mApple is a luminescent protein, a variant of mOrange (E2050) discovered in 2008, that is substantially brighter than many other members of the RFP family. It is new to the iGEM registry and is the main reporter in our project.
A. caldus csoR is our best basic part because it was well documented with information regarding its characterization. It is a repressor sequence that functions in the copper buffer system of A. caldus. It was described and analyzed very recently, and we plan to explore its characteristics more.
PIII was identified as a possible promoter to pair with A. caldus csoR as a copper-sensitive operon. The resulting PIII-csoR system indeed induces strong transcription. However, it was reported to be non-copper-sensitive, a fact verified by our characterization of PIII. We therefore conducted mutation on the PIII-RBS system and constructed the K4494003-K4494098 mutant collection.
This is our Best Collection. Please refer to our Part Collection page for more information.
In order to improve an existing part to act as our benchmark, we characterized pCusC (K1980004). After the characterization procedure, we decided to further improve it. Our mutation generated 4 variants, three of which (K4494099-K4494101) do not function properly. However, K4494102 was a strong constant promoter, which could be seen as a successful improvement of K1980004.
This is the original sequence for our mutant collection K4494003-K4494098. It consists of PIII (K4494002) and an RBS (B0034) combined using Golden Gate Assembly. Since it has important significance in our project, we made it our Best Composite Part.
We requested this sequence from the authors of a paper on biosafety, but later abandoned the plan of using it due to changes in our project. However, we still thought that it might be useful to future iGEM teams who want to construct a biosafety circuit, so we kept the synthesized part to send to any future teams in need. This part is a member of the NWU (Northwest University) Part Library.
This is the transcriptional unit for A. caldus csoR that we used in our project. It works when combined with a corresponding promoter unit.
| Name | Type | Description | Designer | Length |
| BBa_K4494000 | Reporter | mApple | Shiran Yuan | 708 bp |
| BBa_K4494001 | Coding (Repressor) | A. caldus csoR | Shiran Yuan | 267 bp |
| BBa_K4494002 | Regulatory (Promoter) | PIII | Shiran Yuan | 58 bp |
| BBa_K4494003 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 1) | Shiran Yuan | 64 bp |
| BBa_K4494004 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 2) | Shiran Yuan | 87 bp |
| BBa_K4494005 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 3) | Shiran Yuan | 47 bp |
| BBa_K4494006 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 4) | Shiran Yuan | 85 bp |
| BBa_K4494007 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 5) | Shiran Yuan | 124 bp |
| BBa_K4494008 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 6) | Shiran Yuan | 84 bp |
| BBa_K4494009 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 7) | Shiran Yuan | 76 bp |
| BBa_K4494010 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 8) | Shiran Yuan | 82 bp |
| BBa_K4494011 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 9) | Shiran Yuan | 84 bp |
| BBa_K4494012 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 10) | Shiran Yuan | 79 bp |
| BBa_K4494013 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 11) | Shiran Yuan | 83 bp |
| BBa_K4494014 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 12) | Shiran Yuan | 75 bp |
| BBa_K4494015 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 13) | Shiran Yuan | 49 bp |
| BBa_K4494016 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 14) | Shiran Yuan | 56 bp |
| BBa_K4494017 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 15) | Shiran Yuan | 52 bp |
| BBa_K4494018 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 16) | Shiran Yuan | 82 bp |
| BBa_K4494019 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 17) | Shiran Yuan | 87 bp |
| BBa_K4494020 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 18) | Shiran Yuan | 52 bp |
| BBa_K4494021 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 19) | Shiran Yuan | 79 bp |
| BBa_K4494022 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 20) | Shiran Yuan | 85 bp |
| BBa_K4494023 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 21) | Shiran Yuan | 110 bp |
| BBa_K4494024 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 22) | Shiran Yuan | 86 bp |
| BBa_K4494025 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 23) | Shiran Yuan | 94 bp |
| BBa_K4494026 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 24) | Shiran Yuan | 52 bp |
| BBa_K4494027 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 25) | Shiran Yuan | 50 bp |
| BBa_K4494028 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 26) | Shiran Yuan | 124 bp |
| BBa_K4494029 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 27) | Shiran Yuan | 79 bp |
| BBa_K4494030 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 28) | Shiran Yuan | 49 bp |
| BBa_K4494031 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 29) | Shiran Yuan | 115 bp |
| BBa_K4494032 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 30) | Shiran Yuan | 52 bp |
| BBa_K4494033 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 31) | Shiran Yuan | 95 bp |
| BBa_K4494034 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 32) | Shiran Yuan | 72 bp |
| BBa_K4494035 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 33) | Shiran Yuan | 88 bp |
| BBa_K4494036 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 34) | Shiran Yuan | 51 bp |
| BBa_K4494037 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 35) | Shiran Yuan | 109 bp |
| BBa_K4494038 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 36) | Shiran Yuan | 81 bp |
| BBa_K4494039 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 37) | Shiran Yuan | 48 bp |
| BBa_K4494040 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 38) | Shiran Yuan | 47 bp |
| BBa_K4494041 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 39) | Shiran Yuan | 84 bp |
| BBa_K4494042 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 40) | Shiran Yuan | 120 bp |
| BBa_K4494043 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 41) | Shiran Yuan | 49 bp |
| BBa_K4494044 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 42) | Shiran Yuan | 72 bp |
| BBa_K4494045 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 43) | Shiran Yuan | 63 bp |
| BBa_K4494046 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 44) | Shiran Yuan | 49 bp |
| BBa_K4494047 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 45) | Shiran Yuan | 76 bp |
| BBa_K4494048 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 46) | Shiran Yuan | 90 bp |
| BBa_K4494049 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 47) | Shiran Yuan | 47 bp |
| BBa_K4494050 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 48) | Shiran Yuan | 47 bp |
| BBa_K4494051 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 49) | Shiran Yuan | 61 bp |
| BBa_K4494052 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 50) | Shiran Yuan | 64 bp |
| BBa_K4494053 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 51) | Shiran Yuan | 80 bp |
| BBa_K4494054 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 52) | Shiran Yuan | 82 bp |
| BBa_K4494055 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 53) | Shiran Yuan | 85 bp |
| BBa_K4494056 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 54) | Shiran Yuan | 52 bp |
| BBa_K4494057 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 55) | Shiran Yuan | 79 bp |
| BBa_K4494058 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 56) | Shiran Yuan | 83 bp |
| BBa_K4494059 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 57) | Shiran Yuan | 101 bp |
| BBa_K4494060 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 58) | Shiran Yuan | 52 bp |
| BBa_K4494061 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 59) | Shiran Yuan | 84 bp |
| BBa_K4494062 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 60) | Shiran Yuan | 82 bp |
| BBa_K4494063 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 61) | Shiran Yuan | 61 bp |
| BBa_K4494064 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 62) | Shiran Yuan | 47 bp |
| BBa_K4494065 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 63) | Shiran Yuan | 56 bp |
| BBa_K4494066 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 64) | Shiran Yuan | 73 bp |
| BBa_K4494067 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 65) | Shiran Yuan | 113 bp |
| BBa_K4494068 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 66) | Shiran Yuan | 53 bp |
| BBa_K4494069 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 67) | Shiran Yuan | 71 bp |
| BBa_K4494070 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 68) | Shiran Yuan | 106 bp |
| BBa_K4494071 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 69) | Shiran Yuan | 76 bp |
| BBa_K4494072 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 70) | Shiran Yuan | 82 bp |
| BBa_K4494073 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 71) | Shiran Yuan | 46 bp |
| BBa_K4494074 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 72) | Shiran Yuan | 109 bp |
| BBa_K4494075 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 73) | Shiran Yuan | 56 bp |
| BBa_K4494076 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 74) | Shiran Yuan | 85 bp |
| BBa_K4494077 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 75) | Shiran Yuan | 49 bp |
| BBa_K4494078 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 76) | Shiran Yuan | 59 bp |
| BBa_K4494079 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 77) | Shiran Yuan | 47 bp |
| BBa_K4494080 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 78) | Shiran Yuan | 61 bp |
| BBa_K4494081 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 79) | Shiran Yuan | 76 bp |
| BBa_K4494082 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 80) | Shiran Yuan | 65 bp |
| BBa_K4494083 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 81) | Shiran Yuan | 49 bp |
| BBa_K4494084 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 82) | Shiran Yuan | 86 bp |
| BBa_K4494085 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 83) | Shiran Yuan | 79 bp |
| BBa_K4494086 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 84) | Shiran Yuan | 64 bp |
| BBa_K4494087 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 85) | Shiran Yuan | 49 bp |
| BBa_K4494088 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 86) | Shiran Yuan | 82 bp |
| BBa_K4494089 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 87) | Shiran Yuan | 62 bp |
| BBa_K4494090 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 88) | Shiran Yuan | 56 bp |
| BBa_K4494091 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 89) | Shiran Yuan | 70 bp |
| BBa_K4494092 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 90) | Shiran Yuan | 77 bp |
| BBa_K4494093 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 91) | Shiran Yuan | 54 bp |
| BBa_K4494094 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 92) | Shiran Yuan | 56 bp |
| BBa_K4494095 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 93) | Shiran Yuan | 83 bp |
| BBa_K4494096 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 94) | Shiran Yuan | 79 bp |
| BBa_K4494097 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 95) | Shiran Yuan | 76 bp |
| BBa_K4494098 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR (2022 DKU_China Variant 96) | Shiran Yuan | 119 bp |
| BBa_K4494099 | Regulatory (Promoter) | pCusC (2022 DKU_China Variant 1) | Shiran Yuan | 47 bp |
| BBa_K4494100 | Regulatory (Promoter) | pCusC (2022 DKU_China Variant 2) | Shiran Yuan | 45 bp |
| BBa_K4494101 | Regulatory (Promoter) | pCusC (2022 DKU_China Variant 3) | Shiran Yuan | 26 bp |
| BBa_K4494102 | Regulatory (Promoter) | pCusC (2022 DKU_China Variant 4) | Shiran Yuan | 72 bp |
| BBa_K4494196 | Composite (Promoter+RBS) | Promoter-RBS Combination for A. caldus csoR | Shiran Yuan | 70 bp |
| BBa_K4494197 | Coding (Toxin) | Doc Toxin for Suicidal Modules | Shiran Yuan | 381 bp |
| BBa_K4494198 | Composite (Transcription Unit) | A. caldus csoR Transcriptional Unit | Shiran Yuan | 356 bp |