7/9
Ice-breaking and self-introductions.
Designing the team name, team logo, and the products we are ordering for charity selling in the near
future.
7/10
Wet group:
Lab safety training.
FoxO1 gene PCR (failed, insufficient yield).
Dry group:
Finishing the buying of all products on online suppliers with low bulk buying prices.
Brainstorming the questions that can be included, potentially, in the online questionnaire.
Starting the writing of online articles by first assigning clearly the labor division.
Designing the offline interview question booklet.
7/11
Wet group:
Cultivate HepG2 cells in MEM-FBS liquid culture.
CR to amplify PGL3 -3IRF, SV40, and FoxO1 genes.
Electrophoresis & validate the correct fragments (1988 bps).
Exaction of the hFoxO1 DNA fragment from the agarose gel.
Dry group:
Offline interview
Learning and starting the preparation of Education and Communication
7/12
Wet group:
Transfer HepG2 cells into new MEM-FBS culture.
Take a sample (20ul) to count the density of cell culture.
Dry group:
Business Plan Workshop and brainstorming every part of the business plan.
Distributing the work to three dry groups.
Finishing the interview conclusion writing.
7/13
Wet group:
Repeat FoxO1 DNA PCR.
Plasmid transfection using LipoFiter 3.0 kit.
Set up 48 well plates, 12 positive control & 12 experimental groups.
Add AS1842856 & compound 355 with varying dosages.
Dry group:
Writing BP first draft and finishing it at midnight.
Interview with Mr. Lu Jian, the first expert we reached out to.
Learning Collaboration and preparation for the collaboration with ATCG Beijing Group.
7/14
Wet group:
Test protein activity using Dual-Luciferase Reporter Assay System.
Isolate cells lysate using lysis buffer (both AS1842856 & compound 355).
Verify the expression of hFoxO1 by Western blot.
Add Firefly & Renilla luciferase to cell lysate solution.
Test luciferase activity in a microplate reader.
Dry group:
Refining the first business plan draft.
Collaboration with ATCG group.
Writing conclusion for the ATCG collaboration.
Interview with Mr. Lu Chen, the second expert we reached out to. Discussion with Mr. Zhang, the business
angel we reached out to due to his unique perspective as a business investor.
7/15
Wet group:
Data collection & analysis.
Verification of the construction of plasmids.
Prep glucose & protein activity test groups: set up 18 well plate
6 positive control & 6 experimental groups, each adding 1ul of glucose solution
Add varying dosages of AS1842856 (positive control) & compound 355 (experimental group)
Dry group:
Education sharing conference
Collaboration with the Shanghai SHSID team.
Writing conclusion for this time collaboration.
Interview with Mr. Ye Mao, our third expert interviewee.
7/16
Wet group:
Test glucose & protein activity of both positive control groups and experimental groups.
Add glucose & protein mixture.
Detect the intensity of luciferase using a microplate reader.
Data collection & analysis.
Presentation preparing.
Dry group:
Offline Fundraising.
Refining the second edition of our business plan.
7/18
Learning about wiki writing and preparing for gathering the materials.
We are going to use the wiki page for manifestation.
Making promotion video
Learning about public speaking skills.
Finishing the shooting of our formal pictures.
7/19
Writing Wiki and gathering the materials.
Making the promotion video
Finishing the last conclusions of the items we done during the days.
