR games.

3.2 Communication with a Beijing Film Academy student about the role of our project in film

Above is a screenshot of our chat with a student of Beijing Film Academy

Q : Our synthetic biology team has developed a scent box that can recognize the emotion of a person's face based on AI, and then control the corresponding bacteria to release soothing gases. Perhaps this product could be of value in the movie field, such as releasing the scent of the corresponding plot to the audience while the movie is playing. I wonder if we could hear your suggestions on this?

A : Ha ha, that's interesting. Now some 4D and 5D movies seem to have this design.

Q : How about using it as an emotional support actor?

A : I do think it's an interesting point, but it might be a little difficult to apply? For example, you can hardly measure what kind of scent can have what kind of effect on the actor. Even if it smells right, it doesn't mean that the actor can play well? It feels impossible to quantify.

A : Well , what you say is indeed a problem, we follow up to see if we can improve. Maybe go for a personalized scent for the actor. Thank you very much for your reply!

4. Summary

Throughout the project's advancement, HP was present throughout.

Initial> social research, data analysis and text research inspired us and determined the initial expectations of the project. These initial studies fully explained the background and rationale of the project and provided invaluable support for the gestation of this project.

During the project refinement phase, each interaction with various stakeholders provided us with valuable suggestions for improvement, which we incorporated into the project to continuously improve and optimize our project. They helped us to break through the difficulties in our experiments, inspired us to add parts of the bracelet, seek new routes for project improvement, etc. These efforts ensure that our projects are responsible and practically beneficial to the world.

They also support the full realization of the project's multiple values, including ethics. We constructed the final design of the project and verified the level of completion by prioritizing safety values, ethical values, and then other values based on technical feasibility and recommendations obtained from the HP.

We chose to compromise temporarily on some of the commercially valuable improvements that cannot be achieved with current technology. This may only satisfy most of the initial expectations. Therefore, during the development and outlook phase of the project, HP's follow-up communication inspired the direction and approach for future upgrades and improvements to the project. In this way, we achieved a perfect closure of expectations and initial design.

At the same time, we have documented the whole process in HP in detail, which we believe will be a great reference for many teams doing similar directions involving synthetic biology fragrance production work.