N-butanol, an organic compound(C4H10O), is a colorless transparent liquid. There is a larger demand for N-butanol than before. N-butanol can take advantage of its organic compounds to help transport hydrogen and reduce the waste of electricity, while also being a less polluting fuel. However, there are many disadvantages in the traditional N-butanol manufacturing process. Thus, our purpose is to produce N-butanol more efficiently by biosynthesis as well as be more friendly to the environment. Below is the method, Streptomyces acetobutyrate genes thl, hbd, crt and streptomyces dentatus ter need to be cloned into a plasmid expressing butanol synthetic pathway proteins. The plasmid carrying these genes with optimized codon will be introduced into the target strain through electrical transformation to test whether the transformants can successfully synthesize N-butanol. After that, the expression of the strain will be further optimized to successfully construct lactobacillus that could produce N-butanol using glucose as raw material.
