Our Human Practices work is very complete and integrated. In the brainstorming stage, we carefully studied the application scope and cases of synthetic biology and looked for projects around us. Through the residents‘ visit, we determined the project direction of heavy metal pollution and conducted a lot of literature research on it. Since the effectiveness of the literature is not ideal and comprehensive, we decided to interview stakeholders. We contacted 2 paper authors and interviewed 4 industry research experts, 6 enterprise experts, and 3 synthetic biology experts. The feedback from these experts helps us to constantly adjust our project plan and implementation method. This work makes us realize the correct process of academic research and engineering circle, and also provides a reference for others to carry out academic research and projects.
On our Model page, we presented two models. The first one is a theoretical calculation of the relationship between promoter activity and copper concentration in our project, in which we took toxicity-induced cell death into account. Due to a lack of data, we did not further investigate our experimental data with this model. However, it still led to many insights regarding our system, and we constructed an interactive demonstration of this model on our wiki page so that other teams can also use it. The second model encompasses experimental performance and genetic analysis to predict ideal variants for the future progression of our project to be based on. It also presented us with many meaningful insights regarding our parts, which can also be seen in our part documentations.
Our part collection was built via random mutation and characterized by high-throughput experimentation. The parts are well-documented as a rich range of data are provided to the readers, and the collection is also easy to use since our documentation of experimental protocols is complete. The collection also contains many parts for selection, and it also contains parts that do not function well for future teams to improve. This part collection contains variants of the csoR-PIII operon system.