Safety

Rules applied

• No eating, no drinking, no smoking, no makeup, no contact lenses, no open shoes are allowed
• Each member must wear a lab coat, closed shoes, gloves
• Each member must have been trained by the supervisor
• All work surfaces must be decontaminated when finished or when necessary (spill/splash) with ethanol 70% and phagosoft
• The access to the laboratory is controlled
• The hands must be washed before and after experiments
• Sharp objects must be handled safely (needs, scalpels, pipettes, broken glassware)

Chemical substances used

Here is the list of chemical substances used by our team during our project that present a risk. To manipulate, each member was equipped with a lab coat, gloves, security glasses, long pants and closed shoes. Some substances were manipulated under a chemical flow hood.

The following table is a list of hazardous chemicals used by our team during the iGEM competition. To handle them, each member of the lab team was equipped with a lab coat, gloves, safety glasses, long pants and closed shoes. Some substances were handled under a chemical flow hood.

Chemical substance	Related risk [1]
Chloroform	Serious health hazard Acute toxicity
Ethidium Bromide (CMR reagent)	Serious health hazard Acute toxicity
Bleach	Corrosive Hazard environmental
Ethanol	Flammable
Ampicillin	Serious health hazard
Kanamycin	Serious health hazard
Calcium chloride	Health hazard
Isopropanol	Flammable Health hazard
Iodine	Health hazard Hazard environmental
Potassium Iodine	Serious health hazard
TAE 50X (CMR reagent)	Health hazard
TEMED	Corrosive Flammable Health hazard
β-mercaptoethanol	Serious health hazard Corrosive Health hazard
Sodium dodecyl sulfate (SDS)	Corrosive Flammable Health hazard
Ammonium persulfate	Serious health hazard Health hazard hazard oxidizing

Table 1: Chemical substances used by our team during the project

Waste Management

Each waste must be thrown correctly in order to not impact the environment nor humans. All liquids/contaminated material that was in contact with bacteria/chassis must be decontaminated/inactivated in bleach during 12 hours in order to never end up in residual waste. It must be thrown in bins for biological/infectious waste and then incinerated.

CMR liquids must be put in a canister for CMR liquid waste.

Acid solutions must be thrown in a canister for acid waste.

Basic solutions must be thrown in a canister for basic waste.