Experiments

Please find out below the list of the protocols that we conducted:

Potency of DNA using QubitTM from ThermoFisher

Electrophoresis (Gel preparation and migration)

Precipitation of DNA with isopropanol

DNA miniprep

Digestion with restriction enzymes

Gel Extraction by using QIAquick Kit from Qiagen

Ligation protocol using NEB reagents

Competent bacteria

Transformation of competent bacteria

Polymerase Chain Reaction

Preparation of miniculture

Preparation of Petri dishes

Colony PCR

Plate Nile red staining

Assessment of the production of D-Lactate by using D-Lactate Assay Kit from Sigma Aldrich

Preparation of M9 minimal media for Escherichia coli

Electrophoresis of proteins (SDS-Page from bacterial cells)

References

  1. Elbing KL, Brent R, 2019. Recipes and Tools for Culture of Escherichia coli. Curr Protoc Mol Biol. 25(1)
  2. Merk, 2022. D-Lactate Assay Kit. Available at:
    https://www.sigmaaldrich.com/FR/fr/product/sigma/mak336 (accessed on 10/06/2022)
  3. New England Biolabs, 2022. Ligation Protocol with T4 DNA Ligase (M0202). Available at:
    https://international.neb.com/protocols/0001/01/01/dna-ligation-with-t4-dna-ligase-m0202 (accessed on 09/24/2022)
  4. New England Biolabs, 2022. Monarch® Kits for Plasmid Purification. Available at:
    https://international.neb.com/monarch/monarch-kits-for-plasmid-purification (accessed on 09/24/2022)
  5. New England Biolabs, 2022. Protocol for Q5® High-Fidelity 2X Master Mix. Available at:
    https://international.neb.com/protocols/2012/12/07/protocol-for-q5-high-fidelity-2x-master-mix-m0492 (accessed on 09/24/2022)
  6. Promega, 2022. Long-Range PCR: GoTaq® Long PCR Master Mix. Available at: https://france.promega.com/products/pcr/taq-polymerase/gotaq-long-range-pcr-master-mix/?catNum=M4021#protocols (accessed on 09/24/2022)
  7. Promega, 2022. Wizard® Plus SV Minipreps DNA Purification Systems. Available at: https://france.promega.com/en/products/nucleic-acid-extraction/plasmid-purification/wizard-plus-sv-minipreps-dna-purification-systems/?catNum=A1330 (accessed on 09/24/2022)