Overview
In this year, our team submitted 3 protein expression composite parts, Cas13a (BBa_K4223019), Cas14a (BBa_K4223008), and Csm6 (BBa_K4223018), and developed different validation experiments to determine if the different proteins could each play a role in our project.
Table
| Part | Part number | Part type | Basic/Composite | Designer | Length |
|---|---|---|---|---|---|
| Existing Parts | |||||
| T7 Promoter | BBa_I719005 | Regulatory | Basic | Imperial 2007 | 23bp |
| T7 terminator | BBa_K731721 | Terminator | Basic | Giacomo Giacomelli, Anna Depetris | 48bp |
| Ribosome binding site | BBa_K2927001 | RBS | Basic | KUO, PEI-CHING | 23bp |
| New Parts | |||||
| pelB signal sequence | BBa_K4223000 | Signalling | Basic | RongKai Tang | 66bp |
| DNA template of target RNA for LbuCas13a | BBa_K4223001 | DNA | Basic | RongKai Tang | 337bp |
| 6xHis tag | BBa_K4223002 | Tag | Basic | RongKai Tang | 18bp |
| Cas14a protein-coding gene | BBa_K4223004 | Coding | Basic | RongKai Tang | 1587bp |
| 10xHis tag | BBa_K4223005 | Tag | Basic | RongKai Tang | 30bp |
| Maltose Binding Protein (MBP) Tag protein | BBa_K4223006 | Tag | Basic | RongKai Tang | 1101bp |
| TEV Cleavage Site | BBa_K4223007 | Protein_Domain | Basic | RongKai Tang | 21bp |
| DNA template of mutated target RNA for LbuCas13a | BBa_K4223009 | DNA | Basic | RongKai Tang | 337bp |
| DNA template of sgRNA for Cas14a1 in vitro detection system | BBa_K4223011 | DNA | Basic | RongKai Tang | 187 bp |
| DNA template of crRNA for Cas13a1 in vitro detection system | BBa_K4223012 | DNA | Basic | RongKai Tang | 54bp |
| lac operator | BBa_K4223013 | Regulatory | Basic | RongKai Tang | 25bp |
| Thrombin cleavage site | BBa_K4223014 | Protein_Domain | Basic | RongKai Tang | 18bp |
| Twin-strep-tag | BBa_K4223015 | Tag | Basic | RongKai Tang | 84bp |
| SUMO protein tag | BBa_K4223016 | Tag | Basic | RongKai Tang | 291bp |
| Csm6 protein coding | BBa_K4223017 | Coding | Basic | RongKai Tang | 1392bp |
| Cas13a protein-coding gene | BBa_K4223019 | Coding | Basic | RongKai Tang | 3477bp |
| Transcription system of target RNA for LbCas13a | BBa_K4223003 | Translational_Unit | Composite | RongKai Tang | 515bp |
| Cas14a1 protein expression system | BBa_K4223008 | Composite | Composite | RongKai Tang | 2833bp |
| Transcription system of mutated target RNA for Cas13a1 | BBa_K4223010 | Composite | Composite | RongKai Tang | 553bp |
| A protein expression system of Csm6 | BBa_K4223018 | Composite | Composite | RongKai Tang | 1982bp |
| A protein expression system of Cas13a | BBa_K4223020 | Composite | Composite | RongKai Tang | 4067bp |